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Biomedical subjects

H E Schaefer

Publications and source records attributed to H E Schaefer.

At least 127 records · Page 7Linked to original sources
PubMed1982

Cytochemical investigations of phagocytes in thyroid gland cysts.

Certain similarities in the cytoplasmic structure could indicate a histogenetic affinity between thyrocytes and pigmented macrophages in thyroid glands. On the other hand, the monocytogenic origin of macrophages in other organs in the body contradicts this assumption. We investigated these cells in smears of thyroid aspirates as well as in imprints, frozen sections and paraffin-embedded sections of freshly removed thyroid glands, using several cytochemical reactions and the usual panoptic stains. With the sodium-fluoride-inhibited alpha-naphthyl acetate esterase and tartrate-inhibited acid phosphatase reactions, the pigmented macrophages exhibited enzyme cytochemical patterns corresponding to those of blood monocytes or monocytogenic histiocytes but differing definitely from those of thyrocytes. Furthermore, we observed macrophages that exhibited a clearly monocytoid nuclear form and chromatin pattern in the interstitial space of the thyroid gland. These findings practically exclude a derivation of thyroidal pigmented macrophages from follicular epithelial cells and positively underline their derivation from blood monocytes.

Acid Phosphatase↗
PubMed1981

[Ring-shaped inclusions in an uncommon variety of acute myeloid leukemia (author's transl)].

In cells of an acute, predominantly promyelocytic differentiated leukemia ring-shaped inclusions could be demonstrated by light- and electron-microscopic examinations. The patient showed good response to aggressive chemotherapy with complete remission. It is discussed, how far the presence of these morphological characteristics will have any bearing on the classification of this variety of leukemia.

Adolescent↗
PubMed1981

[Chronic lymphoproliferative disorder resembling hairy-cell leukemia (author's transl)].

Seven patients are presented with a chronic lymphoproliferative disorder characterized clinically by splenomegaly, no or discrete lymphnode enlargement, and a varying degree of cytopenia. In blood and bone-marrow smears lymphoid cells of "hairy" appearance are demonstrable which may contain tartrate-resistant acid phosphatase. The finding of a nodular bone-marrow infiltration without fibrosis as well as that of a nodular infiltration of the spleen originating in the white pulp are incompatible with the diagnosis hairy-cell leukemia and place the disease near to chronic lymphocytic leukemia (CLL) or leukemic immunocytoma respectively. A detailed cytologic and cytochemical examination of the infiltrating cells shows deviations from the typical enzymatic pattern of hairy cells and from known enzymatic constellations in CLL and related lymphoproliferative disorders. Thus, we are dealing with an intermediate form, difficult to classify, the separation of which nevertheless seems to be important for therapeutical reasons.

Acid Phosphatase↗
PubMed1980

[Kaposi's sarcoma (author's transl)].

Kaposi's sarcoma of the skin and an aplastic syndrome occurred together in a 51-year-old patient. Macroscopically livid papules and nodules were observed. Histomorphologically endotheliomatous cell proliferation with signs of infiltrative growth was found. Because of the aplastic pancytopenia cytostatic treatment of the Kaposi sarcoma was contraindicated. The patient finally died of vascular failure with haemorrhagic diathesis being manifest. Syntropy of Kaposi's sarcoma with malignant haematological diseases is known. However, association with aplastic anaemia has not been observed so far. The pathogenesis of Kaposi's sarcoma is unknown.

Anemia, Aplastic↗
PubMed1980

[Sézary syndrome (author's transl)].

Morphological demonstration of the typical Sézary cell in peripheral blood confirmed the diagnosis of Sézary syndrome in a 64-year-old patient with generalized erythrodermia and typical histopathological skin changes. Enzymes and immunocytological membrane characteristics of Sézary cells changed in the course of the disease after cytostatic therapy. Polychemotherapy clinically led to transitory complete regression of skin changes. Reviewing the few published investigations it is found that the morphologically sufficiently defined Sézary cells are apparently not uniform cytochemically and immunocytologically in different patients.

Autopsy↗
PubMed1980

The significance of lectin receptors in the kidney and in hypernephroma (renal adenocarcinoma).

Normal kidney tissue as well as hypernephromas were examined histochemically for the occurrence of lectin receptors. FITC- and rhodamine-labeled peanut agglutinin (PNA) and Ricinus communis agglutinin (RCA) were used for labeling different carbohydrate residues which may be of interest in the evaluation of the histogenesis of hypernephromas and possibly for concepts regarding the immunotherapy of these tumors. By using fluorescence microscopy, the receptor for PNA was found on the epithelial cells of the thin limb, distal convoluted tubules, and the collecting ducts. These binding sites occurred in a free as well as in a sialic-acid-substituted form and were mainly exposed on the luminal surface of the epithelial cells. They were absent, however, in the proximal convoluted tubules. This finding contrasts with the demonstration of RCA receptors in the brush border of these tubules. Moreover, RCA reacted with the epithelial cells of all tubules. The hypernephromas showed a wide range in the distribution and the quality of the lectin receptors, which were localized within the cytoplasm as well as in the cell membrane of the tumor cells. As demonstrated by our histochemical investigations, these kidney neoplasms may originate from any part of the tubules, and not only from the epithelial cells of the proximal convoluted tubules, as was suggested by earlier findings that employed antibodies against the brush-border antigens. In addition, the demonstration of sialic-acid-substituted and, in particular, of free PNA-receptors, which represent the immunodominant group of the Thomsen-Friedenreich antigen, may supply useful information on an immunotherapy concept.

Adenocarcinoma↗
PubMed1980

Sézary syndrome: immunocytological and cytochemical variability of Sézary cells.

The characteristic large cells in the blood of a patient with Sézary syndrome underwent immunocytological and cytochemical changes during polychemotherapy, which caused transient regression of skin lesions. Tartrate-resistant acid phosphatase was demonstrable in a few cells only after chemotherapy; initially, only 2% T cells and 26% B cells could be demonstrated in the blood by immunocytological methods; after cytostatic therapy, 35% T cells but no B cells were detected.

Acid Phosphatase↗