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Biomedical subjects

H Fujimori

Publications and source records attributed to H Fujimori.

At least 73 records · Page 4Linked to original sources
PubMed1990

Direct injection of blood samples into a high-performance liquid chromatographic adenine analyser to measure adenine, adenosine, and the adenine nucleotides with fluorescence detection.

Adenine (Ade), adenosine (Ado) and its nucleotides such as AMP, cAMP, ADP and ATP in blood or plasma were determined by a high-performance liquid chromatographic (HPLC) adenine analyser with fluorescence detection. In order to inject samples directly into the HPLC system without pretreatment except dilution, the analyser consisted of two systems each, having three columns (pre-, mini- and analytical). A precolumn with an inlet filter of pore size 40 microns was common to both systems and packed with Butyl-Toyopearl 650-M to remove hydrophobic compounds and blood cell membranes. In the system for analysis of the nucleotides, a mini-column of Hitachi anion-exchange gel 3013-N was used for adsorbing AMP, cAMP, ADP and ATP. The adsorbed nucleotides were separated by the Hitachi gel 3013-N analytical column. In the other system for analysis of Ado and Ade, they were adsorbed on a Develosil ODS-5 mini-column and separated by an Asahipak GS-320H size-exclusion analytical column. The adenine compounds in each eluate were derivatized on-line in a 15-m reaction coil at 115 degrees C with bromoacetaldehyde as the fluorescent reagent in each mobile phase for the analytical column, and detected by spectrofluorimetry. ATP, ADP and AMP were accurately determined by the direct injection of hamster, rat and human whole blood. Authentic Ade and Ado were well separated and Ado in human plasma was determined, but it was difficult to determine it in rat plasma owing to interference from an unknown compound.

Adenine↗
PubMed1990

Measurement of adenine nucleotide levels with an adenine analyser as an index of freshness of porgy.

In a prototype of an adenine analyser, adenosine and adenine nucleotides were derivatized with a fluorescent reagent, bromoacetaldehyde, after separation on a Hitachi gel No. 3012-N column by high-performance liquid chromatography. The previous analyser was improved by using a shorter reaction coil and by introduction of a Hitachi gel No. 3013-N with 5-microns particles of porous polystyrene-divinylbenzene, and applied to estimate the freshness of porgy. Total amounts of ATP, ADP and AMP in an isolated muscle just after death gradually decreased to 60% of the original amount after 5 h, and the ATP content rapidly decreased to 20% after 1 h. A good correlation was found between the levels of total adenine compounds and the energy charge values obtained from nineteen porgies frozen at a prerigour state. On the other hand, there existed no relationship between total adenine levels and the K values, which were indices for estimating freshness of fish. The analyser will be useful to evaluate the freshness of tissues and cells based on the higher contents of total adenine compounds, especially ATP.

Adenine Nucleotides↗
PubMed1990

Effects of arginine derivatives on soluble guanylate cyclase from neuroblastoma N1E 115 cells.

The effects of L-arginine (Arg) derivatives on soluble guanylate cyclase from neuroblastoma N1E 115 cells were examined. The Arg derivatives were modified at the -NH2, -COOH, C alpha-proton or guanidino group of Arg. Among the synthesized derivatives, eight compounds, i.e. the 5-(dimethylamino)-1-naphthalenesulfonyl (DNS) ones, especially N-cyclohexyl-2-(N-DNSamino)-5-guanidino-2-methylvaleramide and 1-[2-(N-DNSamino)-2-(2-imino-1,2,3,4,5,6-hexahydropyrimidin- 4-yl)acetyl]- piperidine, were found to inhibit the activity of crude guanylate cyclase in the 105,000 g supernatant fraction of the cell homogenate. The enzyme, partially purified by a column of Chelex 100 Na+, was also inhibited by these eight compounds. The mode of the inhibition was competitive. The Ki values were in the range of 2-8 microM for the enzyme in the 105,000 g supernatant fraction and 3-16 microM for the partially purified enzyme, in the presence of Mg2+ as a metal cofactor. In contrast, a new derivative, methyl 2-amino-5-guanidinovalerate (M Arg ME), as well as the Arg methyl ester (Arg ME) and Arg; were found to enhance the activity of the partially purified guanylate cyclase; KA values of M Arg ME, Arg ME and Arg were approximately 9, 4 and 3 microM respectively. From these results, the free guanidino group including 2-imino-1,2,3,4,5,6-hexahydropyrimidin-4-yl or 2-imino-1,2,3,4,5,6-hexahydropyrimidin-5-yl and modification of the --NH2 residue with a hydrophobic group such as DNS seemed to be essential for inhibition of the guanylate cyclase; however, the guanidino and --NH2 residue of Arg should be free for activation by these Arg derivatives.

Arginine↗
PubMed1989

[The clinical course of chronic methamphetamine psychoses].

3 cases with chronic methamphetamine psychoses (MAP-P) were studied from the clinical psychiatric point of view and discussed: 1.) The average time of occurrence of psychotic symptoms in our 3 cases after beginning of MAP abuse was two or three years. 2.) Although the psychotic states (often schizophrenia-like) were stable for a short time, the symptoms recurred promptly after reinjection of a small amount of MAP, unspecific stress or drinking sake (flashback phenomenon). The recurrence may happen even after several years. 3.) In cases of chronic MAP-P disturbances of consciousness are rare. Chronic process of MAP-P cases may show acute transient disturbances of consciousness (flashback phenomenon). 4.) The change of affects and behaviour ranges between irritable and indifferent attitudes. While there are close connections between the disturbance of feeling and change of personality (Wesensänderung), it is very difficult to decide whether premorbid character traits are dominant or there is a change of personality or emotional disturbance. 5.) About acute psychic symptoms which are responsible for recurrence of MAP-P, MAP-abusers make the experience that their intimate personal affairs are overlooked and become public (uncovered defensive experiences). In chronic cases of MAP-P we often meet patients with hypertrophic self-feeling or delusion of grandeur predominant in the symptomatology. 6.) In our cases sensations were amalgamated into each other and there were hallucinatory experiences without clear division between them. 7.) In chronic cases the auditory hallucinations criticized or censured the patients for their behaviour. Thought echoing (Gedankenlautwerden) was found. We found signs of mind reading (Gedankenausbreitung).

Adult↗
PubMed1989

[Performance evaluation of X-ray computed tomography "TCT-700 S". 2].

UNLABELLED: The performance of the rotate/rotate CT scanner TCT-700 S (Toshiba) was evaluated on the basis of the item entitled "User's initial acceptance tests (phase II)" set forth in "Standard of performance evaluation for x-ray computed tomography (second recommendation)" proposed by Takenaka et al. (1982). This second report in the series describes measurements of exposure and the relation between exposure and such other performance aspects as noise and contrast resolution. The exposure-performance recommendation proposes measurements of average exposure in the scanning field and a radial exposure profile. RESULTS: 1. In the case of multiple scanning, average exposures in the field were uniform because all surfaces of the perpendicular object were oriented toward the incidence of the x-ray beam. At a minimum scanning speed of 1.8 second, however, a discrepancy of 3 times occurred in exposures observed reciprocally from the left and right sides of the object. This phenomenon indicates that only one-half of the object surface is oriented toward the x-ray beam and that the x-ray tube and detectors rotate in mutually opposite directions. In the case of multiple scannings, when slice thicknesses were 10 mm, 5 mm and 2 mm, average exposures were almost uniform. But with a slice thickness of 1 mm, average exposure was twice as great. This measurement indicates inadequate collimation in the case of a 1 mm slice thickness. These average exposures were roughly proportional to the mAs (product of tube current and scan speed) in the range between a minimum of 100 mAs and a maximum of 1200 mAs. 2. Noise increased in proportion to decrease of average exposure.(ABSTRACT TRUNCATED AT 250 WORDS)

Radiation Dosage↗
PubMed1989

[Performance evaluation of the X-ray computed tomography "TCT-700 S" (Part 1)].

The performance of the Rotate/Rotate CT scanner "TCT-700 S" (Toshiba) was evaluated on the basis of the item entitled "User's initial acceptance tests (phase II)," set forth in "Standard of performance evaluation for X-ray computed tomography (second recommendation)" proposed by Takenaka et al. (1982). This report describes measurements for noise, contrast scale, spatial resolution, and high- and low-contrast resolution. Recommended phantoms were used to test performance. Results 1. Noise edepended on slice thickness and mAs. It increased as slice thickness decreased. No matter what the slice thickness, noise was great when mAs settings were small. Furthermore, short scan time caused considerable noise for each slice thickness. Reduction of mAs setting caused remarkable increased in noise when slice thickness were 1 mm or 2 mm Under routine examination, when thickness was 5 mm, at 120 kV, with an mAs setting of 440, scan field S(diameter 240 mm), and convolution filter FC-2, noise was 0.31%. When slice thickness was 10 mm at 120kv, 800mAs, with a scan field S (diameter 240 mm), and convolution filter FC-2, contrast scale was 1.84 x 10(-4) (cm-1/CT number). 2. When slice thickness was 5 mm, at 120 kV, with a mAs setting of 1200, scan field S(diameter 240 mm), and a convolution filter of FC-2, spatial resolution was 0.5LP/mm. 3. When slice thickness was 5 mm, at 120kV, with a mAs setting of 1200, scan field S (diameter 240 mm), and convolution filter of FC-2, minimum high-contrast resolution was 0.5 mm.(ABSTRACT TRUNCATED AT 250 WORDS)

Evaluation Studies as Topic↗
PubMed1987

Analyses of adenosine and adenine nucleotides in biological materials by fluorescence reaction-high-performance liquid chromatography.

A previous method of determination of adenine compounds by high-performance liquid chromatography, using bromoacetaldehyde as a fluorescent reagent and a column of Hitachi gel No. 3012-N, was improved and extended to biological materials, especially to measure enzyme activities. A column packed with finer beads, Hitachi gel No. 3013-N, was found to be better than that of No. 3012-N, judging from the analysis time and resolution. ADP, from the hydrolysis of ATP by Na, K-ATPase, was determined quantitatively, and the enzyme activity was inhibited with ouabain. cAMP obtained from ATP by reaction with adenylate cyclase was also determined in the presence of various concentrations of L-epinephrine or sodium fluoride. The ATP levels in human blood were determined, and the cellular levels of ATP and ADP in neuroblastoma N1E 115 were examined as a function of cell growth.

Adenine↗
PubMed1987

[Delusions and society in Japan and China from a transcultural-psychiatric viewpoint].

The relationship between contents of schizophrenic delusions and sociocultural background in the modern society of Japan and China were studied from transcultural psychiatric aspects. The data of this study were derived from the public mental hospitals in Tokyo and Shanghai in a similar size; of 186 cases (88 cases of male, 98 cases of female) of the first admission in Tokyo Metropolitan Matsuzawa Hospital during 1981-1983, 112 cases with delusions (53 cases of male, 59 cases of female) were selected, and of 200 cases (112 cases of male, 88 cases of female) of the first admission in Shanghai Psychiatric Hospital in 1983, 129 cases with delusions (70 cases of male, 59 cases of female) were chosen. The incidence of delusions of physical persecution and grandeur was relatively high in patients of both hospitals in Tokyo and Shanghai, while the incidence of delusions with hypochondria and guilt was low in both hospitals. Only the incidence of delusion of poisoning was significantly higher in Shanghai than in Tokyo (mean 2 = 12.97, p less than 0.001). After the World War II, the patriarchally oriented family system was abolished in Japan which caused shifting the system from a large family to a nuclear family. In China where the property (land) and daily life were closely connected the close human relationship among generations had important values in relation to the labor power within the frame of a large family. It is believed that occurrence of the delusion of poisoning might be a reflection of the disturbed human relationship within the family member in dining which should be helpful for making further understanding and reliance. It's occurrence conflict and struggle in the community of the outside of the family. At present there is a marked difference between Chinese and Japanese in their structure of consciousness. The former places high value and meaningfulness on the participation to the group and seeks protection and safety of individuals. The latter reveals a less strong tie with the traditional conformity and dependency to the mass due to the conformation of the nuclear family. It can not be ignored the possible effect of the close horizontal interaction and cautious human relationships among neighbours with fear of rumor and watching in the Chinese community on the occurrence of the delusion of persecution. After the World War II, abolishment of the principle of vertical control system and introduction of multiple value system together with loss of authority had taken place in Japan.(ABSTRACT TRUNCATED AT 400 WORDS)

Adult↗
PubMed1986

Purification and partial characterization of cat colon and submandibular gland kallikreins.

Kallikreins from cat colon and submandibular gland have been purified by acetone fractionation of tissue extracts, DEAE-Sephacel ion-exchange chromatography, rho-aminobenzamidine Sepharose 4B affinity chromatography and gel filtration on Sephadex G-75. They were of similar M.W., approximately 40,000, and each comprised five forms by isoelectric-focusing (pI 4.1-4.8). Both enzymes were potent kininogenases and exhibited similar specificities with synthetic ester and amide substrates. They were susceptible to a range of protease inhibitors. Surprisingly, neither was sensitive to aprotinin yet both were partially inhibited by soya-bean trypsin inhibitor. They were indistinguishable in our immunological tests. An acidic esterase (pI 2.2-3.5) of M.W. 120,000 was isolated from cat stomach by the same procedure. While it exhibited weak immunologic similarity to cat submandibular gland kallikrein, it had negligible kininogenase activity and different substrate and inhibitor specificities to the two kallikreins. It is concluded that similar tissue kallikreins are present in the colon and submandibular gland of the cat but are distinct from this cat stomach esterase.

Animals↗
PubMed1986

Purification and partial characterization of cat pancreatic and urinary kallikreins--comparison with other cat tissue kallikreins and related proteases.

Kallikreins have been purified from cat pancreas and urine by methods similar to those described previously for cat colon and submandibular gland kallikreins. The pancreatic kallikrein (M.W. 41,200, pI 4.75) was similar to the urinary kallikrein (M.W. 34,300 pI 4.35-4.70) in pH optimum, substrate specificity and inhibition profile. Both enzymes were potent kininogenases and immunologically similar. These enzymes closely resembled the kallikreins from cat colon and submandibular glands. A trypsin (M.W. 18,800) was isolated from cat pancreas and shown to be distinct from the group of kallikreins in all parameters tested. We attempted purification of cat renal kallikrein, but were unable to isolate any such enzyme. The major acidic esterase of cat kidney cortex (M.W. 59,000, pI 4.91) was purified and was distinct from both the cat tissue kallikreins and trypsin. The origin of cat urinary kallikrein remains unclear, but in the light of our findings, it may result from renal filtration of blood-borne tissue kallikreins rather than from intrarenal synthesis.

Animals↗
PubMed1986

[Suicide by schizophrenic patients in psychiatric hospitals].

A detailed investigation was carried out on the schizophrenics who committed suicide at the Tokyo Metropolitan Hospital Matsuzawa during the last 30 years. Of these 58 schizophrenics, 52 cases (male 25, female 27) were studied in this report. The mean age of suicide patients was 38.6 years (male: 34.3 years, female: 42.6 years); in males the suicide occurred between 20 and 39 years (80%), while females showed a wider distribution with two peaks in the 25 to 34 years group (26%) and the 40 to 54 years group (41%). 88% (22/25) of males were unmarried, whereas only 33% (9/27) of the women were unmarried. Suicide methods were comprised of hanging (51.9%-27 cases), being run over by a streetcar (28.8%-15 cases), drowning (6 cases), jumping off a high building (3 cases) and intoxication by creosol liquid soap (1 case). In case of male patients hanging was most frequent (72%), followed by jumping into a streetcar to get run over (24%). Likewise, hanging and jumping into a streetcar were most frequent in females (33.3%, respectively), followed by drowning (22.2%), a method selected by females only. From 1951 to 1960 (right before and after the introduction of psychopharmacotherapy) hanging ranked first, but the suicide methods were shifted to the jumping into a streetcar and drowning after installation of the Open-Door-System (around the years 1961-1970). During the last 10 years (from 1971 to 1980) suicide by jumping from a high building increased. Suicide committed in the open ward occurred 9 times only (17.3%); it was, therefore, a much rarer occurrence than had been expected. This fact may show that such an open ward system would be associated with a relatively low risk of suicide. After introduction of psychiatric pharmacotherapy and as social rehabilitation continued, the incidence was relatively stable and no increase was found in our data. Only 4 patients (7.7%) left suicide notes, which made it difficult to draw a clear conclusion on the motivations. Twenty-six cases (50%) died by the first attempted suicide, but in 13 cases (25%) suicide was finally accomplished after the second trial or more. Women performed several repeated suicide attempts (37%), whereas the male patients died at the first or second attempt (88%). Hospitalization lasted on an average for 5 years and 8 months (male: 3 years and 8 months, female: 7 years and 6 months); the shortest stay was 2 days and the longest was 25 years and 11 months.(ABSTRACT TRUNCATED AT 400 WORDS)

Adult↗