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Biomedical subjects

H Fukasawa

Publications and source records attributed to H Fukasawa.

At least 109 records · Page 6Linked to original sources
PubMed1984

[Ferritin levels of ascites and the contents of cystic ovarian tumors--possibilities of ferritin production and secretion by malignant tumor cells].

In this paper, we presented an interesting case with a cystic ovarian tumor, which consisted of the mucinous cystadenoma and the mucinous cystadenocarcinoma separated by a partition. The determination of ferritin concentrations in the both displayed ferritin levels of 87.5 ng/ml in the former and 14,812.0 ng/ml in latter respectively. This fact suggested the production of ferritin by the malignant tumor cells. To clarify this possibility, measurement of ferritin levels in the content of cystic ovarian tumors in fifteen cases and in the ascites of various origin in sixty-five cases was done. This study also supported a possibility that malignant tumor cells produce and/or secrete much more ferritin into the body fluid than benign tumor cells.

Ascitic Fluid↗
PubMed1984

[Angiographical extravasation in the intracranial hemorrhage due to cerebrovascular moyamoya disease--autopsy study].

A 53-year-old, non-hypertensive farmer, who had sudden attack of severe headache, was transferred to our clinic. He presented comatous state and tetraparesis without extraocular movements nor reactive pupils to light. CT scan, 7 hours after the ictus showed intracerebral hematoma in the right temporo-parietal region with ventricular extension. The following bilateral carotid angiograms established the diagnosis of the intracerebral hemorrhage due to cerebrovascular moyamoya disease. In angiograms of the affected side, irregular spotty stains spread from the periphei of the right posterior choroidal artery was delineated. The repeated CT scan after that indicated increment of hematoma. Fifty-three hours from the ictus, the patient died and an autopsy study was performed. After the fixation, the coronal brain section was made, and the careful observation of them elucidated the formation of an organized dissecting aneurysm in the angiographically extravasated vessel. About seven hundreds of serial specimen, 4 micron in thickness, was then investigated adjacent to the aneurysm. The organized dissecting aneurysm seemed to initiate from the branch of it, where marked fraying and undulation of the fibroelastic intima and internal elastic laminae were observed. The concavity toward the true lumen was completely disrupted and communicated to the extravascular space. As a result, the continuous part of it obstructed the lumen of the branch. These findings suggested the newly-developed dissection and it seemed to correspond to the angiographical extravasated points.

Arsenic↗
PubMed1982

Isolation and partial characterization of two distinct DNA topoisomerases from cauliflower inflorescence.

DNA topoisomerases which remove superhelical turns in closed circular DNA have been isolated from cauliflower inflorescences using polyethylene glycol fractionation, ammonium sulfate precipitation, and column chromatography on CM-Sephadex or CM-cellulose and DNA-cellulose. Two distinct enzymes, topoisomerase-I and ATP-dependent topoisomerase, were separated clearly by CM-Sephadex or CM-cellulose, and partially characterized using agarose gel electrophoresis with plasmid pBR322 DNA. Topoisomerase-I acts like other eucaryotic DNA topoisomerases in the absence of ATP, is stimulated by spermidine and inhibited by EDTA. The ATP-dependent topoisomerase acts like topoisomerase-I only in the presence of ATP in the reaction medium, is inhibited by spermidine and EDTA, and does not introduce supertwists into closed duplex DNA or produce catenate aggregates under the present reaction conditions.

Adenosine Triphosphate↗
PubMed1982

A generalization of the atherosclerotic index and its application.

A generalized atherosclerotic index with n types of lesions I(n) was defined in the form of an inner product of the profile vector alpha and the weight vector beta as: I(n) = 100 (alpha, beta), alpha = 1/A(alpha 0, alpha 1, ..., alpha n) and beta = 1/Wn(0, W1, ..., Wn), where A is the total area examined, alpha i(0 less than or equal to i less than or equal to n) is the area of the i-the type of lesion, the subscript 0 refers to the normal portion, and Wi is the weight to be given to the corresponding lesions, with only the restriction 0 less than or equal to W1 less than or equal to ... less than or equal to Wn. It was further extended to a composite, index of m arterial systems in matrix multiplication from as I(m,n) = 100 PQR, P and R being weight matrices and Q an observation matrix. As an example of its application, results obtained from our autopsy series were shown. In a total of 288 cases classified into four disease groups, the cerebral infarction group was highest in the severity of aortic atherosclerosis both in the customary index and in a modified index in which relatively small weight was given to the complicated lesion. The order of the other three groups was more clearly represented in the latter index than in the former.

Adult↗
PubMed1982

[Incidental meningioma].

Intracranial tumors are accidentally discovered during operations for neurosurgical diseases, such as head trauma or cerebral aneurysm. Furthermore, in the autopsy specimen, intracranial tumors which had not been recognized before the patient's life are sometimes found incidentally. Those tumors are; meningioma, glioma, pituitary adenoma, and so forth. Among these instances, meningioma seems to be the most frequent. In this paper, the authors discussed five cases of asymptomatic meningiomas found unexpectedly, referred as "Incidental Meningioma." Incidental meningioma listed up here are five out of 38 clinical cases with intracranial meningioma which authors experienced during recent twelve years in our hospital. In 480 autopsy cases in our hospital in the same period, there was only one case with which incidental intracranial tumor was detected. This incidental tumor was index-finger tip sized parasagittal meningioma. This was not included in this series. These 5 clinical cases were composed of all women ranging in age from 48 to 72 years. Three of them were discovered during aneurysm surgery, the other one was found during the evacuation of acute subdural hematoma. The remaining one case (Case 5) was demonstrated intracranial meningioma unexpectedly by CT scanning. The size of those tumors were ranged from 7 to 20 mm in diameter except Case 5. In Case 5, tumor size was about 30x40 mm in diameter. The localization of those tumors were; three convexity, one pterion, and one falx meningiomas. Positive findings referable to those tumors at ancillary neuroradiological examinations, were shown only in one case (Case 2) with the exception of Case 5 retrospectively. That was abnormal calcified shadow in frontal bone at the right side in Case 2. The histological nature of them was various, but psammomatous type belonging to transitional meningioma was worthy to note, because such a type was relatively rare in our total cases. Incidental tumors seemed to be relatively rare in cases with cerebral aneurysm. In our series comprising 747 operated cases for cerebral aneurysm, there were 5 cases with incidental tumor (0.67%). Three of these 5 cases were incidental meningioma which were listed up in the paper. In all cases with incidental meningioma, except for Case 5, there were no definite feeding arteries, and they could be easily extirpated, with uneventful recovery.

Aged↗
PubMed1981

Template-specific inhibitor for DNA polymerase isolated from cauliflower inflorescence.

A novel inhibitory factor which greatly inhibits DNA polymerase activity was isolated from the apical portion of the cauliflower inflorescence during purification of DNA polymerases. It can be adsorbed on a DEAE-cellulose column, but not on CM-Sephadex or DNA-cellulose. The factor exclusively inhibits the incorporation of [3H]dTTP into DNA when poly(rA, dT10) is used as the template primer, but not when activated DNA, heat-denatured DNA or native DNA is used as a template. The concentration of the factor in the reaction medium required for 50% inhibition is approx. 8 microgram/ml. The factor is heat-stable, is inactivated by trypsin, and has a maximum ultraviolet absorption at 278 nm. The molecular weight was estimated as 2500-3000 by Sephadex gel chromatography.

Chromatography, DEAE-Cellulose↗
PubMed1981

Diffuse white-matter disease in the geriatric population. A clinical, neuropathological, and CT study.

CT findings in 6 autopsy cases of subcortical arteriosclerotic encephalopathy (SAE) are reported. A diffuse area of nonhomogeneous decreased density was observed in the deep white matter of both cerebral hemispheres, together with moderate dilatation of the lateral ventricles and ragged margins. The most characteristic pathological findings at autopsy were a diffuse area of incomplete infarction containing multiple small infarcts as well as cyst formation and marked stenotic atherosclerotic changes in the medullary arteries. Clinical features included patchy mental lapses, frontal-lobe syndromes, minor motor signs, and hypertension. The authors feel that SAE or a similar disease might occur in most cases of multi-infarct dementia.

Aged↗
PubMed1980

Characterization of two DNA polymerases from cauliflower inflorescence.

Two distinct DNA polymerases, A and B, isolated from a rapidly growing apical tissue of cauliflower (Brassica oleracea var. botrytis) inflorescence have been characterized, and compared with DNA polymerases, alpha and beta, from mouse myeloma. Polymerase-A bears a strong resemblance to polymerase-alpha from mammalian cells in all properties examined. The character of polymerase-B is also quite similar to polymerase-beta of mammalian cells in chromatographic elution properties, template-primer utilization, sensitivity to inhibitors, response to KCl or KPi concentration, and other requirements for maximal activity, although it has a higher molecular weight (approx. 78,000) even in the presence of 0.25 M KCl that polymerase-beta (mol. wt. less than or equal to 50,000) of mammalian cells. This type of DNA polymerase has not been reported to exist in the plant system.

Animals↗
PubMed1979

Properties of common wheat ferredoxin, and a comparison with ferredoxins from related species of triticum and aegilops.

Wheat ferredoxin was purified from the leaves of common wheat (Triticum aestivum). The absorption spectrum showed maxima at 465, 425, 332, and 278 nm. The absorbance ratio, A425 nm/A278 nm was 0.49, and the millimolar extinction coefficient at 425 nm was 10.8 mM-1. cm-1. The amino acid composition was determined to be Lys5, His2, Arg1, Asp11, Thr5, Ser7, Glu18, Pro5, Gly6, Ala7, Cys5, Val7, Met1, Ile4, Leu7, Tyr4, Phe1, and Trp1. The total number of amino acid residues was 97. The molecular weight was calculated from the amino acid composition to be 10,829, including iron and sulfur atoms. This value was confirmed by other methods, which were based on the contents of non-heme iron and of terminal amino acid. The N-terminal amino acid was alanine, and the C-terminal amino acid sequence was -Glu-Leu-Thr-AlaCOOH. Comparative studies were performed between T. aestivum ferredoxin and ferredoxins isolated from closely related species; these were T. aegilopoides, T. durum, Ae. squarrosa, and Ae. ovata. No significant differences in the properties of these ferredoxins were detected. It was also shown that these ferredoxins are immunologically homologous. It is, therefore, likely that one molecular species of ferredoxin is distributed through two genera of Triticum and Aegilops.

Amino Acids↗