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Biomedical subjects

H Festenstein

Publications and source records attributed to H Festenstein.

At least 181 records · Page 10Linked to original sources
PubMed1977

Studies on H-2 specificities on mouse tumour cells by a new microradioassay.

Seven mouse tumour cell lines of different aetiology and origin were tested for the expression of surface alloantigens using twenty-four well defined H-2 alloantisera and anti-Thy 1.2. We used a new radioassay that involves antibody-complement treatment of the tumour target cells followed by postlabeling the surviving tumour cells with 14C-thymidine. With a relatively high frequency the anti-H-2 sera were reacting differently with the tumour cells than with respective syngeneic lymphoid cells. Thirty-six anomalous reactions out of 129 investigated were detected. Absorbtion experiments performed with H-2 antigen positive or negative lymphoid cells revealed a striking similarity between these extra-specificities and H-2 specificities of foreign haplotypes. The results are discussed in relation to the biological importance of the extra-specificities, both with regard to origin (derepression) and function (transplantation antigen properties).

Animals↗
PubMed1977

Cytostatic effector cells generated in vivo against M locus determinants.

Cytostatic effector cells against M-locus (Mls) determinants were generated in mice by immunization with Mls incompatible lymphocytes. A post-labelling assay of 14C-labelled uridine uptake by macrophage targets was employed to meaure the inhibitory activity of the effector cells. Using lymphocytes from Mls pseudocongenic mice to immunize donors of effector cells, it was confirmed that the cytostatic effect was via the Mls determinants expressed on the macrophage targets. Cytotoxic effector cells against M-locus (Mls) determinants were not detected by the cell-mediated lympholysis assay (CML) in a five day mixed lymphocyte culture (MLC) between Mls incompatible lymphocytes. Specificity of cytotoxic cells generated by culturing in vivo preimmunized responder cells and Mls incompatible stimulator lymphocytes did not correlate with the Mls determinant of the stimulator lymphocytes.

Animals↗
PubMed1977

HLA-D-locus determinants detected by sperm-lymphocyte culture.

Twenty-seven sperm from 17 donors were tested against a random panel of sperm donors' lymphocytes and others and used to identify HTC-defined HLA-D determinants as well new SLC-defined determinants including DW Fes 5; the latter was confirmed as a new D-locus specificity by family studies.

Chromosome Mapping↗
PubMed1976

HLA genotyping by using spermatozoa: evidence for haploid gene expression.

Serologically defined HLA-A and HLA-B antigens are expressed in a haploid manner on the surface of spermatozoa. This enabled the HLA-genotypes of four men to be determined by using sperm. This technique will mean that the HLA genotype of potential male graft recipients and others can be determined more easily and even when families are not available. These findings also provide strong evidence that some cell products are encoded by a haploid genome.

Adolescent↗
PubMed1976

Modification of murine T-cell cytotoxicity by preimmunization with M locus and H-2 incompatibilities.

The effect of preimmunization of CBA/H mice with M locus and H-2 incompatible lymphocytes was examined on the in vitro generation of cytotoxic cells to both types of targets. Preimmunization with M locus did not help to generate cytotoxic cells to M-locus targets; however, the anti-H-2 cytotoxicity of M-locus-preimmunized lymphocytes was suppressed between the 6th and 19th day after preimmunization. In contrast, preimmunization with H-2 incompatibility produced an amplified lytic anti-H-2 response. The possible mechanisms are discussed.

Animals↗
PubMed1976

Influence of HLA matching and blood-transfusion on outcome of 502 London Transplant Group renal-graft recipients.

The outcome of 502 cadaver kidney transplants has been followed for up to six years; these grafts were arranged through the Tissue Immunology Unit of the London Hospital Medical College, the coordinating centre of the London Transplant Group. An analysis of HLA (A and B) recepient-donor matching revealed, as in previous analyses, clear differences (now highly significant) between the best as compared with the lesser matched recipients. A quarter of the patients (group 4 and 3a) had a superior outcome 20-30% greater than poorly matched (2 or less group) which constituted 53% of individuals. The results in the 3b group (28% of patients) were intermediate 10-15% better than the "2 or less antigens in common" group. A small number of recipients mostly 4 or 3 matched who were retrospectively HLA-D matched showed an even better graft survival. The effect of blood-transfusion before transplantation was studied and found to improve the outlook especially in the best-matched groups. No difference was apparent between those receiving less or more than ten units except in a group of patients with cytotoxic antibodies and/or retransplants. This "immunocompetent-presensitised" group had the best outcome provided these recipients had few transfusions and were subsequently well matched. These findings emphasise the continued need for successful collaborative associations, so that improved matching can be achieved which if universally applied would ensure better graft survival for a large number of patients in renal failure.

Binding Sites, Antibody↗
PubMed1976

Serological identification of Ia antigens: report of a British region Ia workshop.

In preparation for the 7th International Histocompatibility Workshop 13 laboratories in the British Region participated in a local workshop. One hundred and twenty-three sera which had been previously shown to have activity on either normal B cells, CLL cells or B cell lymphoid lines in the absence of HLA-A, B or C activity were exchanged between the laboratories. These sera were tested on a total of 212 B cells, 101 CLL cells, 76 T cells and 76 lymphoid cell lines. The data was collected and analyzed in Oxford. The analysis showed that six groups of sera could be distinguished. When these groups were compared with the D locus typing of some of the lymphoid lines which were derived from individuals used as MLC typing cells, they were seen to have significant associations with D locus antigens. The serological groups defined were therefore given numbers corresponding to the D locus numbers they associate with, i.e. UK1 is associated with DW1 and so on for UK2, 3, 4, 5 and 7. Comparison of typing techniques showed that long incubation both with antiserum and then with complement, 1 hour + 2 hours gave the best and most reproducible reactions on normal B cells. Residual anti-HLA-A, B or C activity in some of the sera even after platelet absorption showed the importance of adequate checking on T cells after absorption.

Antibody Specificity↗