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H Fiebig

Publications and source records attributed to H Fiebig.

At least 73 records · Page 4Linked to original sources
PubMed1985

[Characterization of monoclonal antibodies to human monocytes].

A set of monoclonal antibodies reacting with human peripheral blood monocytes is characterized. Two of these antibodies also bind to a fraction of granulocytes (BL-M/G-1) respectively to all of them (BL-M/G-2). On the other hand the BL-M-3 appears to be specific for monocytes only. All three monoclonals do not react with enriched B and T lymphocytes, platelets, erythrocytes and several lymphoid or erythroid permanent cell lines. The antigens recognized by all three monoclonals are expressed by monoblastic cell line U-937, whereas the myeloid line HL-60 and the cell line KG-1 express only antigen recognized by monoclonal antibody BL-M/G-2.

Animals↗
PubMed1985

[Characterization of monoclonal antibodies to human Ia antigens].

A set of monoclonal antibodies reacting with human Ia antigens is characterized. All of these antibodies (BL-Ia/1-3) have the same binding pattern to different types of leukocytes and a set of lymphoid and myeloid cell lines. Monoclonal antibody BL-Ia/1 can be used for complement dependent cytolysis of B cells and Ia+-T cells.

Animals↗
PubMed1985

[The quantitative determination of the IgM rheumatoid factor using a solid-phase radioimmunoassay. Comparison with the agglutination test and the radioimmuno-polyethyleneglycol precipitation test].

A solid-phase radioimmunoassay capable of detecting nanogram quantities of human IgM rheumatoid factor using a monoclonal anti-mu-chain antibody is described. Human IgG did not interfere with the detection of IgM RF by this method. The small nonspecific binding of nonRF IgM to the human IgG coated tubes utilized in the assay must be corrected for by assaying samples in parallel bovine serum albumin coated control tubes only in cases of deviation of IgM from normal range. 69 coded and randomly arranged sera from patients with rheumatoid arthritis (RA), nonrheumatic joint diseases and healthy adult control subjects were investigated by this method, agglutination techniques as well as RIPEGA. A good correlation between solid-phase radioimmunoassay and agglutination techniques was found. Patients with seropositive RA had significantly higher concentrations of IgM RF than seronegative RA patients or control subjects (mean +/- 1 SD = 133,3 +/- 187,2 micrograms/ml versus 4,7 +/- 6,5 micrograms/ml and 2,2 +/- 4,0 micrograms/ml; resp.).

Agglutination Tests↗
PubMed1984

Conformational changes induced by hapten in murine monoclonal antibodies to dinitrophenyl groups--the analysis by temperature-perturbation spectroscopy.

Two samples of murine monoclonal antibodies to dinitrophenyl groups were studied by difference thermal perturbation spectroscopy with particular attention to changes in the amount of perturbed chromophores induced in antibodies as a result of hapten binding (epsilon-2,4-dinitrophenyl-L-lysine). Despite the fact that both antibody samples belong to immunoglobulin G1 and have the same type of light chain, kappa, they were found to differ significantly in the number of the chromophores perturbed by temperature. The binding of hapten decreases the perturbation of chromophores only in the sample with the less rigid structure, as regards thermal perturbation. These data provide evidence that differences in the rigidity of the structure of variable domains affect the extent of conformational changes induced in the antibodies due to the interaction with an antigen.

Animals↗
PubMed1984

[Characterization of a series of monoclonal antibodies against human T cells].

A set of 6 monoclonal antibodies is characterized reacting only with human T-cells but not with B lymphocytes. The antibody BL-T1 binds to the E receptor, being there on all mature T cells and on the majority of thymocytes. All respectively the majority of peripheral T cells but only subpopulations of thymocytes are recognized by the monoclonals BL-T2, BL-T3, BL-T4 and BL-T5. For identification and quantification of mature human T cells especially the antibody BL-T2 is suitable. The antigen recognized by BL-E1 is missing on peripheral B lymphocytes but expressed not only on T cells but also on erythrocytes, monocytes and a fraction of granulocytes. The monoclonals discussed are compared with the monoclonals for identification of leucocyte differentiation antigens (cluster of differentiation) characterized on the 1st Int. Workshop on Human Leucocyte Differentiation Antigens (Paris, 1982).

Animals↗
PubMed1984

[Immunodiagnosis of chronic lymphatic leukemia and leukemic non-Hodgkin's lymphoma with the monoclonal antibodies BL-Ig-L/1, BL-DR/1 and BL-T2].

3 self made monoclonal antibodies are tested with the indirect immunofluorescence method for the immunologic diagnostic of the circulating lymphocytes from 24 chronic lymphatic leukaemia (CLL) and 14 leukaemic Non-Hodgkin lymphoma (NHL) patients. In comparison, investigations were done with various specific rabbit antibody F(ab)2 fragments and antisera. The monoclonal antibody BL-Ig-L/1, which is directed to the human Ig L-chains, marked the neoplastic lymphocytes from 12 of 24 CLL and from 9 of 14 leukaemic NHL as membrane Ig+. The monoclonal antibody BL-DR/1, which reacts with a HLA-DR, binds to the blood lymphocytes of 23 CLL and 11 NHL patients. By this BL-DR/1 is superior to BL-Ig-L/1 for the immunologic diagnostic of the non-T-cell neoplasia. The with normal peripheral T-cells reactive monoclonal antibody BL-T2 reacts with malignant B lymphocytes on an equal scale as BL-DR/1. It is not qualified for the differentiation of malignant blood lymphocytes.

Antibodies, Monoclonal↗
PubMed1983

[Slide test for immunofluorescent microscopy and immunoenzyme detection of cell-membrane antigens].

Poly-dimethyl-diallyl-ammoniumchloride - coated glass slides cause a strong adherence of living cells in consequence of electrostatic interactions. Immunological reactions of adherent cells - we used immunofluorescence and immunoperoxidase techniques to demonstrate membrane antigens - are not impaired. The slide test is suitable for routine quantitation of B and T cells in the peripheral blood and for the selection of hybridoma antibodies against cellular antigens. The slide test is time saving and needs minimal volumes of cells and antisera.

Antigens, Surface↗
PubMed1983

[Immunohistological detection of T lymphocytes with monoclonal antibody BL-T2].

Using the monoclonal Pan-T cell antibody BL-T2 T lymphocytes are identified on tissue sections with an indirect immunofluorescence technique. The T cell areas between the lymphoid follicles (B cells) are stained on cryostat sections of human palatine tonsils. A conventional heterologous anti-Ig antiserum was used for the detection of B lymphocytes. The identification of T and B lymphocytes by immunofluorescence allows statements about the distribution of both populations in tissue sections.

Antibodies, Monoclonal↗
PubMed1982

Glomerular immune complex deposition, circulating immune complexes, and antibodies in experimental insulinopenic diabetes.

Compared with control animals with a normal metabolism, rats with insulinopenic diabetes generally show an increase in glomerular deposition of complement-fixing immune complexes after immunization with bovine albumin and bovine gamma-globulin. Compared with the control group, the serum of the diabetic animals showed a reduction in the titers of IgM-isotype antibodies, which have a lower affinity. The concentration of the circulating immune complexes is the same. The increased frequency of glomular deposits in experimental diabetes can be explained by an increase in capillary permeability and by the formation of qualitatively different immune complexes.

Animals↗
PubMed1980

[Evolutionary origin of the T-lymphocyte receptor. II. Production and partial characterization of antisera to the immunoglobulin-like cell membrane protein from thymocytes of the carp].

An immunoglobulin-like protein from Triton X-100 solubilized cell surface proteins from thymocytes can be isolated by specific precipitation using anti-IgM antibodies. Immunizing rabbits with those immune complexes we succeeded to elicit anti-thymocyte mIg sera reacting with both thymocyte mIg and IgM. SDS-PAGE analysis revealed that anti-IgM and anti-thymocyte mIg sera recognize the same thymocytic surface protein. The cross-reactivity between thymocyte mIg and IgM is caused by protein determinants very similar to each other. Anti-thymocyte mIg sera absorbed with IgM lose their capacity to bind thymocyte mIg. However, following absorption with thymocytes anti-IgM sera still possess amounts of antibodies reacting with humoral IgM.

Animals↗
PubMed1980

[Structure of antibodies and the specificity of the antigen-antibody interaction (author's transl)].

A review is given about some aspects of the antigen-antibody interaction. Especially the specificity of antibodies to haptens and the binding forces are viewed. Some problems of the nature of antigenic determinants of globular proteins are discussed. Models of structure of antigenic determinants on such proteins are presented. The structural basis of antibody molecules, especially of the combining site are looked in review. Theories are presented, which are suitable to explain the high specificity and the great heterogeneity of antibodies. The concept of multispecificity of a single antibody molecule in relation to the high specificity of antisera is discussed.

Animals↗