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Biomedical subjects

H Grant

Publications and source records attributed to H Grant.

At least 37 records · Page 2Linked to original sources
PubMed1994

Expression of liver functions in immortalised rat hepatocyte cell lines.

The differentiated hepatic function of two rat liver cell lines, P9 and SV40RH1, immortalised by transfection with SV40 DNA has been investigated in terms of the glutathione synthesis, and the activities of gamma-glutamyltransferase, glutathione-S-transferase and UDP-glucuronosyltransferase. SV40RH1 is a highly differentiated cell line at early passage, but the expression of some aspects of its differentiated phenotype is unstable and some functions are lost by passage 12-13. P9 is a less-well differentiated cell line, with relatively stable expression of functions between passages 4 and 13. In terms of differentiated function both cell lines represent a marked improvement over primary cultures of rat hepatocytes which de-differentiate rapidly within 24-48 h in culture. This retention of liver function in proliferating cell lines offers the opportunity to use such cells in in vitro toxicological studies.

Animals↗
PubMed1989

Osteoma of the incus.

Osteomas of the temporal bone are rare. Osteomas of the middle ear have been reported but none involving an ossicle. We describe a case of a 28-year-old man who had a conductive hearing loss caused by an osteoma of the incus.

Adult↗
PubMed1983

The effect of opioid drugs on the release of dopamine and 5-hydroxytryptamine from rat striatum following activation of nicotinic-cholinergic receptors.

The effect of (Met5)enkephalin, (D-Ala2,D-Met5)enkephalin, (Leu5)enkephalin, (D-Ala2,D-Met5)enkephalin and morphine on the release of [3H]dopamine, endogenous dopamine and [3H]5-hydroxytryptamine produced by the nicotinic-cholinergic agonist, dimethylphenyl piperazinium iodide (DMPP), was examined in rat striatal slices. The DMPP-induced release of [3H]dopamine and endogenous dopamine was reduced by the presence of (Met5)enkephalin, (D-Ala2,D-Met5)enkephalin (1-10 microM) or morphine (10 microM) but not by (Leu5)enkephalin or (D-Ala2,D-Leu5)enkephalin. The DMPP-induced release of [3H]5-hydroxytryptamine was reduced by (Leu5)enkephalin, (D-Ala2,D-Leu5)enkephalin, (Met5)enkephalin, (D-Ala2,D-Leu5)enkephalin (1-10 microM), and morphine (10 microM). All three opioids failed to alter the release of [3H]dopamine induced by field stimulation or potassium depolarization (30 microM). The inhibitory effects of opioid peptides and morphine demonstrated in the present study appear to be due to an initial interaction with nicotinic-cholinergic receptors in the striatum.

Animals↗
PubMed1983

Release of dopamine and 5-hydroxytryptamine from rat striatal slices following activation of nicotinic cholinergic receptors.

1. The administration of the nicotinic cholinergic agonists dimethylphenylpiperazinium iodide (DMPP) or nicotine caused a concentration dependent release of [3H] dopamine, [3H]5-hydroxytryptamine as well as endogenous dopamine and 5-hydroxytryptamine from superfused slices of rat striatum. 2. Release of both labelled and non-labelled transmitter was antagonized by the nicotinic antagonist, hexamethonium but not the muscarinic antagonist, atropine. 3. The present study provides additional evidence that nicotinic-cholinergic receptors are present in the mammalian central nervous system. 4. Activation of these nicotinic-cholinergic receptors in the striatum results in the release of both dopamine and 5-hydroxytryptamine.

Animals↗
PubMed1980

Cerebral damage in paraquat poisoning.

This is the first report on cerebral changes in eight patients who died of paraquat poisoning. These included generalized oedema, haemorrhages (both subependymal and subarachnoid), glial reactions (microglial activity and astrocytic response) and meningeal inflammation. Oedema and haemorrhage were the most consistent and significant findings; they suggest that paraquat may damage the cerebral blood vessels. The distribution of haemorrhages was unusual and resembled that seen in thiamine deficiency.

Adolescent↗
PubMed1980

[The phagocytic activity (ingestion and bactericidal activity) of neutrophil leukocytes in chronic myelosis].

The paper deals with the results obtained by investigating the function of phagocytes (ingestion and bactericidity) in germs of a stem of staphylococcus aureus 209 and escherichia coli 0-III of mature neutrophilic granulocytes in 30 patients with chronic, not-lymphocytic leukaemia. The ingestion corresponded to the norm. Bactericidity towards the germs of staphylococcus aureus was statistically significant decreased, viz. it was retarded as well as diminished. In certain patients this deviation from the norm found a particularly strong expression, which became evident in clinical appearances. As it was to be expected, no interconnection could be detected between the activity of alkaline granulocytic phosphatase and the function of phagocytosis.

Alkaline Phosphatase↗
PubMed1977

Severe head injuries in three countries.

Methods for assessing early characteristics and late outcome after severe head injury have been devised and applied to 700 cases in three countries (Scotland, Netherlands, and USA). There was a close similarity between the initial features of patients in the three series; in spite of differences on organisation of care and in details of management , the mortality was exactly the same in each country. This data bank of cases (which is still being enlarged) can be used for predicting outcome in new cases, and for setting up trials of management.

Adolescent↗
PubMed1977

[Leukocyte lysozymes in various forms of leukemia].

The amount of lysozyme in the leukocytes of 47 patients with different forms of leukaemia and 6 healthy persons was investigated. The lysozyme determination was carried out in the lysate of isolated leukocytes obtained after freezing and thawing it seven times. The results expressed in microgram per 10(6) cells were compared with the simultaneously determined lysozyme concentration of serum and urine. A substantial reduction of the lysozyme amount as compared with the normal value (3.1 microgram/10(6) cells) was determined in the leukocytes of patients suffering from chronic lymphatic leukaemia, acute lymphatic leukaemia and the blastic crisis of chronic myeloid leukaemia. Different amounts of lysozyme ranging from extremely low ones to strongly elevated ones were found in leukocytes taken from patients with acute myeloblastic and chronic monocytic leukaemia. In many cases there was a lack of correlation between the lysozyme content of leukocytes on the one hand and that of serum and urine on the other hand. Possible causes underlying this lack of correlation are discussed.

Humans↗