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H Dieplinger

Publications and source records attributed to H Dieplinger.

74 records · Page 5Linked to original sources
PubMed1980

The determination of lecithin:cholesterol acyl transferase in the clinical laboratory: a modified enzymatic procedure.

A very simple and fast method for the determination of lecithin:cholesterol acyltransferase is described. The method is based on the enzymatic determination of free cholesterol in a serum or plasma sample before and after 40 min incubation at 37 degrees C. The proposed procedure is particularly useful for routine application in a clinical laboratory. It works with a coefficient of variation of less than 5%.

Cholesterol↗
PubMedDate not supplied

Lp(a) phenotyping by immunoblotting with polyclonal and monoclonal antibodies.

A new method that allows rapid phenotyping of genetic Lp(a) glycoprotein types in large numbers of samples is described. The method is based on sodium dodecyl sulfate gel electrophoresis of reduced serum or plasma in horizontal slab gels followed by immunoblotting with polyclonal anti-Lp(a) lipoprotein or monoclonal anti-Lp(a) glycoprotein antibodies. Phenotyping of 194 unrelated, healthy subjects resulted in Lp(a) allele frequencies of Lp(a)B = 0.013, Lp(a)S1 = 0.032, Lp(a)S2 = 0.106, Lp(a)S3 = 0.096, Lp(a)S4 = 0.156, and Lp(a)O = 0.600, and confirmed the recently recognized association of Lp(a) glycoprotein phenotype with Lp(a) lipoprotein concentration. The new procedure is suitable for large-scale population, genetic, and epidemiologic studies and may be important for atherosclerotic risk assessment.

Adolescent↗