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H Coleman

Publications and source records attributed to H Coleman.

At least 19 recordsLinked to original sources
PubMed2001

Use of calretinin in the differential diagnosis of unicystic ameloblastomas.

AIMS: Calretinin, a 29-kDa calcium-binding protein is expressed widely in normal human tissues and tumours including both unicystic and solid and multicystic ameloblastomas. The histological distinction between unicystic ameloblastomas and certain non-neoplastic odontogenic cysts can be problematic. The objective of this study was to determine whether calretinin was expressed in the lining epithelium of odontogenic keratocysts, residual and dentigerous cysts and to determine whether this calcium-binding protein could be used to distinguish these cysts from the unicystic ameloblastoma. METHODS AND RESULTS: The lining epithelium in 22 cases of odontogenic keratocyst, 26 cases of residual cyst and 20 cases of dentigerous cyst were examined for the expression of calretinin. No positive epithelial staining was observed in any of these cystic lesions. In comparison, however, 81.5% of cases of unicystic ameloblastoma showed a coarse dark brown staining of the more superficial epithelial cell layers. Scattered positive stromal and epithelial cells were present; these were interpreted as mast cells. CONCLUSIONS: Calretinin appears to be a specific immunohistochemical marker for neoplastic ameloblastic epithelium and we suggest that it may be an important diagnostic aid in the differential diagnosis of cystic odontogenic lesions and ameloblastic tumours.

Ameloblastoma↗
PubMed2001

Evaluating skill acquisition in motivational interviewing: the development of an instrument to measure practice skills.

Social work education has long emphasized the need to provide competence-based education. However, little work has been done to identify, validate and evaluate competencies in specific fields such as addictions. The purpose of this study was to isolate a set of substance abuse intervention competencies using Motivational Interviewing that could be used to teach and evaluate students. The research proceeded in three stages. First, a modified Delphi approach involving social work practitioners was used to discern a set of positive and dysfunctional skills related to Motivational Interviewing. The primary researcher taught a graduate social work class aimed at teaching students theory and skills related to this model. Students demonstrated these skills with simulated clients through videotaped interviews. Finally, three observers rated the student interviews using this developed instrument. While inter-observer agreement was lower than anticipated, this study does provide early support for the development of competencies related to specific theoretical frameworks.

Competency-Based Education↗
PubMed2001

The effects of thyroid and reproductive hormones on the viability of human buccal epithelium.

Recent findings have shown that estrogen receptors were localized in stromal cells in the oral cavity. The role of physiological doses of growth promoting hormones such as thyroid stimulating hormone (TSH) and estrogen have not exclusively studied. This investigation identifies the cytology and biochemical response of buccal epithelial in the presence of estrogen and TSH at 24, 48, and 72 hr of incubation. The cells were obtained from pre, postmenopausal women and men. The cells were seeded at a density of 10,000 cells per well and aseptic techniques, morphological evaluation, biochemical analysis (MDA), and data analysis were performed following standard lab protocols. Results of this investigation revealed that: (i) there was an initial increase in total protein observed in cells treated with TSH in comparison to the control and estrogen treated cells. This trend continued for 48 hours, and no statistical differences were observed at the 72 hour phase, (ii) there was an initial decrease in MDA levels in cells treated with TSH, however, cellular damage was evident at 48 and 72 hours phases in comparison to estrogen and control groups, (iii) morphological evaluation demonstrated that there were structural changes associated with TSH treatment. These include aggregates, nuclear shriveling and lack of cellular boundaries. Estrogen treatment showed no structural alteration throughout the experiment.

Adolescent↗
PubMed2000

Calretinin expression in ameloblastomas.

AIMS: Calretinin is a 29-kDa calcium-binding protein which is expressed in the central and peripheral nervous systems as well as in many other normal and pathological human tissues. The objective of this study was to determine whether calretinin was expressed in the epithelium of ameloblastomas. METHODS AND RESULTS: Twenty-seven cases of unicystic ameloblastoma and 31 cases of solid and multicystic ameloblastoma were studied. Five-micron sections were immunohistochemically stained using either antiserum 7696 from Swant, or antiserum 18-0211 from Zymed with a standard ABC method. The results were identical with the two antisera. Positive staining was seen in 22 cases (81.5%) of unicystic ameloblastomas. This generally consisted of diffuse, intense nuclear and cytoplasmic staining of several cell layers of the more superficial cells both in the characteristic and nondescript areas of the cyst linings. In the solid and multicystic ameloblastomas, staining occurred in 29 (93. 5%) of the cases. The staining was almost always restricted to the stellate reticulum-like epithelium, was generally intense and diffuse in distribution. Areas of squamous metaplasia stained particularly intensely as did the cells surrounding micro- and macro-cysts. In both groups of lesions, most of the cases that did not stain were intensely inflamed. CONCLUSIONS: The biological significance of calretinin expression in ameloblastomas is not known and its use as a distinctive, specific immunohistochemical marker for ameloblastic tissues remains to be confirmed. However, the results of this study raise the possibility that calretinin may be an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumours.

Ameloblastoma↗
PubMed2000

Authors' reply

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Journal Article↗
PubMed1999

Cystic degeneration in fibrous dysplasia of the jaws: a case report.

A case of nonspecific cystic degeneration complicating fibrous dysplasia of the mandible is presented. This condition is extremely rare in the jaw bones. The patient had a painless swelling of the right face measuring 10 cm in diameter, and there was no history of trauma. Radiographs showed a poorly defined, "ground glass" radiopaque lesion; a central well-defined cyst was confirmed by means of computed tomography. During surgery, a large cystic cavity with surrounding, soft fibrous bone that merged with the cortex was observed. Histologic examination showed a large non-epithelial-lined cystic cavity with a surrounding fibro-osseous lesion, which was consistent with a diagnosis of fibrous dysplasia.

Child↗
PubMed1999

Long-term histologic changes in the dental pulp after posterior segmental osteotomies.

OBJECTIVE: The purpose of this study was to examine pulp tissue for 18 months after segmental osteotomy in nonhuman primates. STUDY DESIGN: In this long-term experimental study, subapical, posterior, maxillary, and mandibular osteotomies were done in 26 baboons (Papio ursinus). Baboons were killed humanely immediately after operation and at 3, 6, 12, and 18 months, when tissues were perfusion-fixed. Longitudinal step-serial sections of dental pulps were examined. RESULTS: There was a loss of the odontoblast layer as early as 3 months after surgery. Inflammatory cell infiltrate was most marked in the early postoperative stages, and the formation of osteodentin and secondary dentin was evident after 6 months. Foci of necrosis were present in the 3-month and 6-month groups but were replaced by pulp fibrosis in the 12-month and 18-month groups. All these changes were more frequent in experimental than control teeth. CONCLUSIONS: The histologic changes seen should not affect the prognosis of teeth in subapical osteotomy segments if clinicians are careful not to damage root apices and do regular, careful, clinical, and radiographic follow-up examinations. Because many pulps healed spontaneously in the study teeth, endodontic treatment should be delayed until it is clearly needed.

Animals↗
PubMed1999

Intravascular tumour in intra-oral pleomorphic adenomas: a diagnostic and therapeutic dilemma.

AIMS: Intravascular tumour has been described very rarely in pleomorphic adenomas. The aim of this study was to establish the frequency of intravascular tumour in pleomorphic adenomas arising in minor salivary glands and to determine the biological significance of this phenomenon. METHODS AND RESULTS: Representative sections of 67 widely excised pleomorphic adenomas were examined for the presence of intravascular tumour. Sixty-two cases were derived from the palate while the remaining five were from the cheeks and lips. In instances where intravascular tumour was identified, multiple serial sections were assessed and immunohistochemical stains were performed. None of these cases showed cytological evidence of malignancy. Solid cords of intravascular tumour were present in six palatal tumours (8.9%) and consisted of plasmacytoid myoepithelial cells permeating muscular walled blood vessels and capillaries both within the tumour and capsule. Immunoperoxidase staining confirmed that the intravascular cells were phenotypically identical to those of the tumour being S100- and smooth muscle actin (SMA) positive. There is some evidence that this phenomenon represents true vascular invasion although artefactual spillage cannot be excluded. CONCLUSIONS: Although the biological significance of intravascular tumour in pleomorphic adenomas of minor salivary glands remains unknown, the occurrence of metastatic disease has not been demonstrated nor have aggressive behaviour or recurrences.

Actins↗
PubMed1999

Epilepsy education in schools.

The objective of this study was to assess the effect of an epilepsy education initiative on the management of epilepsy in Southampton schools. A self-completion questionnaire survey was undertaken of staff in 19 Southampton primary schools and ten secondary schools. Changes in knowledge and management of seizures in children with epilepsy were measured following an education session and a recommended implementation of an individual seizure protocol for children with epilepsy. There were significant changes in some areas of seizure management and knowledge. Only a small number (three) of primary schools had a protocol in place. Two primary and four secondary schools were in the process of putting the protocol in place. An improvement in knowledge and seizure management has been demonstrated. Further work is needed to implement an individual seizure protocol for all children with epilepsy.

Child↗
PubMed1998

Sialadenosis: a presenting sign in bulimia.

BACKGROUND: Sialadenosis refers to noninflammatory, often recurrent, enlargement of the salivary glands, most frequently the parotids, which is almost always associated with an underlying systemic disorder. These include diabetes, alcoholism, malnutrition, anorexia nervosa, and bulimia. It is thought that the various causes of sialadenosis all result in a common pathogenetic effect in that they produce a peripheral autonomic neuropathy which is responsible for disordered metabolism and secretion, resulting in acinar enlargement. METHODS: This paper reports a case of sialadenosis as a presenting sign in bulimia and studies the histologic and electron microscopic features of this disease. RESULTS: Light microscopy showed acini which appeared to be larger than normal and which were composed of plump pyramidal cells containing prominent zymogen granules. There was less interstitial fat, and the ducts were widely dispersed. Electron microscopy showed the acinar cells to be packed with membrane-limited, dark secretory granules some of which showed moulding of their outlines. Cellular organelles and nuclei were inconspicuous. CONCLUSIONS: Management of sialadenosis depends upon identification of the underlying cause, which must then be corrected. In bulimia, the swellings may be refractory to standard treatment modalities, and parotidectomy may be considered as a last resort to improve the unacceptable aesthetics.

Adult↗
PubMed1998

An audit of paediatric epilepsy care.

Basic standards for the process of paediatric epilepsy care were identified and applied in a clinical audit; findings were presented and the audit repeated. Standards agreed related to quality of correspondence, prescribing practice, appropriateness of drug monitoring, use of neuroimaging, and quality of requests for electroencephalography (EEG). Parent satisfaction with staff courtesy, doctor communication, and clinic visits were also assessed. In the second audit prescribing practice and appropriateness of drug monitoring had improved, but quality of patient correspondence and requests for EEG were unchanged. In both periods of care many parents were dissatisfied with the quality and amount of information provided about epilepsy. Standards of care for the medical management of children with epilepsy can be agreed and used to identify achievable improvements in that care.

Appointments and Schedules↗
PubMed1997

Intra-vascular tumour in pleomorphic adenomas--a report of four cases.

AIMS: The aim of this study was to report four cases of pleomorphic adenoma which were characterized by intra-vascular tumour. METHODS AND RESULTS: The patients ranged in age from 13-43 years, one was male while three were female. The tumours were situated in the parotid gland, submandibular gland and palate (two cases). The intra-vascular tumour consisted of single, clustered and solid cords of cells within multiple muscular walled blood vessels and capillaries both in the capsule and in the tumour. Immunoperoxidase staining confirmed that the intra-vascular cells were phenotypically identical to those of the tumour. The possibility that the intra-vascular tumour represents artefactual 'spillage' has been considered, however there is some histological evidence suggesting that this phenomenon represents true vascular invasion. CONCLUSIONS: The biological significance of intra-vascular tumour in pleomorphic adenomas is unknown. Thus far there has been no correlation with either recurrence or metastases.

Adenoma, Pleomorphic↗
PubMed1997

The rapid access paediatric clinic: a way to reduce inappropriate admissions to hospital.

With documented evidence of increased numbers of paediatric admissions to a reduced number of beds, it is important that children are admitted to hospital for appropriate reasons. Some hospitals have set up rapid access or emergency consultation clinics to try to avoid unnecessary paediatric admissions. This study examined the presenting problem of 451 patients referred by general practitioners (GPs) to the paediatric emergency clinic at Southampton General Hospital over a five month period, and the outcome for the children in terms of investigation, admission or follow-up. The most common presenting problems were gastrointestinal (26.8%), respiratory (22.8%) or infectious (19.1%). Cough or "chestiness" was the single most common presenting symptom. A total of 328 investigations was performed. After the clinic visit, 35.3% of children were discharged, 18.8% were asked to return to the clinic for a follow-up visit and 19.1% were admitted to the ward. 16.4% were given a future paediatric outpatient appointment, 7.3% were referred for specialist opinion in a different speciality, and 6.7% were advised to return to the GP for follow-up. The authors consider that the emergency paediatric clinic is appropriately used by GPs referring acute and sub-acute problems and believe that local satellite clinics run along similar lines would be welcomed by GPs, health visitors and parents. Although there is little documented evidence that rapid access paediatric clinics reduce admission, the authors consider that unnecessary admission was avoided for many of the children seen in the emergency clinic. Prospective studies are needed to confirm this.

Child↗
PubMed1996

Three-dimensional computed tomography reconstruction in treatment planning for large ameloblastoma.

A case of an unusually large mandibular ameloblastoma is reported in which standard radiologic examination failed to provide adequate visualization of the tumor's limits because of overlapping structures and the presence of compound shadows. Computed tomography imaging and three-dimensional reconstruction proved invaluable in the treatment planning of this large lesion, because it allowed the surgeon to visualize and comprehend the geography of the tumor and its relationship to surrounding structures and soft tissues and to formulate a surgical plan. Use of this technique helped ensure complete excision of the tumor without excessive removal of uninvolved tissue.

Ameloblastoma↗
PubMed1996

Neuroendocrine (carcinoid) tumor of the mandible: a case report and review of the literature.

The aim of this article is to present a case of primary neuroendocrine tumor (typical carcinoid) of the mandible that occurred in a 46-year-old black woman who was seropositive for the human immunodeficiency virus. Radiologically the lesion presented as a poorly circumscribed honeycomb radiolucency that extended from tooth 21 to the ascending ramus. Histologically the tumor cells were variously arranged in small islands, trabeculae, follicles, and slitlike spaces lined by a single layer of palisaded low-columnar cells. The follicles contained an eosinophilic colloid-like substance. Immunocytochemical staining showed diffuse, intense positivity for MAK 6, pancytokeratin, S-100, and neuron-specific enolase and focal, intense, positive staining for chromogranin A. Electron microscopy showed the presence of interdigitating cell membranes, rudimentary cell attachments, and varying numbers of membrane-bound dense core granules. Special investigations failed to reveal a primary tumor, and no metastases were found. Urine and hematologic assessment did not show any evidence of functional activity. The tumor was resected, and no recurrence or spread has been seen for 2 years. Origin from foregut-derived, immature, and functionally uncommitted endocrine cells is presumed.

Carcinoid Tumor↗