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Biomedical subjects

H Fujii

Publications and source records attributed to H Fujii.

At least 901 records · Page 50Linked to original sources
PubMed1988

Asymmetric transport of D-glucose anomers across the human erythrocyte membrane.

The anomeric preference in the influx and efflux of D-glucose across the human erythrocyte membrane was studied. beta-D-Glucose was transported 1.5 times faster than alpha-D-glucose into the cells, when washed cells were incubated at 20 degrees C in medium containing either alpha- or beta-D-glucose (100 mM). On the other hand, no difference between half-times of efflux of the two anomers was distinguishable. The result demonstrates the presence of influx-efflux asymmetry in anomeric preference in D-glucose transport across the human erythrocyte membrane, and is consistent with the view (Barnett et al., Biochem. J. 145, 417-429, 1975) that the C-1 hydroxyl group of D-glucose interacts with the D-glucose transport protein only in the influx, but not in the efflux.

Erythrocyte Membrane↗
PubMed1988

Brown adipose tissue in Duchenne's progressive muscular dystrophy.

Both granular and multiocular periadrenal brown adipose tissue (BAT) was present in 16 patients with Duchenne's progressive muscular dystrophy (PMD). In 12 cases (75%), diffusely distributed periadrenal BAT was observed. The common cause of death was respiratory failure, and blood gas level showed marked hypoxia. Scattered myocardial fibrosis was seen in all cases of PMD. We suspected that chronic hypoxia and cardiac fibrosis might be largely responsible for BAT transformation in patients with PMD.

Adipose Tissue, Brown↗
PubMed1988

[Intracranial pressure irregularities induced by bulbar stimulation in experimental subarachnoid hemorrhage].

Neurogenic mechanisms of pressure waves were investigated in dogs with acute intracranial hypertension. After subarachnoid infusion of hemolyzed red blood cells, simultaneous recordings of both systemic blood pressure (SBP) and intracranial pressure (ICP) were made continuously. Pressure waves could be induced by electrical stimulation of the medulla oblongata at the stage of increased ICP. Induced pressure waves were specific at the site of the stimulation. These pressure waves were classified into 3 types, consisting of fast waves, slow waves and rebound waves. The fast waves had a duration of 10-30 seconds, being accompanied by a marked increase of SBP. These waves were induced by stimulation of various portions of the lateral reticular formation. The slow waves had a duration of 30 seconds to 3 minutes, being accompanied by a decrease or little change of SBP. These waves were induced by stimulation of the lateral portion of lateral reticular formation. The rebound waves had a duration of 30 seconds to 3 minutes, followed by a decrease of SBP. These waves were induced by stimulation of the lateral area of the solitary tract nucleus. At the end stage of intracranial hypertension, electrical stimulation of all of these sites induced changes of ICP depending on SBP. Slow waves were increase the intracranial pressure during arterial depressor responses indicating primary cerebral vasodilatation. It is suggested from these data that cerebral vascular tonus is concerned with the lateral portion of lateral reticular formation.

Animals↗
PubMed1987

Molecular cloning and sequence analysis of two rat major globin cDNAs.

Two cDNA clones for globins of the adult Wistar rat were isolated from a reticulocyte cDNA library and the nucleotide sequences of the inserts were determined. One clone contained a cDNA insert consisting of 556 bp and the other contained one of 577 bp, both covering the entire coding sequences for rat globins. Comparisons of their predicted amino acid sequences with known sequences of rat globins revealed that these cDNAs coded for a rat major alpha- and a major beta-globin, I alpha and II beta, respectively. The cause of diversity of rat globins was discussed in terms of the nucleotide sequences of cDNAs and known amino acid sequences of globins.

Amino Acid Sequence↗
PubMed1987

Human liver type pyruvate kinase: cDNA cloning and chromosomal assignment.

Pyruvate kinase (PK) has four isozymes (L,R,M1,M2) that are encoded mainly by two different genes. We isolated a cDNA clone from a Japanese adult liver lambda gt10 cDNA library by using a rat liver(L)-type PK cDNA probe. One positively hybridizing clone, hlPK-1, which contained a 1,049-base pair cDNA insert, was subjected to DNA sequence analysis. Comparisons of the sequence data with the rat PK cDNAs indicated that the cDNA encoded information for the carboxyl terminal 105 amino acids of a human L-type PK and a 3' untranslated region of 734 nucleotides. Furthermore, the karyotype analysis of several human-mouse hybrid cells and Southern blot analysis of DNAs of the hybrids with a hlPK-1 indicated that the human L-type PK gene is located on chromosome 1.

Base Sequence↗
PubMed1987

Anomeric specificity of glucose effect on cAMP, fructose 1,6-bisphosphatase, and trehalase in yeast.

The addition of beta-D-glucose (final concentration, 50 mM) to a cell suspension of Saccharomyces cerevisiae in stationary phase caused a rapid 4-fold increase in the concentration of cAMP, while a 2-fold increase of cAMP was observed by the addition of alpha-D-glucose. beta -D-Glucose was also more effective than alpha-D-glucose in the inactivation of fructose 1,6-bisphosphatase and the activation of trehalase. These results, taken together with the previous report that alpha-D-glucose is transported more rapidly than beta-D-glucose in Saccharomyces cerevisiae, do not support the view currently proposed by some investigators that cotransport of D-glucose with protons causes the depolarization of the cell membrane, resulting in the activation of adenylate cyclase. The present data, however, provides supporting evidence for the view that cAMP-dependent protein kinase is implicated in the inactivation of fructose 1,6-bisphosphatase and the activation of trehalase.

Cyclic AMP↗
PubMed1987

Erythrocyte-oxidized glutathione transport in pyrimidine 5'-nucleotidase deficiency.

The oxidized form of glutathione transport was studied in human erythrocytes in pyrimidine 5'-nucleotidase (P5N) deficiency, a disorder in which the amounts of CTP and UTP in the erythrocytes are elevated. The inhibition of ATP-requiring oxidized glutathione (GSSG) transport by CTP and UTP is believed to play a role in elevating the levels of the reduced form of glutathione (GSH) in the erythrocytes of patients with P5N deficiency. The current investigation was undertaken to determine if GSSG transport actually decreases in the erythrocytes of such patients. Erythrocytes from a 17-year-old patient and a 13-year-old patient with P5N deficiency hemolytic anemia and from ten normal subjects were used as materials for the experiment. Erythrocytes, which had been previously incubated with [3H]glycine, were incubated at 37 degrees C, and the rate of [3H]GSSG transported by the cells was estimated. The velocity of GSSG transport out of the erythrocytes was quite low in the patients, 3.17-3.65 nmol GSSG/ml erythrocytes/hr at 37 degrees C in one case, and 3.30 nmol GSSG/ml erythrocytes/hr in the other case, vs that in the normal controls (6.00 +/- 0.80 nmol GSSG/ml erythrocytes/hr; mean +/- SD). The activity of gamma-glutamylcysteine synthetase and glutathione synthetase did not decrease in the patients. Decreased transport activity of GSSG in addition to a normal synthesis rate for GSH may explain the increased concentration of erythrocyte GSH in P5N deficiency.

5'-Nucleotidase↗