Biomedical subjects

H E Jensen

Publications and source records attributed to H E Jensen.

At least 19 recordsLinked to original sources

PubMed1992

The occurrence of fungi in bovine tissues in relation to portals of entry and environmental factors.

The occurrence of fungi in tissue specimens from 72 cattle was examined by culture, histopathology and indirect immunofluorescence staining (IIF). Groups of 12 animals each had been fed either concentrate or roughage and had been housed either in tie stalls, on slatted floors or on deep bedding. Specimens were obtained from the lung, omasum and Peyer's patches of the ileum and corresponding lymph nodes. Both hyphae and spores were made visible by IIF and by combination of IIF, morphology and conventional staining it was possible to differentiate between aspergilli and zygomycetes. In the lungs, aspergilli were detected at the same rate by morphology and IIF, whereas zygomycetes were found nearly twice as often by IIF than by culture. Fungi in pulmonary tissue were most frequent in cattle tied or kept on deep bedding (P < 0.01) as assessed by IIF. Within lymph nodes only spores were found, and Aspergillus fumigatus was the predominant species. Lesions devoid of fungi, especially ulcerations, were observed on the edges of the largest omasal laminae with a notable preference for the aboral third. The localization and histopathology suggested a reflux of acid abomasal contents to be the pathogenic principle. Granulomas with centrally located plant material were found more frequently in cattle fed roughage than in cattle fed concentrate (P = 0.01) and were differentiated from mycotic granulomas.

Animal Feed↗

PubMed1992

Disseminated histoplasmosis in a badger (Meles meles) in Denmark.

We report the first case of disseminated histoplasmosis in an animal in Scandinavia. Yeast cells compatible with those of Histoplasma capsulatum var. capsulatum were found in the skin, liver, spleen, a kidney, and a lymph node of a wild badger (Meles meles). The diagnosis was confirmed by electron microscopy and immunofluorescence staining of the yeast cells in tissue sections.

Animals↗

PubMed1992

Aspergillus fumigatus fungaemia and myocarditis in a patient with acquired immunodeficiency syndrome.

Necrotizing myocarditis due to Aspergillus fumigatus was a contributory cause of death in a patient with acquired immunodeficiency syndrome and non-Hodgkin lymphoblastic malignant lymphoma of the Burkitt type. A transient remission of the lymphoma had been obtained by cytostatic treatment. A. fumigatus was isolated from blood two weeks before death, but myocarditis was not diagnosed until autopsy.

Acquired Immunodeficiency Syndrome↗

PubMed1992

Aspergillus and aspergillosis.

Explore the source record for details and available documents.

Animals↗

PubMed1991

Acute disseminated Aspergillosis in a cow with special reference to penetration and spread.

Acute disseminated aspergillosis caused by Aspergillus fumigatus is reported in a 4-year-old cow given broad spectrum antibiotics and anti-inflammatory therapy. All stomach compartments were infected and hyphal invasion of gastric blood vessels was the probable source of generalization to the liver, lung and kidneys. Throughout the Peyer's patches of the jejunum there were acute necrotic foci with Aspergillus hyphae and similar foci occurred in the corresponding mesenteric lymph nodes. This points to the Peyer's patches being a portal of entry for A. fumigatus to the lymphatic system of the intestine.

Abomasum↗

PubMed1991

Bovine mycotic abortion--a comparative study of diagnostic methods.

The agreement between three diagnostic methods (mycology, morphology, and immunofluorescence staining) was compared two by two in elucidating the aetiological diagnosis in 52 cases of bovine mycotic abortion. Agreement between the three methods was expressed by the kappa coefficient (kappa), and ranked as follows: mycology and immunofluorescence staining (kappa = 0.48); morphology and immunofluorescence staining (kappa = 0.40); mycology and morphology (kappa = 0.28). A. fumigatus was confirmed to be the main cause of bovine mycotic abortion with members of the zygomycetes (order Mucorales) being the second most important group. In two aborted placentas a Candida species was found to be the cause of mycotic placentitis.

Abortion, Veterinary↗

PubMed1991

Experimental candidosis in pregnant mice.

Pregnant mice were challenged intravenously with doses of 1 x 10(3)-1 x 10(7) Candida albicans blastospores, and from postmortem histopathology it was found that C. albicans had a propensity for localization in the murine placenta. At the highest dose, blastospores, hyphae and pseudohyphae were randomly located in the foeto-placental units, whereas proliferation of fungi at lower doses started in the rim of the placental disc, after which it spread along Reichert's membrane and/or the splanchnopleure, eventually attacking the foetal skin. These findings are analogous to our previous observations on the nature of Aspergillus fumigatus infection in pregnant mice.

Animals↗

PubMed1990

[Iatrogenic biliary tract injuries].

From 1984 to 1986 The Medico-Legal Council was heard concerning four cases of iatrogenic lesions of the common bile duct during cholecystectomy. The Council's statements show that lesions of the common bile duct or the common hepatic duct during otherwise uncomplicated, elective cholecystectomy are considered surgical malpractice and not accidental mishaps.

Cholecystectomy↗

PubMed1990

[Measurement of the quality of life as a element in assessing therapeutic effects in essential arterial hypertension. Methodologic aspects].

The quality of life has become an important element in assessment of medicinal treatment. As an independent expression, the quality of life covers the human disappointments which a medical disease may involve ("discomfort") whereas the clinical dysfunction caused by the disease is covered by the expression "disability". In the treatment of essential arterial hypertension, the clinical symptoms of the disease are few or none ("asymptomatic hypertension"). In an investigation of 303 patients in general practice for arterial hypertension, the significance of the frequency of dosage of metoprolol (Seloken) was assessed by means of measurement of the quality of life by the General Health Questionnaire (GHQ), a questionnaire which contains components such as fatigue, sleep, anxiety and depression. No advantages in the quality of life were found in administering antihypertensive drugs once or twice daily. From the methological point of view, the following conditions were found which should be taken into consideration in future investigations in this field: The GHQ was found to be easy for the patients to employ. This is important as the quality of life is a personally experienced dimension. The questionnaire correlated adequately with the visual analogue scales. Approximately 1/3 of the patients had reduced quality of life at the commencement of the investigation as assessed by GHQ. A multiple regression analysis revealed that the reduced well-being was due to social, life events and side effects. It is therefore recommended that these aspects should be described when patients commence a course of medicinal treatment i.e. the social life events and side effects of the medicine in connection with the actual subjective dimension of quality of life.

Adult↗

PubMed1990

[Cecostomy can not be recommended as a routine method in the treatment of acute left-sided obstructive colon cancer].

During a period of 17 years, 135 patients with acute left-sided obstructive cancer of the colon were admitted to two hospitals in the County of Copenhagen. Primary decompression by means of coecostomy was performed. The plan was to perform resection of the tumour after some weeks and colocolic anastomosis and closure of the coecostomy either spontaneously or operatively after some months. The total mortality was 24%. A total of 84 patients (62%) completed the planned therapeutic course, 21 without complications. Eighteen patients survived with permanent coecostomies. The mortality in this material was similar to those in materials where other therapeutic regimes were employed. The prolonged therapeutic programme and considerable morbidity thus do not result in reduction in the mortality. The authors cannot therefore recommend primary decompressive coecostomy. This method can only be recommended as an emergency measure under local anaesthesia for patients in such poor general condition that other forms of treatment are not possible.

Adult↗

PubMed1990

A murine model for the study of the impact of Aspergillus fumigatus inoculation on the foeto-placental unit.

Pregnant female BALB/c mice were inoculated intravenously with Aspergillus fumigatus conidia in different concentrations (1 x 10(1)-1 x 10(8)) on day 10 of pregnancy. Pregnancy was confirmed by the presence of murine alpha-fetoprotein (m-AFP) in the maternal circulation. Conidia inoculation of 1 x 10(3) and greater significantly (p less than 0.0001) resulted in abortion or placental lesions. Uterine cell infiltrations were recognized in 75% of the mice which aborted following the inoculation of 1 x 10(3) conidia or more. In the placental lesions of mice inoculated with 0.5-1 x 10(8) conidia, hyphae, haemorrhage, thrombosis, necrosis, calcification, and inflammation were regular findings. Histopathology of other maternal organs revealed that extrauterine organ lesions were closely correlated with the dose of conidia inoculated, and the following pattern of reaction was found: liver greater than lung greater than kidney greater than brain greater than heart.

Abortion, Veterinary↗

PubMed1990

Intestinal and pulmonary mycotic lymphadenitis in cattle.

Among 4877 slaughtered cattle with tuberculosis-like lesions in lymph nodes, 94 cases (1.9 per cent) revealed fungal hyphae on histopathological examination. The survey period was 12 years and most of the affected animals (greater than 77 per cent) were beef cattle. Affected nodes were mesenteric in 84 cases (89.4 per cent), mediastinal and/or bronchial in seven cases (7.4 per cent), and in three cases (3.2 per cent), both mesenteric and mediastinal nodes were affected. The incidence of mycotic lymphadenitis was unrelated to year of study or season. Eighty-two of the cases were re-examined histologically and immunohistochemically. All lesions were granulomatous in nature and, in 26 cases, eosinophilic asteroid bodies (rosette formation) around hyphae were found. In 75 cases, immunofluorescence staining identified the agent as a zygomycete, probably Absidia corymbifera and, in one case, there was a concurrent infection with a Candida species. In seven cases that did not react with the antibodies employed, a diagnosis of zygomycosis was suggested on the basis of hyphal morphology. Hyphae of Aspergillus spp. were not found in any of the lesions. A concurrent fungal and mycobacterial infection (M. avium) was diagnosed in one case. Thus, zygomycetes are the main cause of macroscopically apparent mycotic lymphadenitis, a sporadic disease most probably caused by feeding with mouldy food stuffs.

Animals↗

PubMed1989

[A prospective controlled study of vagotomy in the treatment of duodenal ulcer. Results after 11-15 years].

Clinical Trials as Topic↗

PubMed1989

Immunofluorescence staining of hyphae in the histopathological diagnosis of mycoses in cattle.

An immunofluorescence staining system was developed for the identification of mycelial fungi causing systemic mycoses in cattle. Antisera to Absidia corymbifera, Aspergillus fumigatus, Aspergillus flavus, and Aspergillus niger were raised in rabbits. Tissue sections of experimentally infected mice were used to determine the specificity of immune staining with antisera that had been absorbed with heterologous antigens. When 46 lesions from 22 cattle with systemic mycosis were studied, the infecting agent could be classified as a zygomycete, possibly A. corymbifera, in 18 lesions (15 cattle), A. fumigatus in 10 lesions (7 cattle) and both a zygomycete and A. fumigatus in a single lesion from one animal. Diagnosis was established by light microscopy in 13 (72%) of the 18 lesions classified as zygomycosis. Conversely, a dual infection with both a zygomycete and an Aspergillus species was suspected on the basis of morphology in nine of the 10 lesions reacting exclusively and uniformly with anti-A. fumigatus antiserum. A. fumigatus may be a more frequent aetiological agent of systemic mycosis in cattle than hitherto recognized on the basis of light microscopy and mycological culture.

Animals↗

PubMed1989

Pulmonary mycosis in farmed deer: allergic zygomycosis and invasive aspergillosis.

During 1988, pulmonary mycosis was diagnosed in four of 116 farmed deer examined on suspicion of tuberculosis. The histopathology showed allergic bronchopulmonary mycosis in a red deer (Cervus elaphus) and the agent was identified as a zygomycete, probably Absidia corymbifera, by immunofluorescence staining. Three fallow deer (Dama dama) had invasive necrotizing mycotic pneumonia and progressive exudative mycotic alveolitis caused by Aspergillus fumigatus. In the red deer, weakness due to paratuberculosis had probably promoted the mycotic infection. The three fallow deer were bred on another farm, where predisposing factors included mouldy straw and incorrect management.

Animals↗

PubMed1989

Prospective controlled vagotomy trial for duodenal ulcer. Results after 11-15 years.

A prospective, randomized, controlled trial was conducted to compare truncal vagotomy and drainage (TV), selective vagotomy and drainage (SV) and parietal cell vagotomy (PCV) as elective treatment for duodenal ulcer. Between 11 and 15 years after operation, 248 patients were available for study of the recurrent ulceration rate by a life table method, and 197 patients could be studied with regard to postvagotomy symptoms. The recurrent ulcer rates were 28.5% for TV, 37.4% for SV, and 39.3% for PCV. These differences were not statistically significant. The incidence of severe postvagotomy symptoms was as follows: dyspepsia, 18.4% for TV, 20.5% for SV, 8.6% for PCV; dumping, 5.9% for TV, 19.6% for SV, 2.2% for PCV; diarrhea, 9.8% for TV, 11.8% for SV, 4.4% for PCV. The incidence of severe dumping was significantly less frequent among the PCV patients than the SV group. The differences did not reach statistical significance in any of the other groups. There was no significant difference in the Visick gradings among the three groups either before or after treatment of the failures. About two thirds of the patients in each group were finally satisfied with their operation, often after second operations or prolonged medical treatment. It is concluded that none of the three forms of vagotomy can be recommended as the standard operative treatment of duodenal ulceration.

Clinical Trials as Topic↗

PubMed1989

Localization of plasmin on the surface of the human trophoblast.

The present study demonstrates the localization of plasmin on the surface of the microvilli of the human placenta using indirect immunofluorescence. This localization might explain why serum levels of fibrin/fibrinogen degradation products are higher in the uterine vein than in the uterine artery. In view of the previous finding of pregnancy zone protein on the trophoblastic surface and the affinity between plasmin and pregnancy zone protein, it is suggested that pregnancy zone protein immobilizes plasmin on the trophoblastic surface.

Female↗

PubMed1989

Mycosis in the stomach compartments of cattle.

During a period of 22 months, 9.7% (23 out of 238) of the cattle necropsied at the Department of Veterinary Pathology had mycotic affections in the stomach compartments. The various stomach compartments were affected in the following pattern: omasum: 15 cases, rumen: 10 cases, reticulum: 8 cases and abomasum: 6 cases. In 5 of the animals mycotic affection was also found in other organs of which the liver was most often involved. Out of the 23 cases described, a primary diagnosis of mycosis was only established in 5 cases. In the remaining 18 cases the mycotic infection was regarded as being of a minor importance in the total necropsy result. Macroscopically and histologically the mycotic processes could be grouped into acute, subacute or chronic affections. In 20 cases only one type of inflammation was present, in the remaining cases combinations were found. Identification of the infective agent was performed by immunological staining of hyphal structures in tissue sections. Members of the Zygomycetes were found to predominate as causative fungi. Aspergillus fumigatus was found to have a propensity for infecting the terminal gastric compartments, whereas the Zygomycetes were found regularly in all compartments. Candida spp. were only found in 1 case. Predisposing factors for developing systemic mycosis are discussed. Among these especially the use of antimicrobial drugs, other diseases, metabolic disturbances and stressors in the post parturient period seem to be important.

Animals↗