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Biomedical subjects

H Donat

Publications and source records attributed to H Donat.

At least 55 records · Page 3Linked to original sources
PubMed1983

[Detection of organ-specific and organ non-specific autoantibodies in the serum of sterile couples].

Sera from 150 infertile and 50 fertile couples were examined for organ-specific and non-organ-specific antibodies by indirect immunofluorescence tests on unfixed cryostat sections. Adrenocortical antibodies to zona fasciculata cells were found in 4 females and 2 males of infertile couples. Parietal cell antibodies were demonstrated in 4 females and 2 males of infertile couples. Parietal cell antibodies were demonstrated in 4 female and 3 male sera. Antibodies to mitochondria could be found in 14 fertile and 72 infertile couples. Antinuclear antibodies occurred in 2 fertile and infertile couples. Smooth muscle antibodies were found more often in infertile than fertile couples. Glomerular, skeletal muscle and reticulin antibodies were detected only in infertile couples. The detection of autoantibodies can help to elucidate immune reactions causing infertility.

Adrenal Cortex↗
PubMed1982

[Importance of the spermatozoan ascension test in sterility diagnosis].

Ascension and penetration tests were applied to 60 sterile patients for assessment of spermatozoan migration. Ascension tests were positive in 16 cases and penetration tests in 22. Ten sterile women produced positive responses to either test. Pregnancy rates were high in the cases with positive ascension tests, but highest when both tests were positive. Negative test results were attributable primarily to andrological factors, pathological conditions in the cervix or to impaired tubal patency on account of adhesions.

Female↗
PubMed1981

Investigations into the metabolism of human spermatozoa in relation to penetration capacity.

Using a special chamber, the authors investigated the penetration capacity of spermatozoa of 120 sterile couples in both simple and crossed mucus penetration tests. Moreover, the glycolytic activity and the oxygen consumption of the ejaculates were biochemically determined in an artificial cervical mucus medium. A negative spermatozoan penetration was found to be associated with a markedly reduced aerobic glycolytic activity. It was for low-motility spermatozoa that no penetration was observed in cervical mucus, if the aerobic glycolysis was diminished, but the respiratory activity enhanced. Comparative investigations into the penetration capacity of spermatozoa in an artificial cervical mucus medium permit the detection of causes of male sterility, which exhibit metabolic regulation disturbances of spermatozoa.

Cervix Mucus↗
PubMed1981

Immunological factors in human male infertility: immunopathological studies of testes.

Biopsy specimens of human testes from 190 men of infertile couples were treated with fluorescein-labelled antibodies to demonstrate tissue-bound IgG, IgA, IgM, IgD, C3, and C4. In 24 cases (12.6%) autoantibodies and/or complement could be detected. Most often IgG bound to walls of seminiferous tubules was found. Three men had antibodies or complement in germinal cells. In one case each IgA immunofluorescence was found in spermatids or Leydig cells. The results suggest that autoantibodies to testicular antigens may be implicated in the pathogenesis of some cases of infertility in man.

Adolescent↗
PubMed1981

[Effectiveness of primary and postsurgical hydrotubation (author's transl)].

Hydrotubation is a conservative method to cope with complete or partial tubal occlusion. Hydrotubation was applied postsurgically to 60 women and as primary treatment to another 72 women, all patients suffering from primary and secondary sterility. - Minor complications, including abdominal pain, were recorded from 15 patients. Pregnancy occurred to 13, among them eleven who had received postsurgical hydrotubation.

Fallopian Tube Diseases↗
PubMed1981

[Abnormal obstetric presentation--consequence of defective vestibular function (author's transl)].

The possible presence of a relationship between intra-uterine presentation and vestibular function was studied for this paper. -- Thermic and rotational balance tests as well as tests for vestibulospinal reaction and for spontaneous and provoked nystagmus were applied to 42 children in school age who had been born from breech and transverse presentations and to another 30 children who had been born from vertex presentations. The results seem to support the assumption that normal intrauterine presentation depends on intact condition of the vestibular system.

Breech Presentation↗
PubMed1981

[Correlations between penetration tests and spermatozoal antibodies (author's transl)].

The results obtained from penetration testing of cervical mucus of 60 sterile couples are reported in this paper. Also, the presence of spermatozoal antibodies in the serum of both the female and male probands was detected by means of the immunofluorescence method. --Neither in the females nor males was any relationship detectable between the occurrence of spermatozoal antibodies and the results of penetration testing. Yet, spermatozoal antibodies were found to occur much more often in probands with sterility of unelucidated origin.

Antibodies↗
PubMed1980

[Diagnostic possibilities by penetration tests (author's transl)].

Described in this paper is a new penetration chamber in which to test the penetration capacity of spermatozoa in cervical mucus. The technique has proved to be highly suitable for penetration testing practice and is likely to provide valuable information for diagnosis.

Cervix Mucus↗
PubMed1980

[Comparative morphological and functional studies on infertility of males (author's transl)].

Male infertility is common. It usually is attributable to impaired maturation of germinal cells. The causes are related to the following factors: 1. Defective development of the gonadal ridge; 2. Abnormality of chromosomes; 3. Disorders in pituitary function; 4. Exogenous effects; 5. Unknown aetiology. -- Differentiation is difficult between those causes which often cannot be discovered at all. Therefore, assessment of male infertility is undertaken with reference to findings obtained from seminal, histological, immunological, cytogenetic, and hormonal tests as well as from electron microscopy.

Humans↗
PubMed1979

[Immunoglobulin content of the amniotic fluid and umbilical cord blood].

The content of immunoglobulins IgG, IgA and IgM and total protein in amniotic fluid and foetal serum of healthy pregnant women and of pregnant women with anti-D-antibodies has been determined by the single radial immunodiffusion and biuret method. IgG and IgA was detectable in all samples of amniotic fluid, whereas IgM was only present in 10 p.c. IgA increased in amniotic fluid to the end of pregnancy and it can indicate the maturity of fetus. The pregnant women with anti-D-antibodies have high IgG values in the amniotic fluid with the begin of 34th week of pregnancy.

Amniotic Fluid↗
PubMed1979

[Theoretical and clinical aspects of the penetration test].

An overlook is given about the theory of penetrating tests. The assay of tests depends from some biological factors concerning sperms, spermplasma and the cervix mucus itself. The penetrating tests are important in functional disorders in the area of cervixsperm. In negative results hormonal defects and immunological factors have to be considered.

Cervix Uteri↗
PubMed1979

[Results of testicular biopsy in azoospermia].

The causes of male infertility generally fall into three categories: pretesticular, testicular and posttesticular causes. The pretesticular causes are extragonadal endocrine disorders, such as those originating in the pituitary or the adrenals, which have an adverse effect on spermatogenesis. The testicular causes of infertility are conditions in which the primary defects reside in the testes. The posttesticular causes of infertility consist mainly of obstructions of the ducts leading away from the testes. Testicular biopsy is an important method in the diagnosis and management of male infertility.

Biopsy↗
PubMed1979

[Status and developmental trends of immunologic contraceptive technics].

The development of contraceptive vaccines is matter of international research. Among antigens possible unique to the reproductive process, sperm antigens, antigens of seminal plasma and of the ovum, antigens of placental hormones and of placenta specific proteins appear suitable targets of the production of fertility regulating vaccines. High specifity of these vaccines can prevent cross reaction with other body cells.

Animals↗
PubMed1978

[Spermatozoa immunity].

A report is given on the spermatozoan and seminal plasma antigens and the possibilities of immunization of woman and man with these antigens. The occurence of sperm antibodies is demonstrable with more or less specific methods. The clinical importance of sperm antibodies for female fertility depends on quality of semen and many other factors. The local immune reactions to sperm and other reproductive antigens in the female genital tract has been inferred from the detection of higher titer of activity in tissue than was found in the circulation.

Adult↗
PubMed1978

[Intra-uterine pressure measurements during early pregnancy using endoradioprobes].

The intrauterine pressure was determined by means of endoradiosonde in 42 pregnant women before induced abortion. It was measured the tonus and after stimulation with oxytocin the liminal value the basic tonus and the highest increase of pressure. The values of pressure were higher after artificial rupture of the amnion than before. Telemetering of intrauterine pressure is an advantageous method.

Abortion, Induced↗
PubMed1978

[Total protein and immunoglobulin levels in the body fluids of healthy and Rh-incompatible pregnant women].

The content of total protein and immunoglobulins in blood serum, saliva and breast-milk of healthy pregnant women and of pregnant women with anti-D-antibodies has been determined by the biuret-method and radial immunodiffusion. The values of immunoglobulins in every body fluid are typical and they respond to pregnancy. The investigations vindicate theory of local antibody synthesis. The differences of immunoglobulins from healthy pregnant women and from patients with antibodies were discussed.

Blood Group Incompatibility↗