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Biomedical subjects

H F Wang

Publications and source records attributed to H F Wang.

43 records · Page 3Linked to original sources
PubMed1986

[Infiltrating lymphocytes in esophageal carcinoma--II. Subsets of T lymphocytes].

The distribution of T lymphocytes and it's subsets in cancerous tissues, local lymph nodes and peripheral blood of patients with esophageal carcinoma were investigated. The percentages of total and active T cells in the peripheral blood and lymph nodes were within normal range. Most of the lymphocytes infiltrating in the cancerous tissues are T cells. It suggested that cellular immunity be developed in the cancerous tissues. The percentage of T mu in the patient's blood was lower than that of the normal person but the T gamma was higher. Proportions of T mu and T gamma in cancerous tissues were paralleled with those in blood, i.e. T gamma is higher than T mu. The mean percentage of T mu in lymph nodes was 25.52 +/- 14.06% and T gamma was 3.28 +/- 2.33%. It seems likely that the former is lower than normal and the latter is elevated.

Adult↗
PubMed1977

Variation of deoxyribonucleic acid polymerase activities during avian erythropoiesis.

Amounts of DNA polymerase alpha and beta were determined in extracts of chicken erythroid cells at various stages of development. Concentrations of both polymerase activities are high in erythroblasts which are still dividing, decline after the cells cease dividing and begin maturation, and become almost undetectable in the fully mature erythrocytes. While DNA polymerase alpha activity declines gradually, firmly bound DNA polymerase beta activity in the nuclei drops abruptly after the cells finish DNA synthesis and dividing. The amount of a low molecular weight DNA polymerase extractable with the cytoplasmic fraction, possibly DNA polymerase beta, is low in erythroblasts, increases in the more mature erythroid population and then declines to an undectable level in the fully mature erythrocytes.

Anemia, Hemolytic↗
PubMed1976

Evidence that both growing DNA chains at a replication fork are synthesized discontinuously.

Escherichia coli, Bacillus subtilis, and T7-infected E. coli have been labeled with short pulses of [3H] thymidine, and the labeled DNA has been examined by sedimentation in alkaline sucrose. In all three systems, the great majority of the DNA labeled by a short pulse is found in the form of small DNA chains of 10S, the so-called Okazaki pieces. The B. subtilis and T7 nascent DNA fragments hybridize with equal efficiency to the separated strands of B. subtilis and T7 DNA, respectively. The results suggest that both growing DNA chains at a given replication fork are synthesized discontinuously in the case of E. coli, B. subtilis, and T7. We have found that the method used to terminate the pulse affects the size distribution of the labeled DNA; some methods allow joining of nascent DNA fragments after termination of the pulse. Previous reports of discontinuous DNA synthesis on only one growing DNA chain and continuous synthesis on the other DNA chain are probably due to preferential joining of Okazaki pieces on the DNA chain growing in the overall 5' leads to 3' direction.

Bacillus subtilis↗