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H Deguchi

Publications and source records attributed to H Deguchi.

At least 91 records · Page 5Linked to original sources
PubMed1990

Immunopathological study of neuropeptide expression in human salivary gland neoplasms.

The immunoreactivity of anti-neuron-specific enolase (NSE) and anti-Leu-7 on formalin-fixed sections of human salivary gland neoplasms was determined by the avidin-biotin-peroxidase complex method. In addition, neuropeptides, such as vasoactive intestinal polypeptide, somatostatin, and substance P, in human salivary gland neoplasms were expressed, whereas other polypeptides, including glucagon, cholecystokinin, leu-enkephalin and calcitonin, were absent. When 182 paraffin-embedded examples of human salivary gland tumors, including 112 benign and 70 malignant neoplasms, were examined immunohistochemically, positive immunoreactivity was observed in: 51 cases with NSE (59%) and 46 cases with Leu-7 (54%) of 86 pleomorphic adenomas; 11 cases with Leu-7 (61%) of 18 Warthin's tumors; 7 cases with Leu-7 (58%) of 12 acinic cell carcinomas; 5 cases with NSE (31%) of 16 adenoid cystic carcinomas; 5 cases with NSE (42%) and 4 cases with Leu-7 (33%) of 12 adenocarcinomas; 4 cases with NSE (25%) and 6 cases with Leu-7 (38%) of 16 undifferentiated carcinomas. The other tumors, such as oxyphilic adenomas, basal cell adenomas, epidermoid carcinomas, and mucoepidermoid carcinomas, were nonreactive. Neuropeptides were observed in the neoplastic epithelial cells of certain tumors such as Warthin's tumors, acinic cell carcinomas, adenocarcinomas and undifferentiated carcinomas. These findings suggest the possibility that cells of neuroendocrine origin, present in certain neoplastic salivary gland epithelia may play a significant role in the histogenesis of human salivary gland neoplasms.

Adenoma↗
PubMed1990

[Experimental investigations of the propagation mechanism and the nerve regulatory mechanism of the interdigestive migrating electric complex in intestinal movement].

Segments and Thirty-Vella type loops of the small intestine of dogs were prepared, and the excitation propagation pattern of the interdigestive migrating electric complex (IMEC) was observed by electromyography. The nerve regulatory mechanism in intestinal movement was investigated. The results indicated that first, the frequency of the basic electric rhythm (BER) is controlled so that a downward gradient is formed from the upper to the lower intestine, but this gradient continues to be controlled by a two-dimensional control mechanism involving parietal factors and extrinsic nerves due to transection of the intestines and damage to the extrinsic nerves in the dominant region. However, propagation of the IMEC in the small intestine appeared to be regulated by a combination of the two-dimensional control mechanism involving extrinsic and parietal nerves corresponding to the BER control mechanism. It was clear that the propagation mechanism of IMEC is related to the BER frequency, and propagation of the IMEC is downward in accordance with the frequency gradient of BER.

Animals↗
PubMed1990

[Immunohistochemical study of the localization and distributional patterns of CA 19-9 and CEA in primary and metastatic nodal lesions of gastrointestinal cancer].

The localized and distributional patterns of CA 19-9 and CEA have been immunohistochemically examined in 22 nodal metastasis from antigen-producing gastrointestinal cancers. CA 19-9 and CEA were found in 95% and 45% of these metastasis respectively. The same distributional and localized patterns in the metastasis as were found in the primary lesions amounted to 77% and 41% for CA 19-9, and 45% and 27% for CEA, respectively. These results suggest that the capability of producing CEA is absent or suppressed in the metastatic lesions, whereas the capability lesion but also in the metastatic lesions as well.

Antigens, Tumor-Associated, Carbohydrate↗
PubMed1990

Arrhythmias in Coxsackie B3 virus myocarditis. Continuous electrocardiography in conscious mice and histopathology of the heart with special reference to the conduction system.

In patients with acute myocarditis, arrhythmias constitute one of the crucial risk factors for morbidity and mortality. To clarify the incidences and the chronological features of arrhythmias and their correlation with the histopathology of the heart, we conducted continuous electrocardiography in an animal model of viral myocarditis, and light microscopy of the heart with special reference to the conduction system. Forty weanling C3H/He mice were divided into an inoculated group (IG) and a control group (CG), of 20 mice each. IG was injected intraperitoneally with coxsackie B3 virus and CG was injected with virus-free culture medium. Thin electrodes were implanted in their chest walls and connected with an electric impulse transmitting device so that they could move freely. ECGs were recorded continuously without anesthesia up to the 14th postinoculation day. The atrial and ventricular myocardium and the conduction system were studied by light microscopy. In CG, no arrhythmia was noted. In IG, various kinds of arrhythmias were documented. The incidences of the arrhythmia in the 20 mice were: sinus arrest 80%, second or third degree atrioventricular (AV) block 30%, premature atrial complexes 30%, premature ventricular complexes 20%, and ventricular tachycardia 10%. Arrhythmias were usually transient and recurrent, and the majority of them developed between the 6th and 13th day, when the histologic changes of the heart were greatest. There seemed to be a correlation between the kind of arrhythmias and the myocarditic lesions. Mice with sinus arrest or AV block developed histopathologic changes in the sinus node or AV conducting tissue, respectively. In the early stage of myocarditis, no inflammatory changes were apparent in the sinus node in the mice with sinus arrest.

Animals↗
PubMed1990

Experimental influenza A virus myocarditis in mice. Light and electron microscopic, virologic, and hemodynamic study.

To elucidate the heart involvement associated with influenza virus infection, the authors studied the hearts of influenza A/PR/8/34 virus-inoculated ICR mice by light and electron microscopy, cardiac catheterization, virus assay, and indirect immunofluorescence. Light microscopy showed small necrotic foci with inflammatory cell infiltration spreading in the myocardium on days 3 to 7 and evidence of healing by day 9 after inoculation. Electron microscopy demonstrated that necrotic cell debris was phagocytosed by macrophages, and that degenerating cardiocytes, macrophages, and lymphocytes were often in close contact, suggesting immunologic interactions, and that platelet thrombi were present in some capillaries on days 3 to 5. Both systolic and diastolic functions of the left ventricle (LV) were impaired on days 3 to 9 and recovered almost to normal by day 14. The virus could be isolated from the heart on days 3 to 7. Immunofluorescent preparations showed virus antigens in the vascular walls and cardiocytes until day 7. These results suggest that the acute cardiac injury was related to cytotoxic immunologic interactions, virus-induced cytolysis and, at least in part, to ischemia due to intracapillary thrombosis. Compared with coxsackie B3 myocarditis in mice, the influenza myocarditis was mild in degree and short in duration, but the influenza infection is a most common and repetitive disease in humans. The clinical implications of this animal model with myocarditis are discussed.

Animals↗
PubMed1990

Macro creatine kinase in a case of carnitine palmitoyltransferase deficiency.

A stout man was admitted to the hospital with acute rhabdomyolysis associated with macro creatine kinase (macro-CK, EC 2.7.3.2). This anomaly of CK was detected by gel electrophoresis as an atypical band between CK-MB and CK-MM, classified according to Stein's criteria (Clin Chem 1982; 28:19-24) as type 1, and identified by immunofixation electrophoresis as containing CK isoenzymes MM and MB and immunoglobulin A. Muscle biopsy showed that the etiology of rhabdomyolysis in this case was deficiency of carnitine palmitoyltransferase (CPT, EC 2.3.1.21) in the muscle. We report the first observation of macro-CK in a case of CPT deficiency; its presence may result from recurrent rhabdomyolytic attacks owing to CPT deficiency, and may suggest underlying enzymic abnormality in muscle.

Adult↗
PubMed1990

[CEA levels of draining venous blood and draining-peripheral CEA gradient in colorectal cancer patients: correlation with postoperative survival].

Correlation between preoperative CEA levels in draining venous blood (d CEA) and draining-peripheral (d-p) CEA gradient, and postoperative survival of 94 patients with colorectal cancer patients was examined. The positive rates of d CEA and d-p CEA gradient greater than 5 ng/ml (55.9% and 37.2%) in 59 alive patients were significantly (p less than 0.05) lower than those (77.1% and 57.1%) in 35 patients died of cancer recurrence within 4 years. Survival curve of the patients with positive d CEA and d-p CEA gradient were significantly (p less than 0.01) lower than those of the patients with negative d CEA and d-p CEA gradient. Survival curve of the patients with d-p CEA gradient greater than 10 ng/ml was significantly (p less than 0.001) lower than that of the gradient less than 10 ng/ml, and 4-year survival rates were 37.5% in the former patients and 68.3% in the latter patients. These results suggest that d CEA and d-p CEA gradient may be used as prognostic indicators of colorectal cancer patients. Clinically, the patients with positive d-p CEA gradient greater than 10 ng/ml are necessary to be treated as patients having very poor prognosis.

Carcinoembryonic Antigen↗
PubMed1989

Carcinoembryonic antigen levels of portal blood in gastric cancer patients.

Correlation between carcinoembryonic antigen (CEA) levels of peripheral venous and portal blood and six histopathologic and immunohistochemical variables was examined in 53 gastric cancer patients and in 8 patients with benign diseases, in order to clarify the elevation mechanism of CEA in the peripheral blood. Immunohistochemically, CEA was localized in 48 (90.6%) of the 53 cancer lesions. CEA levels of portal blood (with a mean of 136.5 ng/ml and positive rates greater than 5 ng/ml, 58.3%) were significantly higher than those (30.3 ng/ml and 22.9%) of peripheral blood in 48 patients with CEA localized cancer. However, CEA levels of portal blood were as low as 5 ng/ml and were not different from those of peripheral blood in all of the CEA nonlocalized cancer and benign diseases. Elevation of CEA in portal blood and also peripheral blood was most highly correlated with venous invasion, although CEA levels in portal blood were significantly associated with three other variables including tumor size, lymphatic invasion, and node metastasis. These variables relating to CEA elevation in the blood were significantly related to venous invasion, whereas a relationship between venous invasion and tumor differentiation and the CEA distributed pattern was not found. These results suggest that CEA may be mainly drained by the hematogenous portal system via the draining vein from CEA localized cancer cells in the invaded veins of gastric cancer lesions, and, additionally, that histopathologic CEA elevation-relating variables may secondarily affect the CEA elevation in the blood in association with the venous invasion.

Adenocarcinoma↗
PubMed1989

Cell-mediated immune cardiocyte injury in viral myocarditis of mice and patients.

The cellular immune mechanism of cardiocyte injury in viral myocarditis was investigated by observing and analyzing the interactions among cardiocytes, T cells, B cells, natural killer (NK) cells and macrophages in situ in the myocardium of our murine model (C3H/He mice) and of human patients. In murine coxsackie B3 virus myocarditis, lymphocyte subsets were identified by light and electron microscopic immunohistochemical techniques with antibodies against specific antigens of pan T (Thy 1.2), helper/inducer T (Th/i) (Lyt 1+), cytotoxic/suppressor T (Tc/s) (Lyt 2+), B (Ig+) and asialo GM1+ cells in the myocardium. In the acute phase of myocarditis, asialo GM1+ (mostly NK) cells predominated over pan T cells and peaked on day 9. Pan T cells then reached a peak on day 14. The T4/T8 (Lyt 1+/Lyt 2+) ratio was 1.3 +/- 0.5 on day 5 and reached a peak of 9.1 +/- 3.6 with an increase of Lyt 1+ cells on day 14. Thereafter, NK cells and T cells gradually decreased and could still be seen in fibrotic foci even 3 and 12 months later. B cells were so scarce that no quantitative evaluation could be made. Electron microscopy revealed that macrophages were in close contact with Th/i cells, target cardiocytes and less commonly, B cells; Tc/s and NK cells also occasionally conjugated with apparently viable or degenerated cardiocytes. Some lymphocytes were located in widened intercellular spaces of dissociated intercalated discs, and in intracytoplasmic widened confines of some cardiocytes (emperipolesis). These results suggest that in the acute phase of myocarditis, NK cells initiate the reaction, and then sensitized cytotoxic T cells and activated macrophages aggravate cell-mediated injury by their close contacts with target cardiocytes; close contacts among macrophages; Th/i cells and a few B cells, and the increased T4/T8 ratio may facilitate regulation of the complex immune network; in the chronic phase, residual but active NK and cytotoxic T cells may sustain cytotoxicity. In the endomyocardial biopsies obtained from 8 patients with viral or idiopathic myocarditis from 3 to 48 days after the clinical onset, conventional electron microscopy revealed actual contacts among cardiocytes, macrophages and lymphocytes. As in our murine model, some lymphocytes had emperipolesed in cardiocytes or were located in widened spaces of dissociated intercalated discs. In 4 of these 8 patients infiltration of Leu 2a+ Tc/s, Leu 3+ Th/i and Leu 7+ cells was identified immunohistochemically, and T4/T8 ratios varied widely from 0.1 to 3.8 in the endomyocardial biopsides.(ABSTRACT TRUNCATED AT 400 WORDS)

Adolescent↗
PubMed1988

Carcinoembryonic antigen and carbohydrate antigen 19-9 levels of peripheral and draining venous blood in colorectal cancer patients. Correlation with histopathologic and immunohistochemical variables.

Correlation between carcinoembryonic antigen (CEA) and carbohydrate antigen (CA) 19-9 levels of peripheral and draining venous blood, and 11 histopathologic and immunohistochemical variables was examined in 83 patients with colorectal cancer. CEA levels of draining blood (mean 34.5 ng/ml and positive rate greater than 5 ng/ml, 60.2%) were significantly higher than those (13.0 ng/ml and 28.9%) of peripheral blood. However, CA19-9 levels (mean 576.1 U/ml and positive rate greater than 37 U/ml, 29.5%) of draining blood were not different from those (568.0 U/ml and 29.5%) of peripheral blood. Immunohistochemically, CEA was observed in all of the 83 specimens and distributed in most of all cancer cells, whereas CA19-9 was found in 52 (62.5%) of the 83 specimens and sporadically distributed in some parts of cancer lesions in general. Elevation of CEA levels in draining and peripheral blood was most highly correlated with venous invasion, although the levels were related to four other histopathologic variables including liver metastasis, invasive layer of colorectal wall, lymphatic invasion, and Dukes' classification. Significant correlation between the CEA localized pattern of cancer cells was not found. Patients with CA19-9 nonlocalized cancer showed no elevation of the antigen levels in both peripheral and draining blood. The elevation of CA19-9 levels in peripheral blood of patients with CA19-9 localized cancer was most highly associated with lymphatic invasion, although the levels were correlated with five other variables consisting of liver metastasis, tumor differentiation, invasive layer of colorectal wall, venous invasion, and Dukes' classification out of 11 histopathologic and immunohistochemical variables. CEA levels of draining blood rose from 18.2 ng/ml and 40.3% to 30.1 ng/ml and 72.6%, respectively, after operative stimuli to cancer lesions, whereas the change of CA19-9 levels in draining blood of patients with CA19-9 localized cancer was not found during the time of operation. These results suggest that CEA may be drained mainly by the hematogenous portal system by the draining vein from the cancer cells in the invasive veins and that CA19-9 may be drained by the thoracic duct of the lymphatic system. It is also suggested that the CEA and CA19-9 elevation-relating variables may secondarily affect the CEA and CA19-9 elevation in the blood in association with the venous and lymphatic invasion of cancer lesions, respectively.

Adenocarcinoma↗
PubMed1988

[Histopathological and immunohistochemical studies on the elevation mechanism of cancer associated antigens, CEA and CA19-9, in patients with colorectal cancer].

Correlation between CEA and CA19-9 levels of peripheral and draining venous blood, and 12 histopathologic and immunohistochemical variables, was examined in 108 patients with colorectal cancer. CEA levels of draining blood (mean 62.7 ng/ml and positive rate greater than 5 ng/ml, 63.9%) were significantly higher than those (16.3 ng/ml and 31.5%) of peripheral blood. However, CA19-9 levels (mean 576 U/ml and positive rate greater than 37 U/ml, 29.5%) of draining blood were not different from those (568.0 U/ml and 29.5%) of peripheral blood. Histochemically, CEA was observed in all of the 83 specimens and distributed in almost of all cancer cells, although CA19-9 was found in 52 (62.5%) of 83 specimens and focally localized in some part of cancer lesions. Elevation of CEA levels in draining and peripheral blood was most highly correlated with grade of venous invasion and its layer of colorectal wall. While, elevation of CA19-9 levels in both draining and peripheral blood was most highly associated with grade of lymphatic invasion and node metastasis. CEA levels of peripheral blood was negative in 9 (47.4%) of 19 patients with liver metastasis. However, the levels of draining blood was negative only one (5.3%) of these patients and the mean value (273.6 ng/ml) was much greater than that (59.6 ng/ml) of peripheral blood. These results suggest that CEA may be haematogenously drained by the portal system via the draining vein from the cancer cells in the invasive veins, and that CA19-9 may be drained by the thoracic duct of lymphatic system. It is also suggested that the measurement of CEA, especially in draining blood, and CA19-9 may be available to predict the metastasis and/or recurrence of different organs such as the liver, lymph node and so on.

Antigens, Neoplasm↗
PubMed1988

[Histopathological and immunohistochemical studies on the elevation mechanism of cancer associated antigens, CEA and CA19-9, in gastric cancer patients].

Correlation between CEA and CA19-9 levels of peripheral and draining venous blood, and 11 histopathologic and 2 immunohistochemical variables, was examined in 53 gastric cancer patients. CEA levels of draining blood were significantly higher than those of peripheral blood in 48 patients with CEA producing cancer. CA19-9 levels of draining blood were not different from those of peripheral blood in 33 patients with CA19-9 producing cancer. Elevation of CEA levels in the blood was most highly correlated with venous invasion, though the levels in draining blood were related to other 10 variables except tumor location and CEA distributed patterns of cancer lesions. These 10 variables relating to CEA elevation were highly associated with venous invasion. However, tumor location and CEA distributed patterns were not associated with venous invasion. While, elevation of CA19-9 levels in the blood was most highly correlated with lymphatic invasion and node metastasis, though the levels were related to the almost same variables which were highly associated with lymphatic invasion and node metastasis as CEA elevation-relating variables. These results suggest that CEA may be hematogenously drained by the portal system via the draining vein from the CEA producing cancer cells in the invasive veins, and that CA19-9 may be drained by the thoracic duct of lymphatic system.

Antigens, Tumor-Associated, Carbohydrate↗
PubMed1987

Comparison of carcinoembryonic antigen levels between portal and peripheral blood in patients with colorectal cancer. Correlation with histopathologic variables.

Correlation between carcinoembryonic antigen (CEA) levels of peripheral and portal blood, and eight histopathologic variables, was examined in 66 patients with colorectal cancer. The change in CEA levels in the portal blood of 40 patients during operation was also examined in relation to histopathologic variables. CEA levels of portal blood (with a mean of 26.6 ng/ml and positive rate greater than 5 ng/ml, 59.1%) were significantly higher than those of peripheral blood (8.1 ng/ml, 33.3%). Elevation of CEA levels in portal and peripheral blood was most highly correlated with the venous invasion. Although the levels in the portal blood were related to six other histopathologic variables including tumor size, tumor differentiation, node metastasis, lymphatic invasion, invasive layer of the colorectal wall, and Dukes' classification except tumor location. CEA levels rose from 19.4 ng/ml and 40% to 43.6 ng/ml and 90.2% respectively following operative stimuli to cancer lesions with venous invasion. However, the levels did not rise in the lesions without the invasion. CEA levels of peripheral blood were as low as 5 ng/ml in three out of eight patients with liver metastasis. However, the levels in portal blood were much greater than 5 ng/ml in all of the patients. These results suggest that CEA may be hematogenously drained by the portal system via the draining vein from the cancer cells in the invasive veins but not by the thoracic duct of the lymphatic system.

Adenocarcinoma↗
PubMed1987

In situ analysis with monoclonal antibodies of lymphocyte subsets in myocardial biopsies from patients with dilated cardiomyopathy and idiopathic (viral) myocarditis.

This light- and electron-microscopic immunohistochemical study using monoclonal antibodies analyzes of the in situ lymphocyte subsets in endomyocardial biopsies from 11 patients with dilated cardiomyopathy (DCM) and three patients with idiopathic (viral) myocarditis (MYO). In the DCM patients both Leu 2a+ cytotoxic/suppressor T cells (Tc/s) and Leu 3+ helper/inducer T cells (Th/i) were identifiable in the myocardial lesions, and mean Th/i/Tc/s (T4/T8) ratio was 0.7 +/- 0.6 (mean +/- SD). In 9 of the 11 DCM patients, Tc/s were more numerous than Th/i cells, so the T4/T8 ratio was less than 1.0. On the other hand, the T4/T8 ratios varied widely in the three MYO patients; one of them had marked mononuclear cell infiltrates with many Th/i in the inflammatory foci and a T4/T8 ratio of 2.6. Immunoelectron microscopy revealed some Th/i in close contact with macrophages. These T cells in the myocardium of DCM and MYO patients appeared to be in vivo effector cells playing an important role in cell-mediated immune responses.

Adult↗
PubMed1986

Immunohistochemical study of the myocardium in murine Coxsackie B3 virus myocarditis using monoclonal antibodies--significance of Lyt 1 antigen-bearing lymphocytes in cell-mediated immunity.

This light- and electron-microscopic study using monoclonal antibodies provides an immunohistochemical demonstration of lymphocytes in situ in the myocardium in murine coxsackie B3 virus myocarditis. On the 7th and 9th days after virus inoculation, we observed many necrotic cardiocyte foci infiltrated with numerous inflammatory cells including macrophages and T lymphocytes. There were only a few Lyt 1-bearing lymphocytes in this phase of inoculation. On the 14th day there were many Lyt 1-bearing cells among the T cells in the foci. By immuno-electron microscopy, some Lyt 1-bearing T lymphocytes were seen in close contact with macrophages and/or other lymphocytes. These Lyt 1-bearing lymphocytes appeared to represent helper T cells, although Lyt 2.3+ lymphocytes were not examined. It is well known that activation of helper T cells leads to stimulation of B cells and cytotoxic T cells. It is assumed, therefore, that Lyt 1-bearing T lymphocytes that infiltrated into the myocardial foci play an important role in immune responsiveness at this stage of viral myocarditis.

Animals↗