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Biomedical subjects

H Haas

Publications and source records attributed to H Haas.

At least 19 recordsLinked to original sources
PubMed1992

Purification, characterization and partial amino acid sequences of a xylanase produced by Penicillium chrysogenum.

An extracellular xylanase (1,4-beta-D-xylan xylanohydrolase, EC 3.2.1.8, endo 1,4-beta-xylanase) was found to be the major protein in the culture filtrate of Penicillium chrysogenum when grown on 1% xylan. In contrast to other microorganism no xylanase multiplicity was found in P. chrysogenum under the conditions used. This enzyme was purified to homogeneity by high performance anion-exchange and size-exclusion chromatography. It had an M(r) of 35,000 as estimated by SDS-PAGE and was shown to be active as a monomer. No glycosylation of the protein could be detected neither by a sensitive glycostain nor by enzymatic deglycosylation studies. The enzyme hydrolyzed oat spelt and birchwood xylan randomly, yielding xylose and xylobiose as major end products. It had no cellulase, CMCase, beta-xylosidase or arabinogalactanase activity but acted on p-nitrophenylcellobioside. The pH and temperature optima for its activity were pH 6.0 and 40 degrees C, respectively. Eight peptides obtained after endoproteinase LysC digestion of xylanase have been sequenced, six of them showed considerable amino acid similarity to glucanases and high M(r)/acidic xylanases from different bacteria, yeasts and fungi.

Amino Acid Sequence↗
PubMed1992

Calcium-dependent and calcium-independent signals in the conglutinin-binding assay (KgBa) for immune complexes. Influence of anti-collagen-antibodies.

A solid phase ELISA conglutinin-binding assay (KgBa) was evaluated for the detection of circulating immune complexes. ELISA wells were coated with purified bovine conglutinin and incubated with test sera. Bound IgG was detected with enzyme labelled anti-immunoglobulin. Heat aggregated IgG which had been "solubilized" (i.e., complement treated by incubation with serum) was employed as a reference. The binding of the complement-reacted IgG to solid phase conglutinin was found to be calcium-dependent and inhibitable with N-acetyl-D-glucosamine (GlcNAc). Prolonged incubation (4 days) of aggregated IgG with serum at 37 degrees C abolished the binding to conglutinin, a finding consistent with the complete degradation of deposited C3b to C3c and C3d. The solubilized IgG that bound to solid phase conglutinin was found by gel chromatography to be of high molecular weight (greater than 600 kDa). Binding of IgG to solid phase bovine conglutinin was also observed to a variable degree in normal and pathological sera. However, in this situation the IgG binding was largely calcium-independent, was not inhibited by GlcNAc and did not decrease after prolonged incubation of the serum at 37 degrees C. The reactive IgG eluted on gel chromatography at the position of monomeric IgG suggesting binding via the antigen binding sites. Binding of this IgG was inhibited by both collagen type II and purified conglutinin. These observations suggest that the assay detects cross-reacting autoantibodies against collagen epitopes, or, alternatively, antibodies against the dietary antigen, bovine conglutinin.

Acetylgalactosamine↗
PubMed1992

Isolation and analysis of the Penicillium chrysogenum phoA gene encoding a secreted phosphate-repressible acid phosphatase.

We have isolated the genomic sequence encoding a secreted phosphate-repressible acid phosphatase (PHOA) from Penicillium chrysogenum using synthetic oligodeoxyribonucleotide probes. Nucleotide sequence data revealed that this gene consists of two exons of 192 and 1047 bp separated by an intron of 52 bp in length. A sequence encoding a putative signal peptide, resembling known signal sequences of fungi, was identified at the 5'-end of the coding sequence. Northern blot analysis of total cellular RNA indicated that the phoA gene codes for a 1.6-kb transcript. The expression of this gene is regulated at the transcriptional level and is markedly affected by the inorganic phosphate concentration of the growth medium.

Acid Phosphatase↗
PubMed1992

Characterization of a human-human hybridoma antibody, C-OU1, directed against a colon tumor-associated antigen.

The human hybridoma cell line, B9165, was obtained after fusion of lymphocytes from lymph nodes draining the tumor region in a patient with adenocarcinoma of the colon with the human B-lymphoblastoid cell line WI-L2-729-HF2 (729-HF2). B9165 secretes the human monoclonal antibody, C-OU1 (IgM, kappa). Immunocytochemical and immunohistochemical analysis showed that the antibody bound to a differentiation antigen. Electron microscopy of colonic adenocarcinoma cells, intact tumor and colonic epithelium by the immunogold technique demonstrated that the C-OU1 antibody reacted with a molecule associated with areas of disruption of the intermediate filaments in the cytoplasm of the tumor cells. No reaction was seen with intermediate filaments in normal colonic epithelium. The molecular weight of the antigen was shown to be 43 Kda by SDS-PAGE and Western blotting of tumor extracts, and isoelectric focusing of sonicated extracts demonstrated reaction with molecular species of pI 5.4-6.2. These findings suggest that the C-OU1 antigen is a modified cytokeratin 18. The B9165 cell line has proved to be quite stable, and the antibody is of potential clinical value. Its usefulness for localizing tumors in patients is being investigated.

Antibodies, Monoclonal↗
PubMed1992

Augmentation of the antibacterial activity of magainin by positive-charge chain extension.

Novel analogs of the broad-spectrum antimicrobial peptide magainin-2 were obtained by extension of its chain through addition of segments of positively charged amino acids to either its N or its C terminus and by increasing its helicity. The activity of magainin-2 toward American Type Culture Collection strains of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus was most considerably enhanced by these modifications, whereas, in general, its low hemolytic capacity was not or was only slightly affected. The antibacterial potencies of magainin-2 and its derivatives were more evident following decreases of pH from 7.2 to 6 and 5.

Amino Acid Sequence↗
PubMed1992

[The severely injured patient in the emergency center--new diagnostic, therapeutic and organizational concepts].

Recent advances in clinical intensive care medicine and legal aspects of quality assurance require new concepts and methods in preclinical emergency medicine. Different new methods of emergency medicine are presented and evaluated as to their economy, practicability, and medical efficacy, points which have to be considered in order to make general recommendations.

Cost-Benefit Analysis↗
PubMed1991

Prevalence and subclass distribution of IgG-anti-IgE autoantibodies in atopy and parasitosis.

Using immunoblotting, we investigated sera of 60 patients with atopic dermatitis, 12 patients with helminth infections and 36 nonallergic controls, for anti-IgE autoantibodies. We found IgG anti-IgE autoantibodies in 62% of the atopics, 42% of the patients with parasitosis and 11% of the controls. IgG anti-IgE occurred most often (94%) in patients with atopic dermatitis plus additional atopic disorder, such as allergic asthma and rhinoconjunctivitis. In parallel, we found a significantly higher occurrence of IgG anti-IgE in the patients with high IgE levels compared to patients with low IgE levels (p less than 0.0005). The predominant subclasses of anti-IgE autoantibodies were IgG1 and IgG3 in atopy and parasitosis. In the controls, we found IgG4 and IgG3 anti-IgE, but no IgG1 autoantibodies. The frequency of IgG2 anti-IgE was very low; it occurred in 2 patients only. Prevalence and IgG subclass distribution of anti-IgE autoantibodies was found to be different for patients with atopic dermatitis, parasitic infection and for controls.

Antibodies, Anti-Idiotypic↗
PubMed1990

Differential regulation of HLA class I genes by interferon.

Allele-specific differences in the regulation of HLA class I genes by type I interferon (IFN) were observed after transfection of eight HLA-B, -A, or -C genes into mouse L cells. HLA-B7 and -Bw64 gene expression was significantly more inducible by type I IFN than the genes coding for HLA-B27, HLA-B51, HLA-B38, HLA-B39, HLA-Cw3, and HLA-A2 antigens. Modification of the 5' end of HLA-B7 and HLA-B27 genes revealed the presence of enhancer sequences responding to IFN treatment in the 5' untranslated region of HLA-B7, but not of HLA-B27 and suggested further, independently acting enhancer elements downstream of the transcription initiation site. Comparison of 5' enhancer region sequences in correlation with type I IFN inducibility of the different HLA class I alleles indicated that the exchange of only two nucleotides in the interferon response sequence (IRS) or enhancer A region of HLA-B7 or -Bw64 could account for nonregulated promoters in all other HLA-A, -B, or -C alleles analyzed. Thus, type I IFN stimulation of HLA class I genes in mouse L cells appears to predominantly operate in most alleles by a mechanism targeted to enhancer sequences downstream of the gene's transcription initiation site.

Alleles↗
PubMed1979

[Acute lymphatic leukaemia in children: re-examination after remission of at least five years (author's transl)].

Full remission of more than five years was observed in nine of 24 children with acute lymphatic leukaemia. They had been treated by a scheme similar to the Pinkel VII one, and all had been discharged from treatment. The immune status, bone marrow, radiological, neurological and psychosomatic studies, as well as various laboratory tests indicated that in seven of the children the findings, are essentially normal. But two children have lasting severe organ damage, portal fibrosis in one and bilateral intracerebral calcification in the other, both presumably due to the methotrexate treatment.

Adolescent↗
PubMed1979

Implantation of cigarette smoke condensate in the lungs of Syrian golden hamsters.

Different doses of benzo(a)pyrene, cigarette smoke condensate (CSC) and its nitromethane fraction (NMF), in wax pellets, were directly implanted into the lungs of Syrian golden hamsters. Pleomorphic sarcomas were induced in the B(a)P treated animals. Several epithelial proliferative lesions and a few bronchiogenic adenomas were induced in CSC and NMF treated hamsters. A species-specific effect has been observed in this investigation.

Adenoma↗
PubMed1979

Antibody against hepatitis A in seven European countries. I. Comparison of prevalence data in different age groups.

Using a solid phase radioimmunoassay, antibody to hepatitis A virus (anti-HAV) was determined in 3890 sera from populations in seven European countries. Prevalence of anti-HAV was lowest in Scandinavian countries and highest in Greece and France. Antibodies were found in 77 (13%) of 602 blood donors in Sweden, in 29 (17%) of 175 blood donors and women taking birth control pills in Norway, in 273 (39%) of 700 blood donors in Switzerland, in 262 (52%) of 505 blood donors in Holland, in 365 (55%) of 661 accident patients in West Germany, in 452 (75%) of 600 blood donors in France and in 530 (82%) of 647 persons in Greece. Prevalence of anti-HAV increased with age in all populations tested, indicating nearly total exposure to HAV in persons over 19 years of age in Greece and in persons over 39 years of age in West Germany, Holland and France. Antibody was found more frequently in rural than in urban populations in Greece and Switzerland. Calculation of the age-specific incidence of HAV infections suggests a remarkable decline in the exposure rate in the last few decades.

Adolescent↗
PubMed1979

[Plasma cell granuloma of the lung (author's transl)].

Case-report of a plasma cell granuloma of the lung found in a girl aged 8 years 6 months. The lesion of the lung was first interpreted as a metastasis of a suspected primary tumor in the right calf. It was only later that plasmacell granuloma of the lung was diagnosed. Plasma cell granuloma of the lung often gives rise to confusion, a fact illustrated by the various names applied to it, e.g. pseudosarcoma, postinflammatory pseudotumor of the lung, histiocytoma, xanthoma, fibroxanthoma, xanthogranuloma. On histological examination, this tumor consists of proliferations mainly of plasmacells and reticulo-endothelial cells, with some polymorphism and some giant cells. The tumor appears as a single, sharply circumscribed mass, is usually asymptomatic and therefore found mostly on routine-chest-films. Rarely is it found as an obstructive mass in the bronchi or in the trachea. The histopathogenesis of the tumor is not known, the lesion benign. Treatment is resection.

Child↗
PubMed1979

[Asplenia and DIC (author's transl)].

3 cases of severe septic shock are described; a 5 month old girl with congenital hyposplenia, a 2 3/12 year old boy splenectomized because of microspherozytosis and a 11 6/12 year old boy splenectomized because of Hodgkin's disease. In 2 cases pneumococci were found in the blood cultures. In all 3 cases the coagulation analysis showed a consumption coagulopathy. Intravenous streptokinase treatment was applied in addition to general treatment for shock and antibiotic therapy. 2 patients survived and made a complete recovery, whereas the 2 year old boy died. The histological findings showed a severe DIC. In the Department of Surgery, Innsbruck, 44 children have been splenectomized during the last 6 years, 38 of whom we were able to follow up on for an average of 3.3 years. After an average of 1.2 years following splenectomy, 4 patients (including the 3 cases mentioned above) contracted acute septicaemia; a further patient also incurring a probable sepsis with DIC. 3 of these 5 children died, representing a morbidity rate of 13% and a mortality rate of 8%. The mortality rate is thus as high as that caused by the primary disease, indicating the urgency of prophylaxis for infections of this kind. 3 prophylactic forms of treatment are suggested: protection with penicillin, active immunization with polyvalent pneumococcal antigen and spleen preservation whenever possible.

Bacterial Infections↗
PubMed1978

[Basilar or vertebral artery aneurysm as a cause of presumed cervical spine injury (author's transl)].

The characteristic clinical features and morphological findings of five cases of (clinically not diagnosed) fusiform aneurysm of the basilar or vertebral arteries were recurring attacks of positional occipital headache, pain and stiffness in the neck, cranial nerve disturbances, expecially oculomotor palsies and anisokoria, nystagmus, attacks of nausea, vomiting and sweating, tachycardia, pyramidal tract symptoms, and pareses. Severe hypertension had been present in four instances. The aneurysm, which is usually thrombosed, pressed against the pons and medulla oblongata as a space-occupying mass. In addition to hypertension and atheromatosis, congenital defect in the arterial wall are probably significant causative factor. To mistake an aneurysm for a cervical syndrome may be fatal to the patient.

Adult↗
PubMed1978

Detection of HBeAg and anti-HBe in acute hepatitis B by a sensitive radioimmunoassay.

A solid-phase radioimmunoassay using anti-HBe-coated polysterence beads and iodine-125-labeled anti-HBe of human origin was developed for the detection of HBeAg. Anti-HBe could be determined by a blocking test. Both assays were about 500-fold more sensitive than immunodiffusion. Few nonspecific positive results for HBeAg could be recognized in the anti-HBe test by increase in cpm over that of the negative control. HBeAg was not found in acute hepatitis A and non A-non B hepatitis or in a control group of accident patients. On admission to the hospital 12 of 48 (25%) acute hepatitis B patients from Greece and 17 of 20 (85%) acute hepatitis B patients from Germany were HBeAg-positive. All 39 initially HBeAg negative sera were already anti-HBe positive. Tests of the acute stage and follow-up sera of the 20 German patients indicated that HBeAg is regularly present in the incubation period and early acute phase of hepatitis B. After onset of disease the antigen is cleared from the serum very rapidly in uncomplicated cases and is usually followed by the appearance of anti-HBe. Like anti-HBc, anti-HBe can serve as a tool for the diagnosis of hepatitis B after the disappearance of HBsAg.

Acute Disease↗