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Biomedical subjects

H Fujii

Publications and source records attributed to H Fujii.

At least 1,225 records · Page 68Linked to original sources
PubMed1980

Membrane abnormality in red blood cells with weak type B expression.

The mechanisms of unusually weak blood group (A and B) expressions are not yet well understood. We examined properties of blood group galactosyltransferase (B-enzyme) and characteristics of red cell membrane components obtained from family members with A2Bm character. B-enzyme activity of the A1Bm plasma is in normal range, and kinetic properties (i.e., Km for UDP-Gal, Km for 2'-fucosyllactose, and pH optima) of B-enzyme from the A1Bm subjects are identical to that of normal B-enzyme. When A1Bm red cell were incubated with UDP-Gal and B-enzyme, the cells became strongly agglutinable with anti-B. When A1Bm membranes were incubated with B-enzyme or A-enzyme (i.e., blood group N-acetylgalactosaminyltransferase) and the appropriately labeled nucleotide sugar (UDP-Gal3H for B-enzyme and UDP-GalNAc3H for A-enzyme), significant incorporation of the sugar was observed. The amounts of the sugar incorporated into A1Bm membranes were about 40%-50% of that incorporated into O membranes at saturation, indicating that about one-half of H-sites remained unglycosylated in A1Bm red cells. Examination of radioactive components by isoelectric focussing revealed that the labeled components of A1Bm membranes were distinctively different from that of O membranes. Therefore, one can conclude that the weak B expression is not due to direct mutation of ABO locus, but due to a secondary consequence of genetic abnormality of a membrane component (or components) associated with blood group substances.

ABO Blood-Group System↗
PubMed1979

Electrophoretic and kinetic studies of a mutant red cell pyrimidine 5'-nucleotidase.

Human red cell pyrimidine 5'-nucleotidase (P5N) was studied by partial purification and condensation in a patient with an extremely low red cell P5N activity and chronic hemolytic anemia. The residual P5N in the red cell of the patient was characterized by an increased Michaelis constant for cytidine 5'-monophosphate, a marked shift of the pH optimum to the acidic side, normal electrophoretic mobility and normal heat stability. These data indicate that, in this patient, severe enzyme deficiency is caused by a structural gene mutation. This variant is clearly distinguished from a previously reported case and it is designated P5N Kagoshima.

Anemia, Hemolytic, Congenital↗
PubMed1979

A role of red cell pyrimidine 5'-nucleotidase in experimental lead poisoning.

Intravenous administration of lead acetate to rabbits for 10 weeks at 2 week intervals resulted in significantly elevated blood lead levels, slight anemia with marked microspherocytosis and moderate basophilic stippling, and marked depression of red cell delta-aminolevulinic acid (ALA) dehydratase activity. However the decrease in red cell pyrimidine 5'-nucleotidase (P5N) activity was slight when compared to the red cell P5N activity of comparable reticulocyte-rich blood, and intracellular accumulation of pyrimidine nucleotides could not be demonstrated. In the in vitro inhibition test the same degree of inhibition of red cell P5N activity seen in hereditary red cell P5N deficiency was obtained by using a lead concentration 200--400 times higher than the lead levels detected in human plumbism. Most importantly, there were no differences in the lead-induced inhibition of human and rabbit red cell P5N. From the results of the in vitro inhibition test, lead-induced red cell P5N deficiency appears to be one of several pathogenic mechanisms in chronic lead exposure associated with the accumulation of lead in bone marrow. A decrease in rec cell P5N activity could not be demonstrated despite the marked depression in red cell ALA dehydratase activity, and slight anemia with marked microspherocytosis and moderate basophilic stippling in this experiment. These results suggest that lead affects red cells at multiple metabolic loci.

Anemia↗
PubMed1979

Effects of alpha- and beta-D-glucose on germination of spores of Bacillus megaterium QM B1551.

The effects of D-glucose anomers on the germination of dormant spores of Bacillus megaterium QM B1551 were studied, alpha-D-Glucose (1 mM) slightly initiated the germination of the dormant spores during 10 min incubation at 37 degrees C, while about 60% of the dormant spores became germinated with beta-D-glucose (1 mM) in the same conditions. From the above observations and the finding that only a trace amount of alpha- or beta-D-glucose may bind with the dormant spores, it is speculated that the beta-D-glucose-stereospecific receptor site for the germination exists on the surface of the dormant spores of the bacillus.

Bacillus megaterium↗
PubMed1979

Condylar displacements related to dynamic and static recordings of the comfortable zone.

In a previous study it was found that the distance between the borders of the comfortable zone was considerably wider when the zone was recorded statically rather than dynamically. However, it was not reported how condylar displacements may be related to the zonal borders. Therefore, an attempt is made in the present study to translate zonal border recordings into the equivalent amount of condylar rotation. Using a dental articulator, it was found that the condyles rotated 4.96 degrees, when the comfortable zone was recorded statically, and 2.50 degrees, when the zone was recorded dynamically. The difference, 2.46 degrees, was statistically significant (P less than 0.001).

Dental Articulators↗
PubMed1979

Red cell enzyme activities and properties of mutant pyruvate kinase after long-term storage of red cells in liquid nitrogen.

After 8 months of storage of normal red cells in liquid nitrogen, red cell enzyme activities were essentially unchanged, except for triose phosphate isomerase which was reduced by about half. Mutant pyruvate kinase (PK), PK Tokyo I, showed no changes in kinetic or electrophoretic properties after 8 months of storage. These findings indicate that red cells stored for a long term in liquid nitrogen can still be used for the study of mutant PK.

Blood Preservation↗
PubMed1979

Peritoneal macrophages introduced into mouse foot pads enter the germinal center of regional lymph nodes nonspecifically.

Male mice were injected into their foot pads with sheep erythrocytes (SRBC) to form lymph follicles in the germinal centers in the popliteal lymph nodes. 4 weeks later, peritoneal macrophages labeled with carbon from syngeneic donors sensitized with SRBC or typhoid-paratyphoid bacilli (TAB) were separately injected into the foot pads as well. The popliteal lymph nodes were histologically examined at 6 h to 5 days after injection. Labeled macrophages appeared in the marginal sinus, migrated straight across the cortex from the marginal sinus to the lymph follicles and then entered the germinal centers. There was no difference in the mode of appearance, migration and localization of labeled macrophages in the regional lymph nodes between the mice given labeled macrophages from SRBC-sensitized donors and those given macrophages from TAB-sensitized donors. The entrance of lymph macrophages into the germinal centers of the regional lymph nodes would be immunologically nonspecific. After the injection of Pelikan ink into the foot pads, the macrophages which have taken up carbon in the peripheral tissue reached the regional lymph nodes via the afferent lymphatics and then entered the germinal centers, mainly through the medullary pole of the lymph follicles, after migrating along their immediate exterior from their marginal sinus to their medullary pole.

Animals↗
PubMed1979

Effects of estrogen on the lymphoid regeneration and immune response in irradiated and marrow-reconstituted mice.

Various doses of estriol (E3) were given to mice intraperitoneally, immediately after lethal irradiation and marrow reconstitution. The assessment of the plaque-forming cell (PFC) response to sheep erythrocytes in the spleen and the histological assessment of lymphoid tissues were carried out 30 days later. The effects appeared to be dose-dependent and resulted in a marked suppression of the PFC response. The depletion of lymphocytes was dramatic and dose-dependent in the thymus, and in the thymus-dependent and in the thymus independent areas of the peripheral lymphoid tissues. These results suggest that E3 acts on the differentiation of stem or precursor cells toweard both the populations of T and B lymphocytes. Although E3, given on day 7 after irradiation and marrow reconstitution, suppressed the lymphoid regeneration and PFC response markedly, E3 given on day 14 had no effect. On day 7 the majority of regenerating lymphoid tissues were large pyroninophilic cells and on day 14, small lymphocytes. These results suggest that the precursor or immature lymphocytes are sensitive to E3, while mature lymphocytes are resistant. Lymphoid regeneration and PFC response were retarded in mice irradiated and reconstituted with bone marrow cells from donors pretreated with E3. These results suggest that E3 acts on the stem or precursor cells capable to differentiate in the direction of lymphoid populations and reduce their number in the bone marrow.

Animals↗
PubMed1979

[An objective technique to examine sensory disturbance in patients with occupational vibration disease. By means of somatosensory evoked response to mechanical stimulation (author's transl)].

Somatosensory evoked responses (SERs) to mechanical (tapping) stimulation were recorded in 24 patients with occupational vibration disease, and they were compared with those recorded in 48 normal subjects. Tapping stimuli were applied on the finger, the palm and the forearm of the bilateral sides. On the other hand, SERs to electrical stimulation were recorded in 5 of the patients, and these were compared with SERs to mechanical stimulation. 1. Some peaks in 32 out of 48 SERs to mechanical stimulation on the finger were absent, and peak latencies in 10 out of the remaining 16 SERs were abnormally prolonged. On the other hand, only 2 out of 27 SERs to mechanical stimulation on the forearm were abnormal. 2. Some peaks in 27 out of 35 SERs recorded in patients with numb sensation in the fingers were absent and peak latencies in the remaining 8 SERs were abnormally prolonged. 3. With respect to the absence of peaks in SERs, P2 and N2 peaks were more frequently absent than N3 and P4 peaks. 4. Peak latencies of the SERs in the patients with occupational vibration disease were abnormally prolonged. The difference of the peak latency between the patients and the controls was statistically significant. 5. Ten SERs to mechanical stimulation and 10 SERs to electrical stimulation were recorded in 5 patients. Nine out of the 10 SERs to mechanical stimulation were abnormal, while only 2 out of the 10 SERs to electrical stimulation were observed. 6. Based on a close relationship between abnormal SERs to mechanical stimulation and the numb sensation in the finger of the patients, it was concluded that sensory disturbances in the occupational vibration disease was due to the lesion in the peripheral nerve. Therefore, SERs to mechanical stimulation can be used for detecting some localized sensory abnormality due to the lesion in the peripheral nerve.

Adult↗