Search PubMed⌕ Search

Biomedical subjects

H Diringer

Publications and source records attributed to H Diringer.

89 records · Page 5Linked to original sources
PubMedDate not supplied

Scrapie infectivity, fibrils and low molecular weight protein.

The development of a short incubation model of scrapie (strain 263K), in golden hamsters has added impetus to the purification of the infectious agent. Our own attempts have been based on methods pioneered by Millson and developed by Prusiner. We present here results indicating that a purification factor of up to 10(4) with respect to protein may now be possible. Fractions from brain with high infectivity had a sedimentation range of 70-300S and contained an abundance of fibrils closely similar to the scrapie-associated fibrils (SAF) discovered by Merz et al.. Material of molecular weight (Mr) 26,000, which is probably protein, appears to be a major constituent of the fibrils. The association between infectivity and fibrils raises two possibilities: the fibrils are an infectious form of the scrapie agent or they are a pathological response to scrapie infection.

Animals↗
PubMedDate not supplied

The specific labeling in hypertonic medium of a spleen protein.

The biosynthesis of 12 000 dalton protein species released into the medium by mouse spleen was resistant to the inhibitory effect of hypertonic medium on protein synthesis. This protein fraction comprised 10% or more of the total radioactive protein in the medium but could hardly be detected in the spleens. It was labeled very often to a higher extent in diseased animals. It is not related antigenically to either beta-microglobin, mouse-interferon, or to mouse immuno-globulins. A preferential uptake into cells of certain organs, which, when themselves incubated in organ cultures did not synthesize and release the protein, was observed.

Animals↗