Search PubMed⌕ Search

Biomedical subjects

H Fromentin

Publications and source records attributed to H Fromentin.

33 records · Page 2Linked to original sources
PubMedDate not supplied

Ultrastructural study of the interaction of the fungi Sporothrix schenckii and Ceratocystis stenoceras with bone-marrow-derived murine macrophages.

Bone-marrow-derived macrophages of C57BL/6 mice cultivated in vitro were infected with the yeast form of Sporothrix schenckii or Ceratocystis stenoceras. Observations made in light and electron microscopy showed that part of the S. schenckii-containing phagosomes rapidly fused with lysosomes and fungal cells were digested. Surviving fungal cells elongated very rapidly and were liberated into the culture medium after 48 h upon macrophage lysis. The cells of the non-pathogenic isolate C. stenoceras did not elongate and were almost all digested after 8 days, while macrophages were unaltered. Staining for acid phosphatase showed that this enzymatic activity increased soon after ingestion with both isolates. However, this increase was less pronounced with S. schenckii than with the non-pathogenic isolate. The search for a toxic substance produced by S. schenckii and responsible for the low content in acid phosphatase and subsequent macrophage lysis remained negative. It is thus probable that both phenomena essentially resulted from fungus filamentation which led to a dramatic distortion of phagosomes and host cells.

Acid Phosphatase↗
PubMedDate not supplied

[Experimental "per os" transmission of Entomophthora coronata to the mouse].

Mycelial fragments of E. coronata have been observed in the pus of abscesses of the neck and the shoulder of mice inoculated per os, far from the point of inoculation. However, the subcultures were negative; a positive subculture was obtained in only one instance from a lung taken off ten days after the inoculation by the fungus.

Abscess↗
PubMedDate not supplied

[Kinetics of production of collagenase, esterase and elastase by human and soil strains of "Entomophthora coronata" (author's transl)].

A comparative study was made of the production of extracellular proteolytic enzymes, which were originated from two different strains, one pathogenic and one saprophytic, of E. coronata in stable culture at 30 degrees C. Quantitative differences were found: the maximal activity of the three enzymes (collagenase, esterase, elastase) produced by the strain isolated from soil was always lower than that observed with the human strain.

Entomophthora↗
PubMedDate not supplied

[Pathogenicity of "Candida albicans" for normal mice and mice treated by the mycotoxin diacetoxyscirpenol (author's transl)].

The study of the action of diacetoxyscirpenol, a mycotoxin from Fusarium, was carried out on mice. It was found that a single dose of 1/2 DL50 (4.5 mg/kg) inoculated intraperitoneally, induced a transitory diminution of weight of the spleen and thymus and fall in the percentage of blood lymphocyte. The effect on the course of candidiasis was obvious: the mice challenged with C. albicans and treated by small repeated doses of mycotoxin died more rapidly than not treated but infected mice, emphasizing significantly the effect of the immunosuppressive processus on the development of the opportunistic fungus.

Animals↗