Biomedical subjects

H Håkanson

Publications and source records attributed to H Håkanson.

5 recordsLinked to original sources

PubMed1992

Process monitoring by flow-injection immunoassay. Evaluation of a sequential competitive binding assay.

A new variation on the theme of flow-injection binding assays is presented, namely the sequential competitive binding assay, in which the sample containing the native antigen is first introduced into the flow system followed by a pulse of labelled antigen. The flow-injection binding assay was used in monitoring the effluent from column chromatographic separations of proteins. By applying a computer-based evaluation system, concentrations are automatically read and compensation is made for denaturation in the affinity sorbent.

Binding, Competitive↗

PubMed1992

Immunochemically based assays for process control.

Immunoanalysis for process control is reviewed. The development of non-equilibrium immunoassays from a manually operated flow injection system to a completely computerized system for sample analysis as well as calibration and data evaluation is discussed. Several other approaches in using immunochemistry in biosensors for process control are discussed. The fact that reuse of antibodies raises specific demands on their properties is discussed. Even if the area is young, much progress has been achieved and one can foresee an interesting future development of the area.

Biomedical Engineering↗

PubMed1991

Continuous monitoring of urea in blood during dialysis.

Urease was immobilized to porous glass and used in combination with a conductivity meter for determining urea in standard solutions as well as in blood from a patient undergoing dialysis. The sampling unit involves a possibility for heparinization at the sampling point and a dialysis step prior to exposure to the enzyme column. The unit operates in a linear mode in the concentration range 5-50 mM. Monitoring of dialysis process gave good correlation with off-line analyses.

Biosensing Techniques↗

PubMed1979

Comparison of the hearing threshold measured by pure-tone audiometry and by Békésy sweep audiometry.

The hearing thresholds of 115 subjects, workers in a shipyard, were determined both by Békésy sweep audiometry and by conventional individual pure-tone audiometry at fixed audiometric frequencies. The Békésy method gave the lowest values for the hearing thresholds. It has been possible to find a useful linear relation between pure-tone and Békésy hearing thresholds. With the help of a retest experiment it has been established that the standard deviations of hearing thresholds, obtained under similar conditions in a pure-tone investigation, are about twice as large as those obtained in a Békésy investigation.

Adult↗

PubMed1979

Comparison of the hearing threshold measured by manual pure-tone and by self-recording (Békésy) audiometry.

The hearing threshold of 115 subjects aged 25--63 years and working on a shipyard were determined both by Békésy sweep audiometry and by conventional manual octave pure-tone audiometry at fixed audiometric frequencies. The attenuation rate was 2.5 dB s-1 with pulsed-tone presentation and the sweep time from 0.25 to 10 kHz was 400 s for the Békésy audiometer. Manual pure-tone audiometry was performed in 5-dB steps. The Békésy method gave the lowest values for the hearing thresholds. It has been possible to find a useful linear relation between pure-tone and Békésy hearing thresholds. With the help of a retest experiment, it has been established, that the standard deviations of hearing thresholds obtained under similar conditions in a pure-tone investigation are about twice as large as those obtained in a Békésy investigation.

Adult↗