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Biomedical subjects

H Endo

Publications and source records attributed to H Endo.

At least 505 records · Page 28Linked to original sources
PubMed1982

[Clinical management of acute lymphocytic leukemia in adults. 1. Treatment of acute lymphocytic leukemia with VP (vincristine, prednisolone)--DVMP (daunorubicin, vincristine 6-mercaptopurine, prednisolone) regimen].

Eighteen patients with acute lymphocytic leukemia (ALL) were treated with VP (vincristine, prednisolone) followed by DVMP (daunorubicin + vincristine + 6-mercaptopurine + prednisolone) regimen (VP-DVMP regimen). Patients were all previously untreated. Complete remission (CR) was obtained in 11 of 18 patients (61.1%,) by VP alone and 4 patients, by VP-DVMP. The time required for CR varied from 14 to 60 days with a median of 28 days. The duration of CR and survivals in responders were from 1.2 to 42.3 + months with a median of 24.0 months. The hematological toxicities in VP-DVMP regimen were lower than those in NVP (neocarzinostatin + vincristine + prednisolone) and NVMP (neocarzinostatin + vincristine + 6-mercaptopurine + prednisolone) regimens.

Adolescent↗
PubMed1982

[Endometrial cytology by endocyte and by Masubuchi's aspiration technic --a comparative study--].

Lately there is a tendency for an increase in the incidence of endometrial cancer both in this country and other industrialized countries such as the United States. The routine cervicovaginal smear method is generally poor for the detection of endometrial carcinoma as compared to that used for the diagnosis of early cervical neoplasms. The principal reason for the poor detection rate is that the cells are not obtained directly from the lesion as is the case with disease of the cervix. Various techniques for obtaining endometrial cells directly from the lesions have been described, but none has enjoyed wide acceptance by the clinicians. The patients for this study consisted of 138 women who were referred to us for diagnostic curettage. We employed the new disposable device, the Endocyte, and Masubuchi's endometrial aspiration and compared the results. After obtaining specimens with both methods curettage was performed on all patients. In addition, the Endocyte was employed on 8 patients with endometrial carcinoma in another hospital, and the following results were obtained. 1. With the Endocyte it is possible to obtain a larger volume of cells than by aspiration. 2. Although a certain amount of difficulty is encountered in the insertion of the Endocyte in some cases, it is much easier than the brush method used heretofore. 3. No significant difference in the amount of bleeding after insertion could be observed between the two methods. 4. In patients with bleeding in the endometrium and pyometra it is easier to obtain specimens from the endometrium with the Endocyte than by aspiration. 5. With the Endocyte contamination of the cells of the cervix was less than after aspiration. 6. Although pain during collection of cells with the Endocyte was somewhat stronger than during aspiration, analgesics were not necessary in any one of the cases. 7. The Endocyte was used on 13 patients with endometrial carcinoma, and all specimens were positive for carcinoma. From the results of our study we conclude that for endometrial cytology the Endocyte is as useful as the aspiration method. Furthermore, it should pose no difficulty to the gynecologists to use the Endocyte because the Endocyte resembles IUD structurally.

Adult↗
PubMed1981

The synthesis of ribosomal 5 S RNA in cultured hamster cells during the inhibition of protein synthesis.

Ribosomal 5 S RNA synthesis in Chinese hamster V79 cells treated with an inhibitory antibiotic of protein synthesis, cycloheximide, was quantitated by hybridization of RNA preparations with plasmid ColE1-pSC101 DNA carrying Xenopus 5 S DNA. In V79 cells, cycloheximide produced a notable decrease in the production of the higher molecular weight ribosomal RNA (rRNA) fraction, whereas the accumulation of both 5 S RNA and polyadenylate-containing messenger RNA was much less affected. When the cellular protein synthesis was inhibited by over 85% of the control, accumulation of 5 S RNA and of rRNA was respectively 40-50% and about 10% of the control. The size distribution analysis of RNA species revealed that 5 S RNAs obtained from V79 cells after treatment with 0.1 microgram/ml or 2.0 microgram/ml cycloheximide were indistinguishable from control. Inhibition of precursor uptake into 5 S RNA by cycloheximide was apparently phase-specific during the cell cycle and was 44-67% and 78-85% of the control for the S and the M phase, respectively. The responses of RNA polymerase III activities to cycloheximide in isolated nuclei from each phase of the cell cycle correlated very well with those of precursor uptake into 5 S RNA observed in vivo, while total solubilized RNA polymerase activities showed no inhibition by the drug at any phase of the cell cycle. Cytosine arabinoside, a specific inhibitor of DNA synthesis, did not cause any decrease in the cellular level of 5 S RNA.

Animals↗
PubMed1981

Induction of chromosomal aberrations and 8-azaguanine-resistant mutations by aryldialkyltriazenes in cultured mammalian cells.

Inducibility of chromosomal aberrations, cell survival, and mutation to 8-azaguanine (8AG) resistance in cultured V79 cells by 1-phenyl-3,3-dimethyltriazene (PDMT), 1-phenyl-3,3-diethyltriazene (PDET), and 1-(pyridyl-3)-3,3-diethyltriazene (PyDET) were examined with or without metabolic activation. Chromosomal aberrations were induced in a dose-dependent manner by all 3 triazenes in a direct treatment for 24 h. Chromosomal aberrations were also induced by PDMT with metabolic activation system for 3 h, and little differences in the incidences were observed compared with those obtained by a direct treatment. All 3 triazenes were slightly mutagenic in a direct treatment for 24 h. In metabolic activation experiments, however, PDMT and PyDET were highly mutagenic. The mutagenicity, when compared with the cytotoxicity, was significantly higher in a metabolic activation system than in a direct treatment.

Animals↗
PubMed1981

Capacities of mouse embryo cells for induction of unscheduled DNA synthesis by 4-nitroquinolone 1-oxide in primary culture systems.

Because of 4-nitroquinoline 1-oxide (4-NQO) selectively induces lung tumors in offspring of ICR/Jcl inbred mice, unscheduled DNA synthesis (UDS) induced by 4-NQO was investigated with use of confluent primary cultures of cells from six different organs of ICR/Jcl mouse embryos. The UDS level of lung-derived cells (lung cells), as measured with hydroxyurea-resistant incorporation of [3H]thymidine into cellular DNA in the presence of 4-NQO, was at least threefold lower than levels in cells from skin, intestine, kidney, liver, and brain, when compared at equitoxic doses of 4-NQO of equimolar binding of 4-NQO to DNA Autoradiographic studies also revealed that the percentage of UDS-positive nuclei in lung cells was about one-fifth of other cell types. Cytotoxic examinations showed that the lung cells were the most sensitive to the lethal action of 4-NQO. These results suggest that the capacity of cells from the target organ to repair 4-NQO damage is intrinsically much lower than the capacity of cells from nontarget organs.

4-Nitroquinoline-1-oxide↗
PubMed1980

Vitamin D3 metabolites and PTH synergistically stimulate bone formation of chick embryonic femur in vitro.

Bone remodelling depends on a balance formation and resorption. Little is known of the biological factors involved in bone formation, whereas there is much evidence that physiological factors, such as parathyroid hormone (PTH) and active metabolites of vitamin D3, influence resorption. Cells responsible for osteogenesis and and osteoclasis occur in close proximity, suggesting that both might be controlled by the same physiological factors in the micro-environment of the body fluid. To clarify the factors stimulating bone formation or accelerating osteogenic cell activity, we have examined the effects of vitamin D3 metabolites and PTH on bone formation in vitro. We report here that combinations of those metabolites especially 1 alpha,25-(OH)2D3 and 24R,25-(OH)2D3, directly stimulate calcification on bone synergistically with PTH.

Animals↗
PubMed1980

Incontinentia pigmenti achromians (Ito).

A patient with incontinentia pigmenti achromians was reported. She displayed other abnormalities such as a saddle nose, malformed auricles, growth retardation, convulsions and abnormal EEG. Histopathologically, a partial decrease in melanin granules in the basal layer and the presence of melanocytes which showed a weak dopa reaction were the main findings. The hypofunction of melanin production was detected by electron microscopy.

Abnormalities, Multiple↗
PubMed1980

Growth stimulative effect of parathyroid hormone, calcitonin and N6,O2'-dibutyryl adenosine 3';5'-cyclic monophosphoric acid on chick embryonic cartilage cultivated in a chemically defined medium.

PTH (0.5 unit/ml) and CT (0.5 unit/ml) strongly stimulated the in vitro growth of the chick embryo femur in terms of elongation and dry weight increase as well as an increase in the protein, hexosamine, hydroxyproline, DNA and RNA content of the femur. A synergistic effect was observed when PTH and CT were simultaneously added to the medium, suggesting that the mechanism of the growth stimulating effect of one of these hormones might be different from that of the other. The exposure of PTH to the femur caused an increase in cyclic AMP content. The direct addition of dibutyryl cyclic AMP (1 mM) to the medium mimicked the effect of PTH on the cartilage. PTH, therefore, seems to stimulate the growth of cartilage via the increase in cyclic AMP content of the femur. CT had no effect on the cyclic AMP content of the femur.

Animals↗