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Biomedical subjects

H E Enesco

Publications and source records attributed to H E Enesco.

At least 37 records · Page 2Linked to original sources
PubMed1978

Radioautographic study of the effect of malononitrile dimer on RNA synthesis in mice.

The drug malononitrile dimer (1,1,3-tricyano-2-amino-1-propene) was administered intraperitoneally to groups of mice at 20 mg/kg body weight, either as a single injection or as chronic 2-month injections. To study the effect of malononitrile dimer on RNA synthesis in the brain, it was necessary to choose a precursor that would pass the blood-brain barrier. Animals were injected with 14C-formate, then sacrificed 1 h later. RNA synthesis in the brain, liver and kidney of the drug-injected and control animals was determined by the amount of 14C-formate incorporated into newly synthesized RNA. A new method involving the comparison of RNase-extracted with non-extracted slides is described for determining the proportion of 14C-formate incorporated into RNA radioautographically. The results show that RNA synthesis was significantly increased as a result of both single and chronic malononitrile dimer injections in all tissues studied. In most cases, malononitrile dimer nearly doubled the percentage of 14C-formate incorporated into newly synthesized RNA. The effect of the chronic injections was most pronounced in the brain. The mechanism of malononitrile action on RNA synthesis are mediated by thyroxine is discussed.

Animals↗
PubMed1976

Effect of malononitrile dimer on RNA concentration of neurons as demonstrated by azure B staining.

Malononitrile dimer was administered to mice by single or by chronic (40 day) injections. The concentration of RNA in neurons of the brain was determined on histological sections by means of azure B staining. The nucleolus and Nissl substance of the several types of large neurons studied had a significantly higher concentration of RNA in the drug-injected than in the control animals.

Animals↗
PubMed1967

RNA and memory: a re-evaluation of present data.

Studies on the beneficial effects of yeast RNA treatment on learning and memory in senile patients and in animal experimentation are reviewed in this paper. Experimental evidence is cited showing that yeast RNA is not incorporated into brain tissue and does not increase brain synthetic activity. Thus the therapeutic effects of RNA treatment cannot be explained in direct relation to the hypothesis that RNA within the brain encodes memory information. Evidence for stimulant action of RNA, mediated by uric acid, its primary metabolite, is presented as the basis for an alternative theory which would explain RNA action in pharmacological terms.

Humans↗