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Biomedical subjects

H Feng

Publications and source records attributed to H Feng.

At least 73 records · Page 4Linked to original sources

Three-dimensional finite element analysis and comparison of stress distribution in overdentures supported with bar attachments and telescopic crowns.

OBJECTIVE: To determine which one of the overdenture (OD) retainers was more beneficial to the abutments--the bar or the telescopic crown--and if a tooth could be used as an OD abutment when the supporting bone decreased to half of the root. MATERIALS AND METHODS: The three-dimensional finite element method was used to investigate the stress distribution in a mandible with an overdenture supported by the roots of two canines. The two teeth were either connected with a bar attachment or restored with telescopic crowns, and were then restored with a complete overdenture. For each type of superstructure, the influence of bone height around the roots (no alveolar resorption and resorption to half of the root length) was analyzed under three loading conditions. RESULTS: The most extreme stress values were located at the alveolar ridge crest of the bone around the root of the abutment. In the case of anterior loading, the largest compressive stress (LCS) in telescopic crown overdenture (TOD) was lower. When loaded posteriorly there was no obvious difference between bar attachment overdenture (BOD) and TOD. At the alveolar ridge crest around the root of the abutment, the stress value in the model with decreased bone level was higher than in the model with normal bone level, but the range was not significant. CONCLUSION: 1. In terms of stress in the alveolar bone, complete overdenture over telescopic crowns is more convenient than over bar attachments. 2. Stress increases as the height of the bone around the roots decreases, but the increment is less than 30%. It is important for an abutment with bone resorption to perform root amputation to obtain an acceptable crown/root ratio.

Alveolar Bone Loss↗

[RAB5A, a gene possibly related to metastasis of human carcinoma of the lung and stomach].

OBJECTIVE: To study and isolate tumor metastasis-related genes, and to explore the molecular basis of metastasis of lung and gastric carcinoma. METHODS: Using mRNA differential display technique, cDNA cloning and sequencing, RT-PCR, Northern blot and immunocytochemical methods, genes differentially expressed between human lung adenocarcinoma cell lines AGZY83-a and Anip973 were detected. Anip973 was isolated from AGZY83-a with much higher metastatic potential than the parent line. RESULTS: There were significant differences in gene expression between the two cell lines. RAB5A was one of the genes differentially expressed in Anip973 cells. Its gene product is a GTP-binding protein. Paraffin sections of 33 lung carcinoma and 47 gastric carcinoma specimens were examined immunohistochemically. Expression of RAB5A was positive in 87.9% of NSCLC and 91.5% of gastric cancer specimens examined (P < 0.05), and correlated with their metastatic potential and differentiation degree of gastric carcinoma. CONCLUSION: RAB5A is possibly a metastasis-related gene.

Humans↗

[Plasma level of glucagon in cirrhotic patients with portal hypertension during operation of portacaval shunts].

OBJECTIVE: To investigate the plasma level of glucagon in cirrhotic patients with portal hypertension during operation of portacaval shunts. METHODS: Plasma level of glucagon in the portal venous, peripheral venous and arterial blood in cirrhotic patients (n = 16) and control patients (n = 16) was measured by radioimmunoassay respectively. RESULTS: The glucagon ratio (pg/ml) of the hepatic cirrhosis group before, during after operation 3 days after operation, 7 days after operation was observed portal venous blood: (189.2 +/- 58.7) pg/ml, (196.1 +/- 54.2) pg/ml vs. (101.9 +/- 44.8) pg/ml, (98.2 +/- 34.1) pg/ml (P < 0.01); peripheral venous blood (184.1 +/- 48.2) pg/ml, (187.5 +/- 42.8) pg/ml vs. (102.4 +/- 34.7) pg/ml, (95.2 +/- 24.9) pg/ml (P < 0.01); arterial blood: (186.3 +/- 51.3) pg/ml, (183.5 +/- 54.2) pg/ml vs. (103.4 +/- 36.3) pg/ml, (93.9 +/- 30.6) pg/ml (P < 0.01). The portal vein pressure was positively correlated to the plasma level of glucagon in portal venous blood of cirrhosis (r = 0.57, P < 0.01). CONCLUSIONS: Hyperglucagonemia may be caused by the decreased glucagon metabolism in cirrhotic liver and hypersecretion of pancreas and the gastrointestinal tract. Hyperglucagonemia may be amended after portacaval shunts.

Adult↗

[The levels of renin activity, angiotensin converting enzyme and angiotensin II in cirrhotic patients with ascites undergoing portacaval shunt].

OBJECTIVE: To investigate the changes of plasma renin activity (PRA), angiotensin converting enzyme (ACE), angiotensin II and portal venous pressure (PVP) in cirrhotic patients with ascites undergoing portacaval shunt. METHODS: Serum PRA, ACE, A II levels in portal vein, artery, peripheral vein and PVP were measured in 16 cirrhotic patients with ascites before and after portacaval shunts and in control group of 16 cases of gastrointestinal carcinoma. By light colorimetric analysis and radio-immunization assay. RESULTS: PRA, ACE, A II levels and PVP of pre- and after portacaval shunt were significantly higher in cirrhotic patients than the control group (P < 0.05), and they were significantly decreased after the shunt in the cirrhotic patients (P < 0.05). The PVP was in positive correlation to the levels of serum ACE in cirrhotic patients (r = 0.48, P < 0.01). CONCLUSIONS: The levels of serum PRA, ACE, A II and PVP decreased significantly in cirrhotic patients with portal hypertension after portacaval shunts. It may be major causes of ascites disappearance in cirrhotic patients after portacaval shunt.

Adult↗

[Synergistic interaction between Ys-96, a bisbenzylisoquinoline compound derived from Stephania tetrandra, and adriamycin or vincristine against human cancer cell lines in vitro].

OBJECTIVE: To study in vitro the anticancer interaction between Ys-96, a bisbenzylisoquinoline compound derived from Stephania tetrandra, and adriamycin or vincristine against human cancer cell lines. METHOD: Using human breast cancer cell MCF-7 and its adriamycin-resistant cell line MCF-7/Adr, and human nasopharyngeal cancer cell KB and its vincristine-resistant cell line KBv200 in an in vitro system, anticancer interaction between Ys-96 and adriamycin or vincristine was evaluated with a method reported by Berenbaum. RESULT: The SFIC values (sum of fractional inhibitory concentration) of the combinations with 3 different ratios between Ys-96 and adriamycin or vincristine were markedly less than 1.0, and the shapes of all the isobologram curves were concave. CONCLUSION: The synergistic interaction between Ys-96 and adriamycin or vincristine against the above human cancer cell lines was positively observed in vitro.

Antineoplastic Agents, Phytogenic↗

[The design of a multi-parameter patient monitor].

This paper describes the software and hardware design of a multi-parameter patient monitor which is able to measure ECG, heart rate, blood pressure, breath rate, body temperature and oxygen saturation non-invasively. The instrument uses embedded industry control module as its main controller and 9" CRT as its display. Several OEM modules are utilized in the design, such as ECG module, blood pressure module and oximeter module. This design strategy has the features of reliable performance, easy maintenance, and short developing period.

Diagnostic Equipment↗

Over-expression of the RAB5 gene in human lung adenocarcinoma cells with high metastatic potential.

The objective of this study is to better understand the molecular mechanism of tumor invasion and metastasis, and isolating tumor metastasis-related genes. Two human lung adenocarcinoma cell lines AGZY-83a and Anip973 were studied. Anip973 was derived from AGZY-83a, but it manifested a very much higher metastatic potential than the parent line. Differential cDNA fragments were isolated by using the techniques of mRNA differential display, and analyzed by means of molecular cloning and sequencing. The expression of RAB5A gene in clinical samples of non-small cell lung cancer was determined by RT-PCR. There were significant differences between AGZY-83a and Anip973 in gene expression. Part of the differential cDNA fragments were cloned and sequenced. We found that there was over-expression of RAB5A gene in the Anip973 cell line. And there was over-expression of RAB5A gene in those samples of clinical lung cancer showing metastasis. In conclusion, the expression or over-expression of RAB5A gene was associated with the metastatic phenotype of Anip973. Probably, over-expression of RAB5A gene in non-small lung cancer may serve as a diagnostic marker for metastasis.

Adenocarcinoma↗

[Spectroscopic assay, chemical modification and activity of three kinds of polyoses].

Infrared spectra and nuclear magnetic resonance spectra of chitosan, pachyman and amorphalls mannan were studied. The difference among the infrared spectra of chitosan under different conditions was disscused. The modification of Methylolation of the three kinds of polyoses was made and the relationship between the chemical modification and the antitumor activity was also studied.

Antineoplastic Agents↗

[Study on induced mutagenesis interaction of the high temperature and/or cigarette smoke].

The study aim is to research the genotoxicity and interaction of the High temperature and/or cigarette smoke. The micronucleus of human lymphocytes and bone marrow in mice were observed following heat stress and/or watersoluble cigarette smoke substances. The results indicated that the high temperature (40 degrees C, 41 degrees C and 42 degrees C for 5 min, respectively) significantly induced micronuclei in human lymphocytes in vitro (P < 0.01). A significant synergistic effect of the high temperature and cigarette was found. When high temperature combining with high concentration cigarette smoke (1.0-2.0 cigarette/20 g/d, for 10 d) was used, a significant induction and interaction (P < 0.01) on micronucleus of the bone marrow were also observed. The results indicated that high temperature substanance aberrations would be enhanced when the high temperature combines with cigarette smoke.

Animals↗

[Effects of taurine and extraction of cristata L on serum Zn, Cu and Ca in rats].

Forty rats fed with basic diets were randomly divided into 4 groups. NG group were fed with basic diets. The other three groups were fed with high fat diets. The rats in TA group and EC group were given TA 100 mg/kg or EC 100 mg/kg each day respectively in addition to high fat diet for 8 weeks. The results showed that taurine and extraction of cristata L not only increased the level of red cell SOD and the content of serum Zn (P < 0.05), but also decreased the contents of TC, MDA in the wall of artery and decreased the level of serum LDH significantly (P < 0.05 or P < 0.01). TA and EC increased significantly the content of serum Cu and decreased the ratio of serum Cu to Zn in the high fat diet rats (P < 0.01), and decreased the contents of serum Ca also. The results indicate that TA and EC may play some role in lipid metabolism and inhibit atherosclerosis by regulating the levels of Zn, Cu and Ca in rats.

Animals↗

The c-kit receptor and its possible signaling transduction pathway in mouse spermatozoa.

The presence and role of the c-kit protein was investigated in the mature sperm of the mouse. The c-kit monoclonal antibody (mAb) ACK2 reacted specifically with the acrosomal region and the principal piece of fixed noncapacitated sperm but did not react with the acrosome region in acrosome-reacted sperm. ACK2 significantly inhibited the acrosome reaction; this inhibition was relieved by the calcium ionophore A23187. The kit ligand stem cell factor (SCF) significantly increased the percentage of sperm undergoing acrosome reaction. This increase was partially inhibited by the calcium channel inhibitor (verapamil), the PI3k inhibitor (wortmannin), and the PLC inhibitor (U-73122). ACK2 predominantly recognized c-kit proteins of 33, 48, and 150 kDa by Western blotting of mouse sperm extracts. The 48- and 150-kDa protein bands were released into the media and tyrosine autophosphorylated at low basal levels during acrosome reaction. On stimulation with SCF, the level of c-kit phosphorylation increased significantly. These findings suggest that c-kit is present in mature sperm, and its binding to SCF may result in the activation of PLC gamma 1 and PI3K, leading to receptor autophosphorylation, and ultimately may play a role in capacitation and/or the acrosome reaction.

Animals↗

2D CP/MAS 13C isotropic chemical shift correlation established by 1H spin diffusion.

A new 2D solid-state CP/MAS 13C NMR exchange experiment for through-space isotropic chemical shift correlation is proposed and demonstrated. Through-space correlation is established via a second cross polarization from 13C to 1H and subsequent 1H spin diffusion. A third cross polarization results in the final 13C-13C isotropic chemical shift correlation. The 1H spin diffusion time is a variable parameter allowing different mean square magnetization displacements to be probed. Experimental results on mixtures of differently 13C-labeled alanine and polyethylene indicate that this site-selective 2D technique can be used to characterize domain sizes and proximities over a wide range of length scales (1-200 nm) in solids such as polymers or biological materials.

Alanine↗

[Reversal effect of Ys-96, a bisbenzylisoquinoline, on adriamycin or vincristine resistance in human cancer cells in vitro].

OBJECTIVE: Reversal effect of Ys-96, a bisbenzylisoquinoline, on the resistance of human cancer cells to adriamycin or vincristine was studied in vitro. METHOD: In an in vitro culture system of human cancer cells MCF-7 and its adriamycin-resistant line MCF-7/Ad or KB and its vincristine-resistant line KBv200, the sensitivity(IC50) of the resistant cell lines to adriamycin or vincristine was evaluated with a MTT assay. RESULT: IC50 value of adriamycin or vincristine in combination with Ys-96 at a concentration of 1.00 mumol/L against MCF-7/Ad or KBv200 was found to be close to that of adriamycin alone or vincristine alone against the sensitive cell line MCF-7 or KB. CONCLUSION: The drug resistance of MCF-7/Ad or KBv200 could be essentially reversed by Ys-96 at a concentration of 1.00 mumol/L.

Benzylisoquinolines↗

[Histamine-1 and -2 receptors in hepatic tissues of cirrhotic patients with portal hypertension].

OBJECTIVE: To study histamine-1 and histamine-2 receptors in hepatic tissues of cirrhotic patients with portal hypertension. METHOD: Light autoradiography was used. RESULT: The density of histamine-2 (H-2) receptors (cirrhotic patients/controls) in hepatocytes was 168.4 +/- 22.6/511.9 +/- 37.6 (P < 0.01), in hepatic vein 238.8 +/- 40.7/313.0 +/- 52.4 (P < 0.01), in hepatic artery 55.6 +/- 7.1/175.4 +/- 26.1 (P < 0.01), and in portal vein 52.0 +/- 5.4/166.4 +/- 17.7 (P < 0.01). The density of histamine-1 receptors in hepatocytes, hepatic vein, hepatic artery and portal vein was not statistically changed between cirrhotic patients and controls (P > 0.05). CONCLUSION: The density of H-2 receptors in hepatic tissues of cirrhotic patients decreased significantly.

Adult↗

[Effect of somatostatin on portal and hepatic hemodynamics and portal venous pressure in cirrhotic patients with portal hypertension].

OBJECTIVE: To investigate the effect of somatostatin on hepatic and portal hemodynamics and portal venous pressure in cirrhotic patients with portal hypertension. METHOD: Colorful doppler ultrasonography was used to measure the diameter and maximum hemokinetic velocity of portal vein and hepatic vein before and after intravenous administration somatostatin, and thus their hemokinetic volume was calculated respectively. Among 15 surgical patients, changes of the portal venous pressure were observed via a catheter before and after the administration of somatostatin. RESULT: One hour and 1.5 hour after the administration, the pressure of portal vein significantly decreased from 2.77 +/- 0.26 kPa to 2.42 +/- 0.27 kPa and 2.40 +/- 0.27 kPa, respectively (P < 0.01). The systolic pressure, diastolic pressure and heart rate showed no significant changes. After the administration, the sectional area of portal vein decreased by 7.28% but without significance. The maximum hemokinetic velocity of portal vein significantly decreased by 18.96% (from 19.72 +/- 7.75 cm/sec to 15.98 +/- 7.26 cm/sec, P < 0.01). The mean total hemokinetic volume increased from 1786.22 +/- 926.37 ml/min to 1836.17 +/- 844.24 ml/min, but without significance (P > 0.05). CONCLUSION: Continuous instillation of somatostatin via peripheral vein can decrease the portal venous pressure in cirrhotic patients with portal hypertension. The mechanism may be the decrease of hemokinetic volume caused by the decrease of hemokinetic velocity of the portal vein.

Adult↗

[The structures elucidation of isodopharicin D and F].

Two new compounds were isolated from Isodon pharicus (Prain) Murata. Their structures were determined to be 3 alpha, 11 beta, 13 alpha-trihydroxy-entkaur-16-en-15-one (1), named isodopharicin D, and 11 beta, 13 alpha, 15 alpha-trihydroxy-entkaur-16-en-3 alpha-beta-D-glucoside (2), named isodopharicin F by chemical and spectral evidence.

Diterpenes↗

[Establishment and discretization of human torso computer images for ECG simulation].

The establishment and discretization of human torso computer image is an important aspect in studying of ECG simulation. A rapid and effective method is presented in this paper, which establishes and discretes image of human torso exactly by curve fitting and by introducing concept of "nihility point". It also has some value for solving problem of discreting field region in numerical calculation of electromagnetic field.

Algorithms↗