Plasma exchange: an option for the treatment of life-threatening status asthmaticus in pregnancy.
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Adult↗
Biomedical subjects
H Günther
Publications and source records attributed to H Günther.
Flow cytometric DNA quantification in immunophenotyped cells as a sensitive method for determination of aneuploid multiple myeloma cells in peripheral blood stem cell harvests and bone marrow after therapy.
The simultaneous measurement of DNA content and myeloma-related antigens (B-B4 or CD38) by flow cytometry is proposed as a method for the detection of aneuploid plasma cells in peripheral blood stem cell (PBSC) harvests and in bone marrow after therapy. In 30 patients with initially detected aneuploid myeloma cells we evaluated the bone marrow after therapy and in eight of these patients 23 PBSC harvests were analyzed. In 13 of 23 PBSC harvests aneuploid myeloma cells were detectable (range: 0.02-0.63%). In the bone marrow of the 30 patients aneuploid plasma cells were detectable in all samples after chemotherapy (range: 0.12-35.70%) and after autologous PBSC transplantation in two of three patients (0.21% and 0.03%). Furthermore the relationship between diploid and aneuploid plasma cells can be evaluated. In the PBSC harvests the percentage of aneuploid plasma cells is significantly lower than that of diploid plasma cells (P=0.006). In contrast, in bone marrow the aneuploid plasma cells are predominant in most patients even after therapy (24 of 30 patients; P=0.0055). In the case of initially detected aneuploid myeloma cells, a contamination with malignant cells can be estimated with a simple flow cytometric method in PBSC harvests and in bone marrow after therapy.
Aneuploidy↗
Molecular characterization of a bovine enteric calicivirus: relationship to the Norwalk-like viruses.
Jena virus (JV) is a noncultivatable bovine enteric calicivirus associated with diarrhea in calves and was first described in Jena, Germany. The virus was serially passaged 11 times in colostrum-deprived newborn calves and caused diarrheal disease symptoms at each passage. The complete JV genome sequence was determined by using cDNA made from partially purified virus obtained from a single stool sample. JV has a positive-sense single-stranded RNA genome which is 7,338 nucleotides in length, excluding the poly(A) tail. JV genome organization is similar to that of the human Norwalk-like viruses (NLVs), with three separate open reading frames (ORFs) and a 24-nucleotide sequence motif located at the 5' terminus of the genome and at the start of ORF 2. The polyprotein (ORF 1) consists of 1,680 amino acids and has the characteristic 2C helicase, 3C protease, and 3D RNA polymerase motifs also found in the NLVs. However, comparison of the N-terminal 100 amino acids of the JV polyprotein with those of the group 1 and group 2 NLVs showed a considerable divergence in sequence. The capsid protein (ORF 2) at 519 amino acids is smaller than that of all other caliciviruses. JV ORF 2 was translated in vitro to produce a 55-kDa protein that reacted with postinfection serum but not preinfection serum. Phylogenetic studies based on partial RNA polymerase sequences indicate that within the Caliciviridae JV is most closely related to the group 1 NLVs.
Amino Acid Sequence↗
[Primary renal plasmacytoma. A case report].
We describe a patient with a primary renal plasmacytoma. In the literature available, only six clinical cases have been reported. A 64-year-old patient is presented who had the clinical signs of a renal cell carcinoma. Histological examination after nephrectomy, however, revealed a plasmacytoma. Based on this case, we discuss how one should proceed in this disease. Primary renal plasmacytoma can be tentatively diagnosed preoperatively only in the presence of paraproteinemia or Bence-Jones proteinuria, as it cannot be distinguished from other renal tumors by imaging procedures. Postoperatively, further staging procedures must rule out bone involvement (solitary myeloma or multiple myeloma). Nephrectomy is required in patients with plasmacytoma only if renal complications occur.
Carcinoma, Renal Cell↗
[Can discrimination ban guarantee equality of opportunities for disabled persons?].
Embodied in Germany's Basic Law since 1994, the discrimination ban on behalf of disabled persons--in line with similar provisions introduced in other countries--highlights the fact that disabled persons, as individuals and with their lawful rights, have become the point of departure of disability policies, as opposed to the hitherto prevailing social welfare perspective. Banning discrimination alone, though, will enable equal opportunities to be achieved only for some areas of social life; positive discriminations in favour of disabled persons are possible and necessary in addition. When it comes to integration into worklife, banning discrimination against disabled persons will achieve but little. Rather, in a market economy, employers and the general public share a common responsibility, for one, by respecting employers' rights of disposal over their jobs and, for the other, by changing present labour market conditions in favour of disabled persons, i.e., taking measures of positive discrimination.
Persons with Disabilities↗
29Si MAS solid state and 129Xe NMR of porous silica.
The condensation of surface hydroxyl groups of silica after high-temperature treatment has been studied by 29Si MAS solid state and 129Xe NMR spectroscopy. Above 500 degrees C, the number of surface hydroxyl groups is diminished and intraparticle 129Xe species, which reside in the pores of silica and rapidly exchange between pores of different size, are increasingly shielded. Chemical exchange between intraparticle xenon with xenon in interparticle voids is also enhanced upon thermal treatment of silica and can be monitored by 2D EXSY spectroscopy.
Isotopes↗
Purification and characterization of the NADH-dependent (S)-specific 3-oxobutyryl-CoA reductase from Clostridium tyrobutyricum.
An NADH-dependent (S)-specific 3-oxobutyryl-CoA reductase from Clostridium tyrobutyricum was purified 15-fold with a yield of 46%. It was homogeneous by gel electrophoresis after three chromatographic steps. The apparent molecular mass was estimated by column chromatography to be 240 kDa. SDS-gel electrophoresis revealed the presence of 33 kDa subunits. Substrates of the enzyme were ethyl and methyl 3-oxobutyrate, 3-oxobutyryl-N-acetylcysteamine thioester, and 3-oxobutyryl coenzyme A. The specific activities were 340 and 10U (mg protein)-1 for the reduction of 3-oxobutyryl coenzyme A and ethyl 3-oxobutyrate, respectively; the Michaelis constants were 300 microM and 300 mM, respectively. The identity of 12 N-terminal amino acid residues was determined. The enzyme was used in a preparative reduction of substrate, yielding ethyl (S)-3-hydroxybutyrate (> 99% enantiomeric excess).
3-Hydroxyacyl CoA Dehydrogenases↗
Purification and characterization of an (S)-3-hydroxycarboxylate oxidoreductase from Clostridium tyrobutyricum.
An NADP(+)-dependent reversible 3-hydroxycarboxylate oxidoreductase present in Clostridium tyrobutyricum has been purified. As judged by gel electrophoresis the enzyme was pure after a 940-fold enrichment by four chromatographic steps. Its molecular mass was estimated to be 40-43 kDa. The enzyme was most active at pH 4.5 in the reduction of 3-oxobutyrate. Other substrates were 3-oxovalerate, 3-oxocaproate, 3-oxoisocaproate and 4-chloro-3-oxobutyrate. Except for the latter all substrates were converted enantioselectively to (S)-3-hydroxy acids in the presence of NADPH. 4-Chloro-3-oxobutyrate was reduced to the (R)-3-hydroxy acid. The specific activity of the enzyme was about 1400 mumol min-1 mg-1 protein for the reduction of 3-oxobutyrate at pH 5.0. The Michaelis constant (Km) values for 3-oxobutyrate, 3-oxovalerate and 3-oxocaproate were determined to be 0.22, 1.6 and 3.0 mM, respectively. The Km values for dehydrogenation of (S)-3-hydroxybutyrate, (S)-3-hydroxyvalerate and (S)-3-hydroxycaproate were found to be 2.6, 1.1 and 5.2 mM, respectively. The identity of 43 of the first 45 N-terminal amino acid residues has been determined. So far such enzyme activities have been described in eucaryotes only.
3-Hydroxybutyric Acid↗
Outpatient coronary angiography--safety and feasibility.
The feasibility and safety of outpatient coronary angiography were studied in 2,106 patients. Patients were discharged with a pressure dressing 2 h after the angiographic study. No complications occurred in 99.53% of all patients. Severe complications were seen in 10 patients (0.47%). 9 patients (0.43%) had to be admitted to hospital, either for immediate treatment (4 patients) or due to complications (5 patients). Our results revealed a very low complication rate for outpatient coronary angiography. The number and severity of complications following coronary angiography did not differ significantly between inpatients and outpatients. Therefore, outpatient coronary angiography can be considered as a safe and feasible diagnostic method which may help cut health care costs.
Adult↗
Fermenter cultivation of intestinal spirochaetes.
We cultivated 6 strains of intestinal spirochaetes in a laboratory fermenter under constant conditions (medium, physical-chemical parameters) in a permanent gas flow. It has been possible to demonstrate that the application of the fermenter technique for the cultivation of spirochaetes is a suitable method which can be easily standardized.
Bacteriological Techniques↗
[Holistic anthropology in oncology].
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Anthropology↗
[Practice observations of cross protection between bovine and porcine herpesviruses].
In many cattle herds in Lower Saxony the serologic IBR/IPV = BHV-1-status is known because since 1988 the bovine herpes virus infection (BHV 1), the infectious rhinotracheitis and pustular vulvovaginitis (IBR/IPV) are being fought. Through extensive investigations in five districts in the Weser-Ems area, it was found that cows infected by bovine herpes virus (BHV 1) are also protected to a high degree against infection by porcine herpes suis virus (SHV 1). An interpretation of the observed cross protection is not yet possible; further research is necessary.
Animals↗
[Histological studies of the occurrence of organisms shaped like Campylobacter in the abomasum of calves].
The mucosa of the abomasum is a strongly acidic and therefore germ poor, but not entirely germfree environment. Only in isolated areas of the fundic glands zone were straight rods in the foveolas, and even more seldom spiral shaped bacteria could be encountered. They had no relation to the inflammatory infiltrates. On the other hand, Campylobacter-like organisms were more often observed in the pyloric region and, preferably, in a narrow zone following the cutaneous mucosa of the omasum. These organisms appeared to have a different size (1.3 to 2.4 microns length and 0.4 to 0.8 microns width) and they occurred deep inside the glands in an almost pure fashion. Whereas they caused no visible reaction of the tissue in the narrow cardiac zone, their occurrence in the pyloric region was several times connected with neutrophilic-rich infiltration. It ought to be tested, whether Helicobacter are among these Campylobacter-like organisms, which cause a disease leading up to ulcerations in calves similar to gastritis B in humans.
Abomasum↗
[Biological monitoring in the area around a steel mill].
On the basis of measurements of dust sediment samples, carried out from 1984 to 1988 in the environment of trace element emitting plants and industrial centers of the former GDR, the exposure of the people in the region of the ferrometallurgical plant Brandenburg (with 12 Siemens Martin furnaces) had been investigated. Samples of hair, urine and blood of 170 men and children had been taken for this investigation. For a correct assessment of the exposition we have also measured the dust in flats. In comparison to persons from clean regions higher concentration level of Cu and Mn in hair, Cd in urine and Pb in blood were found in the population group living near to the emittent. But these concentration levels have not yet reached an acute danger of inhabitants of the investigated industrial centre. The limited increase of heavy metal concentration is also a consequence of the information of the inhabitants.
Adult↗
[The emission of heavy metals with dust suspensions at 33 selected measuring points in the former East Germany (time period 1983-1988)].
2200 weekly samples of airborne particulate matter from 33 measuring sites have been obtained by 12 district institutes of hygiene (Gera, Chemnitz, Leipzig, Dresden, Erfurt, Frankfurt, Magdeburg, Neubrandenburg, Rostock, Cottbus, and Berlin). After pressure digestion of the impacted membrane filters the concentrations of 15 elements (Ca, Mg, Sr, Ba, Be, Fe, Al, Ti, Cr, Mn, V, Zn, Cu, Pb, and Cd) were analysed by ICP-Spectrometry. The Immissions were calculated using the air sample volumes. Measuring points with very high immissions are: steel plant Riesa, district Dresden (nonferrous and ferrous metals), Drübeck, near to the copper plant Ilsenburg (nonferrous metals), Lead paint plant Ohrdruf, district Erfurt (highest Pb-Immission: 3137 ng/m3), Special glass plant Weisswasser, district Cottbus (Ba, Pb and Cd), Leipzig (dust and all other elements). Four groups of measuring site with different characteristic types of immissions could be distinguished by means of factor and cluster analysis. The separation of these groups by discriminant analysis was significant (P greater than 0.95). The seasonal trends showed maximal values in winter and minimal values in summer.
Air Pollutants↗
[The cancer patient in science and society].
The 2nd Dresden hematonocological meeting, organized by the Department of hematology and oncology of the Medical Academy "Carl Gustav Carus" and by the Tumorzentrum Dresden, focused ethical and anthropological topics. Death and dying, care and health prevention in modern oncology as well as the broad field of supportive care were discussed. The goal of the meeting was to find long time concepts of a patient oriented medical care in cancer patients. This will only be possible in interdisciplinary structures including philosophers, theologians, clinicians and general practitioners. The rapid progress of medical development in Eastern Germany must not forget this goal.
Ethics, Medical↗
[Tuberous sclerosis: family manifestations].
The authors report on 6 sisters and brothers with Bourneville's disease (tuberous sclerosis). The article describes the different morphologic manifestations of the illness in the patients and places them in relation with the generally assumed principal signs with special reference to the new imaging techniques such as cranial computed tomography and magnetic resonance imaging. These methods reveal pathognomonic patterns already very early, in association with specific clinical changes. Tuberous sclerosis must be excluded even in apparently healthy relatives by an exact diagnosis. Computed tomography is an absolutely indispensable indicator in this regard, since it is a highly sensitive method.
Brain↗
Hemodilution and myocardial oxygen supply. The influence of fluosol-DA.
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Animals↗