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Biomedical subjects

H F Lee

Publications and source records attributed to H F Lee.

34 records · Page 2Linked to original sources
PubMed1991

[Improvement of the EIA kit for the detection of tetanus immunoglobulin in human sera].

For the program to eliminate tetanus neonatorum in this country, we have improved the sensitivity of enzyme immunoassay kit (EIA kit) prepared on 1987 for the detection of human tetanus immunoglobulin (TIG) by competitive principle. (Chinese J Microbiol Immunol 1987; 20: 269-278) Horse-radish peroxidase-conjugated to tetanus toxoid monoclonal antibody was involved in the new kit, and tetanus hybridoma clones were prepared by this laboratory. The lowest detectable TIG level is 0.05 IU/ml serum instead of 0.1 IU/ml serum. The dose-response curve and cut-off determination system of the new EIA kit are better than those of the original one. We proposed that the newly designed EIA kit could be used for understanding the TIG level in women who are in the age group for giving birth and in the tetanus vaccination group.

Animals↗
PubMed1987

Serological survey of immune response to tetanus toxoid using antitoxin enzyme immunoassay.

A method for testing serum tetanus immunoglobulin (TIG) was set up using enzyme immunoassay kit (EIA kit) and prepared by competitive principle in this laboratory. Forty-eight human sera tested by both EIA and toxin neutralization test (NT) in mice were in good agreement. Determination coefficient (r2) of the EIA and NT was 0.922. The lowest detectable dose was 0.1 IU/mL ELISA value. Many authors believe this ELISA value to be a safe protection TIG level. For serological survey of immune response to tetanus toxoid, sera from two groups of subjects were examined. Among 83 subjects, each over 40 years of age, only 47.0% were positive to TIG EIA level. Forty-six subjects with undetectable EIA titres to tetanus toxoid (TT) were immunized against two doses of TT at an interval of four weeks; 44 (95.6%) demonstrated TIG concentration of greater than or equal to 0.2 IU/mL within two weeks to one month following the last dose vaccination. The TIG positive rates of 725 school students' sera, selected by multistage sampling from 2395 specimens, were reported. Those were collected by simple random sampling from geographical area in the North, South, Central, Central Mountain, and East areas of Taiwan according to age groups. They were 80.8%, 65.1%, 56.0%, and 42.0% to the age groups of 0-4, 5-9, 10-14, and 15-17 years respectively. The probabilities of each near by two groups were p less than 0.1; p less than 0.05; p less than 0.05. The differences of positive rates in the last three groups were significant.

Adolescent↗
PubMed1985

[Clinical evaluation of a domestically prepared enzyme immunoassay kit for the detection of hepatitis B surface antigen].

National Institute of Preventive Medicine (NIPM) has succeeded in the development of an enzyme immunoassay (EIA) kit for detection of hepatitis B surface antigen. The sandwich principle was used for the test. Guinea pig anti-HBs IgG was used for coating microtiter plates and Horseradish peroxidase was conjugated with goat specific anti-HBs. Its stability is longer than 4 months. The lowest detectable dose is 0.7 ng/ml or better for subtype ad of HBsAg tested with Hepatitis Sensitivity Panel, Bureau of Drug and Food, Department of Health. The regression curve was determined by testing 66 samples with Auszyme II (EIA Kit. Abbott Lab.) and NIPM Kit, while Auszyme II used as a reference kit. The two EIA kits correlated well with a coefficient of determination (r2) of 0.86. Evaluation on 1,157 patients' and officers' serum samples in Tri-Service General Hospital showed that the positive rate was 24.7% (286/1,157) by RIA (Clinical Assays, Travenol Lab., USA) and that of NIPM EIA Kit was 24.4% (282/1,157). There was no statistical significance in terms of positive rate (p greater than 0.05). The positive rates of 534 blood donors are 22.1% (118/534) and 21.9% (117/534) respectively. Another evaluation on 974 serum samples in National Taiwan University Hospital showed that the positive rate was 27.2% (265/974) by Ausria II-125 (RIA. Abbott Lab.) and that of NIPM EIA Kit was 27.4% (267/974). The undetectable rate and false positive rate of NIPM EIA Kit were 0.41% (4/974) and 0.62% (6/974) respectively. In comparison with four other kind of commercial EIA Kits, the results of NIPM EIA Kit were satisfactory also. We have scaled up the reagent preparations for the kit, except the antibody coated microtiter plate preparation. At the end of March 1985 we will supply 850 EIA kits for the Development Center for Biotechnology for their hepatitis B vaccine production program.

Hepatitis B Surface Antigens↗
PubMed1984

Preparation of enzyme immunoassay kit for the detection of hepatitis B surface antigen.

An attempt was made to prepare enzyme immunoassay (EIA) kit for the detection of hepatitis B surface antigen (HBsAg). The diagnostic reagents were prepared by the 'sandwich" principle in which polystyrene microtiter plates were used. Two-step glutaraldehyde coupling procedure was used for the preparation of antibody-peroxidase (horseradish) conjugate. Purified anti-HBs IgG fraction of guinea pig antisera was used for coating plates and for antibody-enzyme conjugate preparation. This method had been compared with Ausria II-125 (RIA, Abbott Lab.) in 423 government employees. Among 80 (18.9%) positive results six were inconsistent reactions by Ausria II-125. Only one of the six positive specimens was repeatedly positive and was confirmed by Auszyme II (EIA, Abbott Lab.). 14 out of the 343 (81.1%) negative results were positive reactions detected by Ausria II-125. A half of them was still negative detected by Auszyme II. The lowest detectable dose of our EIA, in which the Hepatitis Sensitivity Panel-5 (Abbott Lab.) was used and incubation period was set for 2 hr., was 3.2 ng/ml for ad subtype of HBsAg. While that of Auszyme II was 0.93 ng/ml.

Animals↗
PubMed1979

Dynamic changes of horse serum T-globulin immunization with snake venoms, tetanus and diphtheria toxoids.

In course of immunizing horses with snake venoms, tetanus and diphtheria toxoids, a new serum component, T-globulin, was formed and migrated between the beta- and gamma-globulins. The T-globulin content was parallel with the antibody titre after the middle course of immunization. There were many components in snake antivenin and T-globulin was composed of most of those components. The components of diphtheria T-globulin were the same as those of crude antitoxin and tetanus T-globulin except one precipitin.

Animals↗
PubMed1978

Purification of Bungarus multicinctus antivenin by affinity chromatography.

Bungarus multicinctus horse antivenin was purified by ammonium sulfate fractionation, gel filtration on Sephadex G-200 column, and affinity chromatography. The specific neutralizing capacity of the antibody preparations showed 2.21-fold, 3.12-fold, and 29.6-fold higher than that of the crude antivenin, respectively.

Animals↗
PubMed1975

The life cycle of Echinoparyphium ralphaudyi sp. n. (Trematoda: Echinostomatidae).

The life cycel and morphology of Echinoparyphium ralphaudyi sp. n. is described. Natural infections were found in Bulinus truncatus from Egypt, Ethiopia, and the Yemen Arab Republic, and later in B. forskalii and B. sericinus from Ethiopia. Sporocysts develop near the places of miracidial entry into the snail (the head-foot region, mantle edge, pseudobranch, and antennae). Rediae occur mainly in the ovotestis and in tissues anterior to the liver. The first cercariae are released 24 days postexposure. Metacercariae encyst in various freshwater snails and are localized in the pericardial sac and the posterior part of the kidney. Adult worms live in the small intestine of a variety of experimental animals: hamsters, rats, mice, chicks, ducklings, pigeons, and finches.

Animals↗
PubMedDate not supplied

Zellweger syndrome: report of one case.

Zellweger syndrome is a fatal autosomal-recessive hereditary disease characterized by the absence of peroxisomes in liver and kidneys. The absence of peroxisomes results in impairment of many metabolic pathways, especially beta-oxidation of very long chain fatty acids (VLCFAs). We report a case of a three-month-old male infant with facial dysmorphism, hypotonia, psychomotor retardation, and hepatomegaly. He had an elder brother with the same facial features and hypotonia who died of hepatic failure at four months of age. Biochemical studies revealed elevation of blood pipecolic acid and VLCFAs, compatible with peroxisomal disorder. Electron microscopy of liver biopsy revealed absence of peroxisomes. Zellweger syndrome was diagnosed. Because this syndrome is usually fatal in early life, genetic counseling and prenatal diagnosis are crucial.

Biopsy↗