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Biomedical subjects

H Friemel

Publications and source records attributed to H Friemel.

At least 37 records · Page 2Linked to original sources
PubMed1978

[Analysis of trends in the immunologic supervision of organ transplantation].

Immunological methods of the rejection diagnostics are discussed. The humoral as well as cullular immune response against the graft is taken in consideration. The tests described up to now in literature are not suited or must still be confirmed, respectively. Thus the aptitude of the nucleolar test, of the leucocyte aggregation test, of the MLC-MEM and the rosette inhibition test for the rejection diagnostics should be increasedly examined. The immunological rejection diagnostics is complicated by the immunosuppressive therapy.

Graft Rejection↗
PubMed1978

[Immunologic course of kidney transplants studied by means of cellular and humoral immunity tests].

In 10 human-allo-transplanted persons and evaluation of the postoperative course was done with the help of specific and unspecific, cellular and humoral immune tests which were compared with clinical parameters. While the E-rosette-test did not show any correlation to the typical courses in the immunoglobulins postoperatively after a clear decrease at the time of the clinically diagnosed first set reaction an increase above all of the IgM could be observed. Unspecific tests, such as CRP and FSP may, concerning the rejection diagnostics, confirm the suspicion in as far as other factors, such as infection of the urinary tract were excluded. By choice of a suitable relation system the evidence of the immunoglobulins could possibly be proved, whereas for the cellular demonstration methods the E-rosette test does not seem to be recommendable in the performed way.

Adolescent↗
PubMed1977

Lymphokines of T and B cells. I. Demonstration that T and B cells of tumor antigensensitised mice produce different lymphokines changing surface charge of target cells.

Lymphocytes from MC sarcoma-bearing mice were exposed to a soluble tumor antigen. The lymphokine-containing supernatant thus obtained was fractionated on Sephadex G-75. Fractions were tested for their activity to reduce the surface charge of indicator macrophages by cell electrophoresis technique. When unseparated spleen or lymph node cells were used the charge-reducing activity of the supernatant was found in three regions according to molecular weights of about 100,000, 60,000 - 35,000 and 13,000 Daltons. The appearance of activity was found to be time-dependent. As indicated by tests with separated lymphocytes both B and T cells appear to release charge-reducing activity.

Animals↗
PubMed1977

[Quantitative immunochemical determination of human secretory IgA].

Secretory IgA from human colostrum was fragmented by mercaptoethanol. The heavy chain fraction was prepared by means of gel chromatography on Sephadex G 100 in 1 N acetic acid. After immunization of rabbits with this heavy chain fraction the resulting antiserum contained antibodies specific for the alpha-chain and antibodies against the secretory component. These antibodies were separated by means of immunosorption. The antibodies against the secretory component precipitate free secretory component as well as bound secretory component. The antiserum is suitable for quantitative determination of secretory IgA, but free secretory component reacts too. Also secretory IgM may react, because secretory IgM contains up to 70% secretory component.

Antibodies↗
PubMed1976

The use of the macrophage electrophoretic mobility test for the diagnosis of eye diseases.

The MEM test by FIELD and CASPARY has been investigated for its importance in analyzing the cell-mediated immunity in ophthalmology. Sensitized lymphocytes of patients are incubated in vitro either with basic encephalitogenic protein for investigating malignoma or with various eye antigens for investigating chronic ocular diseases. The eye antigens have been fractionated by gel chromatography and identified by various immune sera. The sensitized lymphocytes are released in vitro by incubation with antigen a factor which reduces the electrophoretic mobility of guinea pig macrophages.

Animals↗
PubMed1975

[Lymphocyte transformation test in the diagnosis of chronic pancreatitides].

For the immunological diagnostics of the chronic pancreatitis preliminary results on the proof of specifically sensibilized lymphocytes are demonstrated by means of a lymphocyte transformation test. The in-vitro-stimulation is successful in such a case, when no in-vivo-stimulation preceded, i.e. beyond an acute attack.

Antibody Formation↗
PubMed1975

[Immunological studies on the pancreas].

From 1969 to 1974 on 38 diabetic patients with terminal renal insufficiency 1,500 haemodialyses were carried out. Out of them 21 were or are in the prolonged programme of dialysis. The average duration of diabetes up to the terminal renal insufficiency was 20 years. The survival time under dialysis between 50 to 616 days was on the average nearly 248 days. The waste of substances normally contained in the urine and the normalisation of changes of minerals under dialysis is to be compared with that one in non-diabetics. The conduction of the diabetic metabolism in advanced diabetic nephropathy is independent on the form of therapy chosen difficult and undergoes strong variations. For this practical recommendations are given. Dependent on the beginning of the dialysis in 8 cases we succeeded in a temporarily limited full rehabilitation, 5 patients were partially rehabilitated and in 8 patients the general condition could be improved by the treatment without successful rehabilitation. The main complications, which were also dominating causes of death, were seen from the side of the system of coronary circulation. Mediascleroses of the arterial walls partly of a high degree allow the supposition that in these cases additionally a secondary hyperparathyroidism was in question.

Animals↗
PubMed1975

[Immunochemical demonstration of soluble antigens in the protein fractions of placental tissue].

Protein fractions were prepared from placental tissue by homogenization, ammonium sulfate fractionation, gel filtration and ion exchange chromatography. The antigens detected in these fractions by absorbed antisera against placental tissue and pregnancy serum partly occur in the pregnancy and fetal serum too. The other antigens, which are found neither in pregnancy serum nor in fetal serum, could be specific for placental tissue. Their possible significance is discussed.

Antigens↗
PubMed1975

[Cellular immunity to donor antigens after kidney transplants in humans].

Decisive for controlling renal transplantation is the knowledge of the immunological reactions. The cellular immuno-mechanisms, released via sensibilization by donor HL-A antigens, are specially important for acute rejection. To predict a crisis of rejection and to initiate increased immuno-suppressive measures it is important to know the degree of cellular immunologic response. The sensibilization was demonstrated in the experiments by means of the MEM test, using graft antigens isolated from spleen or kidney of the donor. In all patients an increasing sensibilization against donor antigen could be proved. The velocity of this sensibilization seems of value for predicting the duration of the graft's survival.

Electrophoresis↗