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H D Pauer

Publications and source records attributed to H D Pauer.

At least 19 recordsLinked to original sources
PubMed1990

[The improvement of the tolerance of the kidney for ischemia with the prostacyclin analog iloprost].

Operative trauma, ischemia, release of catecholamines and thermal stimuli in consequence of harvesting, preservation and transplantation deteriorate the renal capillary circulation. By crossing of tolerance limits arises an irreversible transplant damage. The conditions of our examinations were placed in this field. By pre- and postischemic application of Iloprost was demonstrable an improvement of the ischemic tolerance of the kidney. Correction of renal hemodynamics, antiplatelet action and cytoprotection are the reasons of the protective influence of Iloprost in our opinion.

Acute Kidney Injury↗
PubMed1990

[Effect of the stable prostacyclin analog Iloprost on enzyme release during extracorporeal kidney perfusion].

The influence of the stable prostacyclin-analogue Iloprost (Fa. Schering, Berlin) in the enzyme release from extracorporalic perfused kidneys was tested in an experimental investigation. The mechanic hypothermic perfusion was carried out on four different conditions. The estimation of specially cytosolic, lysosomal, and membrane-associated enzymes in the perfusat of the kidneys showed less activities in the Iloprost-protected kidneys. The results after the replantation of the kidneys support the interpretation of the enzyme determinations.

Acid Phosphatase↗
PubMed1990

[Thirty years of hemodialysis treatment at Martin Luther University in Halle-Wittenberg].

An artificial kidney has been taken into operation in the clinic for surgery of the University of Halle-Wittenberg since 1959. This was the second clinical arrangement in the DDR. The indication of the application evolved by the acute renal failure, about the chronic renal insufficiency, within to the hemodialysis as an additive for kidney transplantation. Coworkers of our clinic were engaged vigorously in the technical development of this method in the GDR.

Germany, East↗
PubMed1987

[Effect of Iloprost (ZK 36 374) on hemodynamic parameters during kidney donor conditioning in animal experiments].

Hemodynamic parameters during the conditioning of kidney donors were tested under the influence of Iloprost (ZK 36 374), a stable prostacyclin derivate, in 12 house swine of a body weight of 20-50 kg. The electromagnetically measured blood flow in the right renal artery increased on an average of 73.2% after intraaortic infusion of Iloprost (1 micrograms/kg.min) during 15 minutes. The mean arterial blood pressure decreased simultaneously by 14.7%. The renal flow resistance (RVR), by blood pressure and blood flow calculated, decreased by 49.7% in average. Iloprost appears well-suitable for conditioning donors, it shows advantages in contrast to the hitherto used drugs.

Animals↗
PubMed1986

Improvement in the preservation of ischemically impaired renal transplants of pigs by iloprost (ZK 36 374).

Kidneys of five pigs were preoperatively perfused with iloprost in situ (infusion of 0.5 microgram X kg-1 for 15 minutes) and exposed to a warm ischemic period of 45 minutes. The behaviour of these kidneys during initial perfusion by gravity and hypothermic mechanical perfusion for 24 hours was comparable to that of kidneys having had a warm ischemic period of less than 5 minutes. After retransplantation all kidneys showed a sufficient blood flow and immediate production of urine during the test period of 3 hours.

Animals↗
PubMed1986

[Hemodynamic studies of iloprost-conditioned ischemically stressed kidneys in animal experiments].

By means of computer-assisted Doppler signal analysis on the exposed renal artery of the pig the change of the haemodynamics under the influence of Iloprost is investigated in comparison to the electromagnetic flow measurement. The Doppler sonographic establishment of the speed of the blood flow and the relative volume of the blood flow allows additional informations about characteristic changes of the form of the pulse. During the measurement in situ on the renal artery the result was during the intraarterial application of Iloprost a distinct increase of the renal blood flow with simultaneous decrease of the renal resistance, which, issuing from the Doppler signal analysis, is characterized as change of the renal resistance index. The kidneys conditioned under influence of Iloprost were retransplanted after a warm ischaemia-time of 45 minutes with following machine preservation of 24 hours and showed a stable flow characteristics for the whole investigation period. The Doppler signals directly conducted via the renal parenchyma showed a stable pulsation also in the smaller arterial vessels.

Animals↗
PubMed1986

[Effect of iloprost on aggregation behavior in kidney conditioning and changes in iloprost concentration in kidney preservation].

The conditioning of the donor by 1.0 microgram/kg X min Iloprost over 15 minutes leads to a considerable inhibition of the aggregation of thrombocytes in the domestic pig. 160% more ADP had to be added than before the application of Iloprost. When 0.5 microgram/ml Iloprost are added to the perfusion fluid and to the reperfusion fluid 0.22 microgram/ml and 0.27 microgram/ml, respectively, are to be proved in the venous efflux. Whether or not the difference is absorbed or metabolized must remain open. During the 72-hour storage of the kidney in a Euro-Collins-solution with 0.5 microgram/ml Iloprost after 24 hours a decrease of 0.34 microgram/ml, after 48 hours to 0.33 microgram/ml and after 72 hours to 0.20 microgram/ml takes place. Thus Iloprost is present in an effective concentration also after 72 hours. The decrease to one plateau rather speaks for a consumption by active metabolic effect or absorption than for a chemical decomposition.

Animals↗
PubMed1985

[Effect of the stable prostacyclin analog (iloprost) on survival of ischemically-stressed swine kidney transplants].

Kidneys of 5 pigs were pretreated in situ with Iloprost (Fa. Schering) (0.5 micrograms X kg-1 X min-1) and exposed to a warm ischaemia time of 45 minutes. The perfusion behaviour of these kidneys during the initial fall current perfusion and hypothermic machine perfusion for 24 hours was to be compared with that of kidneys with a warm ischaemia time of less than 5 minutes. After retransplantation in all kidneys a good blood supply and an immediate urine production was to be seen for the duration of the experiment of 3 hours.

Animals↗
PubMed1985

[Extended storage preservation of swine kidneys to 72 hours using iloprost].

The intraaortal infusion of Iloprost (1 micrograms/kg X min) for the duration of 15 minutes leads to an increase of the renal hematorrhea by 85.2%, to a decrease of the renal resistance by 51.6% and to a slight decrease of the average arterial blood pressure by 13.3%. After a 72-hour hypothermic storage preservation under addition of Iloprost the renal blood supply after retransplantation corresponded to the initial values of the blood flow before nephrectomy. The production of urine began after 1-2 minutes. Enzyme investigations of the transplant urine resulted in an increased excretion of the cytosolic enzymes, the structure-bound enzymes, however, remained in the area of the norm. The investigation of the electrolytes of the urine showed a hypersodiuria as well as a hypopotassiuria and calciuria. As to urea and creatinine the values were below the parameters used for comparison. In the contralateral kidneys used as control kidneys no blood supply developed after retransplantation. The application of Iloprost for the conditioning of donor kidneys is very well suited and shows some advantages compared with the preparation used up to now. The prolongation of the hypothermic storage preservation to 72 hours seems to be possible by the application of Iloprost.

Animals↗
PubMed1985

[Morphological research on DMSO-perfused kidneys].

It is reported on the morphological investigations of kidneys which had been perfused with the cryoprotective dimethylsulfoxide (DMSO). Some of these kidneys were stored for 72 hours at -4.5 degrees C and then retransplanted. The light-microscopic and electron-optical findings show considerable changes of the cells of the proximal tubulus epithelium and the collecting tubules. These in most cases focal findings of different extension do not differ on principle from the results in preservation techniques without DMSO. The dilatation of the basal labyrinth of the tubulus cell as well as the fissuration and vacuolization in the cytoplasm give references to a passive transport of substance by the cell. The results of the DMSO-perfused and at -4.5 degrees C stored kidneys show that for a beginning of the function an optimal perfusion must be supposed. On the one hand a sufficient concentration of freezing defense remedies in the renal tissue is necessary, on the other hand an optimum removal of the DMSO in the reperfusion is necessary, in order to keep the osmotic load of the kidney as low as possible.

Animals↗
PubMed1984

[Dye concentration measurements for the determination of leakage during and following normothermic cytostatic perfusion in the treatment of tumors of the extremities].

The regional normothermic cytostatic perfusion with 3000 mg Methotrexate and 1.5 mg Vincristine under rescue conditions (leucovorine) during 120 minutes was carried out in patients with malignant melanoma stage II by Sylven or other tumors of the lower extremities in the surgical university hospital Halle since 1980. Dye concentration measurements with indigo carmine for monitoring the isolation between systemic circulation and perfusion circulation were carried out during and after cytostatic perfusion. The measurements took place with a Spekol 10 of the VEB Carl Zeiss, Jena, by wave-length of 605 nm. The leakage streams of 30 perfusions amounted from 0 to 46% during the phase - regional perfusion. The dye quantity which was secreted by the kidneys during 16 hours post perfusion despite plentiful lavage of the extremity (MTX quantity) amounted from 2 to 7%.

Chemotherapy, Cancer, Regional Perfusion↗
PubMed1984

[Comparative temperature measurement by thermoprobes and infrared pyrometry in the rewarming of frozen kidneys using short wave generators].

Mini pigs' kidneys were frozen to -20 degrees C after initial hypothermic perfusion and cryopreparation with dimethyl sulphoxide. They were subsequently thawed out with short-wave radiation. During this procedure continuous temperature measurement is only possible using infra-red pyrometry without physical contact. After completion of short-wave irradiation at ca. 4 degrees C the surface and internal temperatures are measured with thermoelements. In comparison with measurement by thermoprobes, infra-red pyrometry proves to be quite practicable, with certain limitations. If a homogeneous distribution of temperature in the organ is assumed; the internal temperature could be calculated from the surface temperature. Also, continuous observation of temperature changes ist possible.

Animals↗
PubMed1979

[Ischemia, hypothermia, length of death-perfusion and temperature related factors for preservation of organs].

In order to guarantee the comparability of the results of the various working teams and experimental conditions within the preservation of organs, above all of the preservation of kidneys, it is referred to the importance which belongs to the clear establishment of the used notions, such as time of hyperthermy (duration), time of ischaemia and dead time (warm and cold). For the practice of kidney taking simple time determinations between two steps of taking have made their way. The warm dead time is the duration between clamping of the renal vessels and the beginning of the flow with cold preservation solution. This establishment is justified by the standardised conditions of taking. For reserach these pauschal temporary data are not sufficient and the exact establishment of the notion used is necessary.

Animals↗
PubMed1979

[Storage behavior of Collins' solution under various conditions].

It is reported on examinations of the precipitation reactions in the Collins solution. The solution which is produced in three and two compartments, respectively, has been given together and stored under three different conditions. During a period of 48 hours the electrolytes sodium and potassium and the pH-value were controlled. Hereby significant differences of these values are shown in the various kinds of compilation as well as between the three- and the two-compartment solution. No or only slight precipitations in the closed system are observed at 20 and 40 degrees C in contrast to the open system.

Drug Storage↗