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H Haapasalo

Publications and source records attributed to H Haapasalo.

67 records · Page 4Linked to original sources
PubMed1992

DNA ploidy, S-phase fraction, and G2 fraction as prognostic determinants in human pancreatic cancer.

The DNA ploidy, S-phase fraction (SPF), and G2 fraction of pancreatic cancer tissue was measured by flow cytometry in 95 patients. Forty-nine per cent (n = 47) had a diploid DNA index, and 51% (n = 48) of tumours were aneuploid. Aneuploid tumours and high-grade tumours had significantly higher S-phase and G2-fraction values than diploid tumours or low-grade tumours. Diploid and tetraploid tumours had a more favourable prognosis than non-tetraploid aneuploid tumours (p = 0.0020) during the mean follow-up of 6 years. The type of therapy (p = 0.07), histologic grade (p = 0.06), SPF (p = 0.1), and G2 fraction (p = 0.02) had predictive value in survival analysis as well. In multivariate survival analysis, including flow-cytometric, histologic, and clinical variables, diploidy and tetraploidy had independent predictive value. The results suggest that flow cytometry might be used in grading of pancreatic cancer. Such a grading would have practical value if new modes of therapy are being developed. Forty-one per cent of multiple samples had a heterogeneous DNA index when multiple samples were used. Consequently, flow cytometric analysis of pancreatic cancer using multiple samples is recommended.

Adenocarcinoma↗
PubMed1991

Prognostic factors in human pancreatic cancer, with special reference to quantitative histology.

A retrospective clinicopathologic study was done on 111 patients with a pancreatic ductal adenocarcinoma. The mean follow-up period was 6 years. By means of interactive morphometry six nuclear morphometric features were measured in biopsy specimens from the primary tumours. Volume-corrected mitotic index (M/V index) was estimated in the same sections. Histologic grading was done in accordance with the WHO. The M/V index (p = 0.002), the nuclear area of the 10 largest nuclei (NA10) (p = 0.025), the histologic grade (p = 0.0956), the nuclear area (NA) (p = 0.038), the standard deviation of the nuclear perimeter (SDPE) (p = 0.033), and the standard deviation of the nuclear area (SDNA) (p = 0.0430) predicted survival in univariate analysis. The type of surgery performed was a significant prognosticator too (p = 0.0131). A multifactor regression analysis of survival including clinical and histologic factors identified the M/V index as the most important prognosticator (p = 0.009), followed by the type of surgery performed (p = 0.022). Other histologic factors had no independent prognostic value. Our results suggest the use of morphometric features instead of the conventional histologic grading in predicting survival of pancreatic ductal adenocarcinoma.

Aged↗
PubMed1991

Tumour ploidy, morphometry, histological grading and clinical features in ovarian carcinoma: mutual relations.

The relationship between tumour ploidy and qualitative and quantitative histopathology was assessed in a series of 95 ovarian carcinomas. 67% of the tumours were non-diploid (DNA aneuploid). 56% of the early stage (I-II) tumours were non-diploid and 81% of the tumours in advanced (III-IV) stages were aneuploid. Histological grading failed to show a clear relationship between increasing malignancy grade and ploidy. There was a close association between DNA ploidy and nuclear perimeter, area and shortest and longest nuclear diameter: the nuclei of non-diploid tumours were generally larger. Also the number of mitotic figures per square millimeter of epithelium in the microscope image (volume-corrected mitotic index, M/V-index) differed significantly between near-diploid and non-diploid tumours. Discriminant analysis showed that 74% of the learning-set tumours (67% of the test set tumours) could be correctly classified in low-ploidy and high-ploidy categories with morphometric features (nuclear perimeter, M/V-index and volume percentage of epithelium). Characteristic features of non-diploid ovarian tumours--rapid proliferation and large nuclear size--could be assessed with morphometric methods which allowed a relatively large aneuploid tumour group to be distinguished.

Chromosomes↗
PubMed1990

Consistency of quantitative methods in ovarian tumor histopathology.

Two mitotic activity indices, volume fraction of neoplastic epithelium, nuclear area, nuclear perimeter, and shortest nuclear axis were estimated in 46 ovarian tumors by three observers in two independent laboratories. The mitotic activity index, the volume corrected mitotic index (M/V index) and subjective volume fraction estimates showed a very good correlation from the same fields by two observers in the same laboratory (r = .999, .995, .950). Repeat estimates from different fields by the same observer showed coefficient values of .949, .939, and .813, and estimates by two different observers within one laboratory values of .949, .936, and .789, respectively. The correlation between two independent observers in different laboratories was also good (r = .894, .834, .834, respectively). Grading based on morphometric measurements was uniformly performed in 90-100% of cases in an interfield interobserver intralaboratory situation, and in 79-97% (M/V index) or 76-93% (mitotic activity index) in an interlaboratory situation. Because the cases near the grade limits were more often differently graded than other cases, the methods allowed the pathologist to locate the probably incorrectly graded cases. Morphometry of ovarian tumors was shown to be easy, which makes morphometric malignancy grading systems potentially able to support diagnostic and therapeutic decisions in practice.

Cell Nucleus↗
PubMed1990

Prognostic value of ovarian carcinoma grading methods--a method comparison study.

The prognostic value of subjective histological and morphometric grading was studied in 75 primary ovarian carcinomas. Histological grading methods recommended by Czernobilsky and by Russell and the morphometric method of Baak and co-workers were compared in a two-observer system. The 5-year survival could be correctly predicted in about two-thirds of the patients with all three methods. When mitotic counting (volume corrected mitotic index, M/V-index) was compared with the above grading methods by using a receiver operating characteristic curve) the M/V-index was generally superior in its prognostic power regardless of the sensitivity/specificity level chosen. The morphometric grading method and the grading method based on the M/V index were also shown to be readily reproducible.

Carcinoma↗
PubMed1989

Volume corrected mitotic index (M/V-INDEX). The standard of mitotic activity in neoplasms.

Estimation of mitotic activity by counting the number of mitotic figures per high power fields is subject to many sources of error. The microscope fields vary in size in different microscopes, which makes comparisons arbitrary. Also the areas covered by neoplastic and non-neoplastic tissue in the microscope image vary in different tumors. We found that each microscope could easily be characterized in terms of the size of the microscope field at 40x objective magnification (usually at ocular magnification of 10x). We also found that the number of mitotic figures can be related to the area covered by neoplastic tissue in the microscope field. The resulting index, the volume corrected mitotic index (M/V-index) will express the mitotic activity as the number of mitotic figures per square millimeter of neoplastic tissue in the microscope fields. The M/V-index will not be subject to the variation in volume fractions of neoplastic tissue between different neoplasms. With the M/V-index the mitotic activity as measured in different microscopes can be compared reliably. The value of the method is demonstrated in a series of ovarian tumors.

Adenocarcinoma↗
PubMed1989

Prognosis of ovarian carcinomas: prediction by histoquantitative methods.

Prediction of prognosis of ovarian carcinomas by morphometric, histopathological and clinical indices was estimated in 105 tumours. Morphometric parameters included mitotic activity index, volume-corrected mitotic index (M/V index), volume fraction of neoplastic epithelium, nuclear area, nuclear perimeter, shortest and longest nuclear axis and form factor of nucleus. Cox's multivariate regression model showed that the clinical stage was the best predictor of prognosis followed by the M/V index, which expresses the mitotic activity as the number of mitotic figures per square millimeter of neoplastic epithelium in the microscope field. In all tumour subgroups studied the M/V index was the best prognostic factor and for stage I tumours it was the only parameter selected by the Cox's model as a significant and independent prognostic predictor. We conclude that the M/V index can be used as a significant prognostic factor in ovarian carcinomas.

Carcinoma↗
PubMedDate not supplied

The site-specific effects of long-term unilateral activity on bone mineral density and content.

This study assessed the site-specific effects of long-term tennis playing on the bone mineral density (BMD) and content (BMC) of upper extremities in male Finnish top-level players using a dual energy x-ray absorptiometric (DXA) scanner. In players (n = 20), the BMDs and BMCs were significantly higher in each bone of the playing right extremity (p < 0.05-0.001), the side-to-side difference being largest in the humeral shaft (BMD + 25.4%, BMC + 28.7%) and proximal humerus (BMD + 14.4%, BMC + 20.5%), and smallest in the ulnar shaft (BMD + 3.1%, BMC + 7.5%) and distal ulna (BMD + 6.3%, BMC + 7.8%). In sex-, age-, weight-, and height-matched controls (n = 20), the right-to-left differences were small ranging from 0.0% to + 6.4% (average +3%). The number of training sessions per week was the only variable in muscle strength and training history assessment which showed, in several anatomic sites, a significant correlation with the relative bone mineral variables (r = 0.460-0.627, p < 0.05-0.001). In conclusion, long-term unilateral tennis activity had a clearly positive effect on the BMD and BMC of the bones of the playing extremity. The effect was very site-specific, being greatest in the humerus and smallest in the ulna. The effect was always greater in BMC than BMD indicating that the excess mineral was used not only for condensation of the bone tissue, but also for enlargement of bone dimensions.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMedDate not supplied

Delayed MR imaging changes in acute disseminated encephalomyelitis.

BACKGROUND AND PURPOSE: White matter lesions on MR images obtained from patients with acute disseminated encephalomyelitis (ADEM) have been reported to appear shortly after symptom onset, and their resolution has been claimed to parallel recovery. To elucidate the temporal evolution of these lesions and to associate the changes on MR images to the patients' clinical condition, we performed serial MR imaging on patients with ADEM. METHODS: Several consecutive T2-weighted and fluid-attenuated inversion recovery scans were obtained from four previously healthy adult patients with ADEM within the first days after the onset of symptoms and again during the recovery period. MR imaging was done first on a weekly to biweekly basis and later at 1- to 2-month intervals for up to 8 months. RESULTS: MR scans of three of these patients did not show any specific abnormalities until several weeks after the onset of the disease. As the lesions later appeared, their number increased during the recovery period. CONCLUSION: MR imaging performed during the first days after the onset of the disease may not reveal any pathologic findings. The appearance of the ADEM-associated MR imaging changes may be associated with recovery rather than decline. It remains to be studied whether the new MR imaging techniques reveal the lesions associated with ADEM faster than the conventional T2-weighted imaging.

Adult↗