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H H Conaway

Publications and source records attributed to H H Conaway.

30 records · Page 2Linked to original sources
PubMed1976

Ionophore A23187-induced insulin secretion in the isolated, perfused dog pancreas.

Perfusion of ionophore A23187 (10 muM) in the isolated dog pancreas resulted in a monophasic release of insulin. Ionophore A23187 (10 muM) failed, however, to elicit insulin secretion when added to calcium deficient (0.1 mmoles/L) perfusate. Simultaneous reintroduction of calcium (1.27 mmoles/L) and discontinuance of ionophore A23187 following calcium deficient periods caused a monophasic secretion of insulin which was quantitatively very similar (41,400 +/- 13,800 muU) to that stimulated by ionophore during normocalcemic perfusion. With reference to the current literature, these results suggest that ionophore A23187 elicits insulin secretion in the perfused dog pancreas preparation by increasing the level of free intracellular calcium, a process which is dependent upon a normal extracellular ionic calcium concentration.

Animals↗
PubMed1975

Characterization of acetylcholine-induced insulin secretion in the isolated perfused dog pancreas.

In the presence of a nonstimulatory concentration of glucose, a 60-min perfusion with 50 muM acetylcholine was shown to elicit a monophasic release of insulin in the isolated dog pancreas preparation. A decline in secretory response, which may be due to desensitization of the beta-cell to acetylcholine, was noted during the latter part of the perfusion interval. The potent insulin secretory response elicited by acetylcholine during the 60-min period was abolished 0y 25 muM atropine. Inhibition of the insulinotropic action of acetylcholine was also noted with administration of the mitotic spindle inhibitor, colchicine. When compared to 20-min control perfusions, addition of 1 mM colchicine resulted in a 50% reduction in acetylcholine-induced insulin release. These results suggest that insulin secretion stimulated by acetylcholine can be considered to be due to a muscarinic action of this agent which is dependent, at least in part, upon the microtubular system of the beta-cell.

Acetylcholine↗
PubMed1972

Calcitonin.

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Animals↗
PubMedDate not supplied

Spontaneous diabetes mellitus in the New Zealand white rabbit: history, classification, and genetic analysis.

A colony of NZW rabbits was developed in which 18 of 126 members exhibited overt symptoms of diabetes mellitus. On the basis of total body weight measurements, obesity does not appear to play a primary role in the development or manifestation of the syndrome. The relatively high frequency of occurrence of spontaneous diabetes mellitus in this colony seems to suggest a unique genetic predisposition of these rabbits, yet analysis of glucose tolerance of colony animals indicates no clear genetic mode of transmittance of the trait. Rather, data suggest a possible interaction of as yet undefined genetic and/or environmental influences as being responsible for the disease state. Regressions of k-value on age indicate an early predetermination of glucose intolerance in the rabbits. In addition to a planned program of breeding, investigations of dietary intake and possible relationships of the diabetic condition to bacterial or viral infections appear to be initial areas indicative of further study.

Animals↗