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Biomedical subjects

H D Isenberg

Publications and source records attributed to H D Isenberg.

126 records · Page 7Linked to original sources
PubMed1969

In vitro action of carbenicillin against bacteria isolated from clinical material.

More than 500 bacteria isolated from patient material were tested against carbenicillin (disodium alpha-carboxybenzylpenicillin) by diffusion and dilution modalities. The same bacteria, which included Pseudomonas aeruginosa, Escherichia coli, Klebsiella-Aerobacter-Enterobacter group, various species of Proteus, Staphylococcus aureus and epiddermidis, enterococci, pneumococci, Streptococcus pyogenes, etc., were examined for susceptibility to other antibiotics commonly used with special emphasis on ampicillin and cephalothin. The responses of pyocine-typed P. aeruginosa were the most remarkable. The majority of these bacteria displayed susceptibility to carbenicillin by both the dilution and the diffusion techniques. The concentrations of this antibiotic used in the laboratory were of the same order of magnitude as that of the other drugs. The laboratory behavior of the other bacteria, toward this new semisynthetic penicillin derivative approximated their response to ampicillin and cephalothin.

Ampicillin↗
PubMed1969

Isolation of Salmonellae and Shigellae from an artificial mixture of fecal bacteria.

Numerous selective media, available commercially, act by suppressing "normal" bacterial inhabitants of the intestine while permitting the growth of so-called pathogenic representatives of the family Enterobacteriaceae. This investigation attempts to evaluate the action of Salmonella-Shigella (SS) agar, xylose lysine desoxycholate (XLD) agar, and hektoen enteric (HE) agar. Salmonellae and shigellae, isolated from clinical material, were mixed in various ratios with escherichiae, Klebsiella-Enterobacter-Serratia group bacteria, and members of the tribe Proteeae, also of clinical origin. Several of the mixtures were plated in multiple dilutions on the three media. Stools in preservative were also used for evaluation of the media after the addition of definite numbers of the pathogenic bacteria. Results indicate that SS agar suppresses the shigellae along with the autochthonous members of Enterobacteriaceae. XLD and HE agars readily permit the recovery of shigellae as well as salmonellae. This recovery is not obscured by the higher yield of other species obtained with these media.

Agar↗
PubMed1967

In vitro activity of doxycycline against bacteria from clinical material.

Doxycycline (alpha-6-deoxy-oxytetracycline) was tested against various bacteria of recent clinical origin with 30-mug discs. The antibiotic susceptibility of these bacteria to commonly used antimicrobial agents was also established. Those bacteria which responded with equivocal zones of inhibition about the tetracycline compounds were tested by the tube dilution technique. Staphylococci and enterococci consistently displayed greater in vitro susceptibility to doxycycline than to tetracycline or demethylchlortetracycline.

Ampicillin↗
PubMed1963

IMMUNOLOGICAL AND TOXIC DIFFERENCES BETWEEN MOUSE-VIRULENT AND MOUSE-AVIRULENT CANDIDA ALBICANS.

Isenberg, Henry D. (Long Island Jewish Hospital, New Hyde Park, N.Y.), Jona Allerhand, James I. Berkman, and Dorothy Goldberg. Immunological and toxic differences between mouse-virulent and mouse-avirulent Candida albicans. J. Bacteriol. 86:1010-1018. 1963.-The differences between mouse-avirulent and -virulent strains of Candida albicans reside at least in part in cell surface materials which can be extracted with solvents such as ethanolethyl ether and phenol. These extracts are complex haptens which behave like endotoxins in mice and rabbits. Antibodies produced against intact and extracted cells show some strain specificity in agglutination and precipitin reactions, but underline primarily the differences between the virulent and avirulent variants. The chemical constitution of the extracted complex haptens suggests that the toxic or virulent principles are polysaccharide in nature and that the avirulent strain substitutes fats and lipids for some polysaccharides on their cell surface.

Animals↗
PubMedDate not supplied

Microbiology of the canine nasal cavities.

The microbiota of both nasal cavities were investigated in 37 dogs by both aerobic and anaerobic techniques. The predominant microorganisms were composed of enterococci and staphylococci. A surprisingly high incidence (46%) of Gram-negative rods was noted from the inferior portion of the nose. Microorganisms from the superior region of the nose, as obtained with a surgical approach, differed both qualitatively and quantitatively from the respective transnasal cultures. Thus, it appears that different bacterial populations are present within various anatomic regions of the nose and a routine transnasal culture cannot accurately reflect the microbiology of the entire nasal cavity.

Animals↗