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Biomedical subjects

H D Singh

Publications and source records attributed to H D Singh.

At least 19 recordsLinked to original sources

A novel fed-batch digestion system for biomethanation of plant biomasses.

Plant biomasses, which in the absence of adequate pretreatment pose serious operational problems in biogas production using conventional domestic flow-through digesters, can be successfully digested in a novel fedbatch digestion system that produces a steady rate of biogas. Basically, the system is a batch digestion operated with a regular input of a calculated amount of feed based on first order decay kinetics in order to maintain a regular biogas production rate. For nearly three years the system was tested in a laboratory-scale fed-batch digester (10 l) using dried water hyacinth as feed providing the desired biogas production rate. A field-scale domestic digester of masonry construction with a working volume of 10 m3 was designed and tested for about 9 months by feeding a mixture of dried water hyacinth or banana stem along with sugarcane press mud, yielding an average biogas production of 90-100% of the expected rate calculated on the basis of the feed rate.

Journal Article↗

A comparison of peak expiratory flow rates obtained with the Morgan Spirocheck and a Mini Peak Flow Meter.

To examine whether the normal Indian standards of Peak Expiratory Flow Rate (PEF) established with the peak flow meter were applicable to PEF values obtained with the Morgan Spirocheck, a comparison was made of PEF(S) measured with the Spirocheck with PEF (M) obtained with a Mini Peak Flow Meter. Data were obtained in 92 subjects (53 males between 20 and 84 years of age and 39 females between 21 and 75 years), comprising staff of the institute, patients undergoing treatment for chronic arthritis, and the accompanying relatives. There was a highly significant correlation between the two values. The values were identical in 14 subjects; the differences between the values were within 10% in 54 subjects, and in excess of 10% in only 20 subjects. In the whole group, the mean and standard error were 363.5 +/- 14.7 and 384 +/- 15.2 lit/min for PEF (S) and PEF (M) respectively. The regression equation PEF (M) = 0.961 x PEF (S) + 34.9 will enable estimation of expected value of PEF (M) corresponding to the observed value of PEF (S) when a standard obtained with a Peak Flow Meter in used assess abnormality in a patient.

Adult↗

Metabolic blocks in the degradation of beta-sitosterol by a plasmid-cured strain of Arthrobacter oxydans.

Plasmid-harbouring, sterol-decomposing organism Arthrobacter oxydans 317 was treated with sodium dodecylsulphate to obtain a plasmid-cured strain A. oxydans 317 A1 incapable of utilizing 4-androstene-3,17-dione (AD). The strain 317 A1 was unable to degrade beta-sitosterol side chain completely to form AD but could carry out partial degradation as shown by the accumulation of 3-oxochol-4-en-24-oic acid as a major metabolite and 27-norcholest-4-en-3,24-dione as a minor metabolite. The strain could form 1,4-androstadiene-3,17-dione (ADD) from 3-oxo-23,24-bisnorchol-1,4-dien-22- oic acid (BNC) to a limited extent. The existence of metabolic blocks in the conversion of 3-oxochol-4-en-24-oic acid to 3-oxo-23,24-bis-norchol-4-en-22-oic acid and further conversion to AD by the plasmid-cured strain 317 A1 was suggested. Neither the formation of ADD from AD nor the conversion of AD and ADD to 9 alpha-hydroxy derivatives leading to steroid ring opening could be done by the plasmid-cured strain but the 17 beta-reduction of AD and ADD and 1(2)-reduction of ADD were not affected by the absence of the plasmid. It was proposed that plasmid determines 1(2)-dehydrogenation and 9 alpha-hydroxylation of steroid ring structure in this organism.

Androstadienes↗

Factors limiting the microbial conversion of sterols to 17-ketosteroids in the presence of metal chelate inhibitors.

Bioconversion of sterols to 17-ketosteroids by an Arthrobacter species occurred in the presence of hydrophobic metal-chelating agents but the production of 17-ketosteroids (17-KS) was seriously limited by the rapid loss of the viability of cells in the presence of these inhibitors. Besides, the conversion was inhibited by 17-KS at concentrations of 500 ppm or more. The 17-KS formed consisted exclusively of 1,4-androstadiene-3,17-dione (ADD) and 4-androstene-3,17-dione (AD) and these were found in the extracellular medium predominantly in bound form or as molecular aggregates which may limit their accumulation. It was concluded that enhanced production of 17-KS could be achieved by protecting the viability of cells and by removing the steroid metabolites from the site of inhibition.

17-Ketosteroids↗

A study of menarcheal age in India.

A study of menarcheal age was carried out in southern India. A logit method of analysis was applied to status quo data on 1267 Tamil and Telugu speaking girls aged 9 to 18 years in 3 schools catering for different socio-economic groups. There appears to be no relationship of menarcheal age with dietary pattern classified simply as vegetarian and non-vegetarian. Differences in median age at menarche between schools correspond well with the socio-economic differences between them. The median age in the most advantaged school (12-86 years) is comparable with that in recent studies in southern and eastern Europe, and may perhaps be in advance of some recent north-west European samples.

Adolescent↗