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H Dancygier

Publications and source records attributed to H Dancygier.

At least 73 records · Page 4Linked to original sources
PubMed1988

Substance P modulates lymphokine activities in supernatants of cultured human duodenal biopsies.

A duodenal biopsy culture technique was used to investigate the effect of substance P on lymphokine secretion by the human gut associated lymphoid tissue. Duodenal biopsies of 7 healthy volunteers were cultured in 1 ml medium each with Pokeweed mitogen (1 microgram/ml) for 4 days at 37 degrees C. Substance P (SP) was added in concentrations ranging from 10(-12) M to 10(-6) M. Media were changed every day. Interleukin (IL)-1 beta, IL-2 and IL-2-receptor activities were determined by means of specific ELISAs. Values were referred to 5 mg biopsy weight and expressed as per cent change of basal Pokeweed mitogen-pulsed supernatant activities. 10(-8) M and 10(-6) M SP led to a decrease of IL-1 beta activity (78 +/- 13.9% and 62.8 +/- 17.1%, respectively, alpha = 0.01 each). In contrast, 10(-8) and 10(-10) M SP showed an increase in IL-2 activity up to 182.9 +/- 94.5% and 295.6 +/- 144.7%, respectively. 10(-6) M and 10(-8) M SP enhanced IL-2 receptor activities by 81.5 +/- 70% and 40.9 +/- 11.8%, respectively (alpha = 0.05). The present data demonstrate for the first time distinct SP-mediated effects on lymphokine activities in supernatants of cultured human duodenal biopsies.

Adjuvants, Immunologic↗
PubMed1988

Bombesin and its analogues inhibit interleukin-2-induced proliferation of CTLL-2 cells.

Specifically interleukin-2 (IL-2)-dependent CTLL-2 cells were incubated in short term culture in the presence of IL-2 together with bombesin and two analogues, [Lys3]bombesin and [Tyr4]bombesin in different concentrations. Cell proliferation, determined by [3H]thymidine incorporation was significantly inhibited by 35.6 +/- 5%, 39.0 +/- 5.6% and 57.0 +/- 11% (mean +/- S.E.M. of 3 independent experiments). A typically U-shaped dose-response relationship was observed, showing a maximum effect between 10(-12) and 10(-10) M. Our data support the hypothesis that this effect is mediated by a specific receptor for bombesin and closely related peptides on CTLL-2 cells. As IL-2 plays a critical role in the clonal expansion of activated lymphocytes, antagonism of the effect of IL-2 is of high biological significance.

Animals↗
PubMed1988

[Sonography of the pancreas].

Sonography was the first non-invasive method that allowed reproducible demonstration of the pancreas. The organ is located in the anterior pararenal space, it is 12-15 cm long. The pancreatic head is in direct contact with the duodenum, the body is located behind the posterial wall of the stomach and the pancreatic tail has topographic relation to the left kidney and to the hilum of the spleen. The main guiding vessel in the transverse scan is the splenic vein. The main value of sonography in acute pancreatitis is description of the course of the inflammatory process and delineation of a biliary etiology. The sensitivity in the demonstration of pseudocysts larger than 2-3 cm is about 90%. In chronic pancreatitis sonography often demonstrates a normal organ. In advanced cases an irregular echopattern with echogenic zones and irregular outer contours are seen. Endoscopic sonography is particularly valuable in demonstrating abnormalities of the pancreatic duct. Dilatation of its tributaries and irregularities of its wall are sharply depicted. 80% of pancreatic carcinomas are echopoor and carcinomas between 2-3 cm in size can be diagnosed by sonography in a few patients. However, the overall prognosis of pancreatic carcinoma has remained unchanged in the last years.

Humans↗
PubMed1988

[Primary sclerosing cholangitis].

Primary sclerosing cholangitis (PSC) is a chronic fibrous-obliterative inflammation of bile ducts of unknown origin. Genetic, infectious and immunological mechanisms are believed to be involved in the pathogenesis. The clinical signs and symptoms in the early stages are non-specific, laboratory evaluation shows a marked elevation of alkaline phosphatase and a lower rise of transaminases. The diagnosis is confirmed by endoscopic retrograde cholangiography. Histologic findings are non-specific. About 60% of patients with PSC have chronic inflammatory bowel disease, mainly ulcerative colitis. The risk of developing carcinoma of the bile ducts is also increased in patients with PSC. Therapy with glucocorticoids, colchicin, immunosuppressive agents, cholestyramin and penicillamin generally is unsatisfactory. In advanced stages liver transplantation should be performed.

Bile Ducts↗
PubMed1987

[Interleukin 2].

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Cell Line↗
PubMed1987

Substance P enhances interferon-gamma production by human peripheral blood mononuclear cells.

Substance P (SP), a neuropeptide widely distributed in the organism, has been shown to stimulate lymphocyte proliferation and immunoglobulin synthesis. However, the effect of SP on specific lymphokines is unknown. Therefore we investigated the influence of SP on mitogen-induced interferon-gamma (IFN-gamma) production in vitro. Peripheral blood mononuclear cells (PBMC) of healthy donors were isolated by density gradient centrifugation and cultured in supplemented RPMI 1640 medium with phytohemagglutinin (PHA) or pokeweed mitogen (PWM), 0.125 and 0.25 mg/liter each, and varying concentrations of SP (10(-12) to 10(-6) M). After 24 and 48 h, IFN-gamma was measured in the supernatant using radioimmunoassay. Results were expressed as percent change of controls. SP alone had no relevant IFN-gamma inducing properties. It enhanced the IFN-gamma production of PWM-stimulated cells significantly up to 18%. The maximal effect was observed at 10(-8) M. PHA-stimulated cells also increased their IFN-gamma production after addition of SP. However, due to great interindividual variations this effect did not attain statistical significance. Stimulation of IFN-gamma production by SP might be of physiological importance, since the effect was seen at concentrations comparable to those found in the body. Our data lend further support to the immunoregulatory functions of SP.

Cells, Cultured↗
PubMed1987

Computer-aided formulation of physician's reports and storage of ERCP data.

In the 2nd Medical Dept. of the Technical University of Munich, endoscopic retrograde cholangiopancreatography (ERCP) reports are written with the aid of a computer. The doctor doing the examination enters the ERCP data into screen masks. The data are stored at once in a data bank. This data bank system permits immediate error checks of the variables entered, singly and in combination. Errors can be corrected in a loop. Generating of ERCP reports is controlled by a text file, which contains both control statements and standard phrases. A special program for the document generating was developed by the authors and has been used for 15 years in various applications.

Cholangiopancreatography, Endoscopic Retrograde↗
PubMed1987

[Constipation].

Constipation is a symptom of various diseases that must be differentiated in the diagnostic work-up of the patient. Most frequently however, no structural abnormalities can be identified and constipation is part of the irritable colon syndrome. A diet deficient in bulk is the main cause of constipation in western countries. As the complaints usually last for years before a physician is consulted many patients are chronically abusing laxatives. Therefore treatment is difficult and behavioral modifications of the patient are not easily achieved. He should learn that laxatives do not "clean" the bowel and that it functions normally even if there is not "one stool a day". Although there are many laxatives that can be prescribed usefully for a short time, for long term treatment only bulk forming agents and dietary fiber supplementation are recommended.

Cathartics↗
PubMed1986

Endosonographic diagnosis of benign pancreatic and biliary lesions.

The transduodenal and transgastric route provides good access to the pancreas and to large parts of the biliary tract, and endosonography (ES) creates images of high-detail resolution. Of 87 patients examined, 33 had benign lesions of the pancreas and biliary tract. All of 12 patients with chronic calcifying pancreatitis were correctly diagnosed, and ES in addition displayed minute cysts that were missed with conventional imaging modalities. Two of three apudomas were seen with ES, and seven of eight common bile duct (CBD) stones could be identified. Adenomyosis of the papilla of Vater was missed in all of three cases. Although minor alterations of the gallbladder can be displayed by ES, benign lesions of this organ are at present not indications for ES, whereas changes in the terminal portion of the CBD are rewarding for ES examination. ES proved to be a useful morphological technique in the diagnosis of minor benign alterations of the pancreas and prepapillary stones.

Adult↗