The dissociation of rat brain membranes bearing acetylcholinesterase by the non-ionic detergent triton x-100 and an examination of the product.
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Biomedical subjects
Publications and source records attributed to H D Crone.
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1. The transfer rates of three pyridinium aldoximes, a non-quaternary pyridine aldoxime and choline across the wall of sacs from the jejunum of rats were measured in vitro.2. The transfer rates observed for any one of the quaternary compounds could be inversely correlated with the transmural potential of the particular sac studied, but there was no correlation with the rates of water or glucose transfer.3. 2-hydroxyiminomethyl-N-methylpyridinium iodide (PAM) had a transfer rate seven times less than that of its non-quaternary analogue, 2-hydroxyiminomethyl pyridine.4. Neither neostigmine nor EDTA affected the transfer rate of PAM in the conditions used.5. It was concluded that the transfer of the quaternary compounds could be explained by diffusion through aqueous pores.
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1. The calcium-stimulated incorporation of [2-(14)C]ethanolamine and l-[3-(14)C]-serine into the phospholipids of homogenates of the fat bodies from larval houseflies (Musca domestica) was studied. 2. Ethanolamine and serine acted as competitive inhibitors with one another. N-Methylethanolamine was not distinguished from ethanolamine by the system. Tris buffer was also a competitor with these compounds, and a number of other amino alcohols were inhibitory, probably competitively. 3. The incorporation of [(32)P]phosphorylethanolamine into phospholipids was observed in suspensions of whole fat bodies. This incorporation was stimulated by magnesium. 4. During the incubation of the homogenates, a calcium-stimulated breakdown of phospholipids by a phospholipase A occurred. 5. These results are compared with results published for similar mammalian systems, and their possible physiological significance is discussed.