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Biomedical subjects

H Eberle

Publications and source records attributed to H Eberle.

At least 73 records · Page 4Linked to original sources
PubMed1971

DNA replication and proteins that bind to DNA.

Cells of Escherichia coli 15T(-) were exposed to nalidixic acid or starved for thymine to inhibit DNA synthesis while protein synthesis was allowed to continue. These treatments are known to allow subsequent DNA synthesis to continue for many hours in the absence of protein synthesis. The proteins extracted from these cells that bind to DNA were examined by DNA-cellulose chromatography and disc-gel electrophoresis. One or two species were found to be absent, or to be present in greatly reduced amounts, under these conditions.

Amino Acids↗
PubMed1970

Concerning the influence of integrated episomes on chromosomal replication in Escherichia coli.

Two strains of Escherichia coli K12, C600, and DG163, in which the origins and directions of DNA replication had been mapped previously, were made lysogenic for lambda and lambdai434. The origins of replication in these lysogenic strains were located with respect to the prophage location by chromosome alignment and subsequent hybridization of (3)H-labeled lambda vir DNA with newly replicated and "old" DNA. The results obtained are consistent with similar origins of replication observed in the nonlysogenic strains. These findings suggest that DNA replication in these cells is under the control of the E. coli chromosomal replicon and is independent of the presence of the lambda replicon as predicted by the Jacob, Brenner, and Cuzin model of DNA replication.

Amino Acids↗
PubMed1969

Relation of the segregative origin of chromosome replication to the origin of replication after amino acid starvation.

Cultures of Escherichia coli 15T(-) and K-12 were labeled with (3)H-thymine before, during, and after amino acid starvation. The number of labeled segregating units was measured by autoradiography of microcolonies derived from the labeled cells. In both strains, labels inserted before starvation and during starvation appeared to segregate as if incorporated into the same polynucleotide strands. However, labels inserted during and after starvation segregated as if incorporated into different polynucleotide strands. In view of previous data, it was concluded that replication after amino acid starvation originates from the region of the chromosome which serves as the origin for replication during normal growth and division.

Autoradiography↗