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Biomedical subjects

H Chi

Publications and source records attributed to H Chi.

At least 37 records · Page 2Linked to original sources
PubMed1995

Determination of serum aluminium using an ion-pair reversed-phase high-performance liquid chromatographic-fluorimetric system with lumogallion.

An ion-pair reversed-phase high-performance liquid chromatographic method with fluorimetric detection, using lumogallion [4-chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulphonic acid] as a ligand, has been successfully applied to the determination of aluminium in human serum. The highly fluorescent aluminium-lumogallion complex (lambda ex 505 nm, lambda em 574 nm) was separated on a LiChrosorb RP-18 column with an eluent consisting of 30% acetonitrile, 70% 0.02 M potassium hydrogen phthalate and 10 microM lumogallion. The proposed system offers a simple, rapid, selective and sensitive method for the determination of aluminium in serum. The detection limit for aluminium was 0.05 microgram/l in aqueous solution and the limit of determination was 2.2 micrograms/l in serum. The recovery of the method is generally over 90%.

Aluminum↗
PubMed1995

[Effect of interferon on filtering blebs after trabeculectomy in rabbit eyes].

A study on the suppressive effect of interferon on the formation of filtering surgical scar was carried out. Trabeculectomy was performed on 6 rabbit eyes. 100,000 Units of interferon were subconjunctivally injected at the filtering bleb during the operation and on the 3rd and 7th post-operative day. The filtering blebs, filtering function and the scanning electron microscopic examinations of the surgical areas of the walls of the globes were observed. The results show that interferon can reduce the scar, improve filtering function and can be tried clinically.

Animals↗
PubMed1994

Xeroderma pigmentosum variant: DNA ploidy analysis of various skin tumors and normal-appearing skin in a patient.

BACKGROUND: The patient with xeroderma pigmentosum provides an appropriate human model for the evaluation of tumor development and progression. METHODS: We describe a patient with variant-type xeroderma pigmentosum who developed actinic keratoses, a squamous cell carcinoma, and its metastasis into a lymph node. DNA ploidy was measured and analyzed in cells of these tumors and the patient's sun-exposed as well as sun-shielded skin. RESULTS: The sun-shielded skin showed a normal diploid DNA distribution pattern, while the sun-exposed skin had an increased number of hyperdiploid cells. The actinic keratosis showed further increased hyperdiploid cells. The squamous cell carcinoma showed a large number of hyperdiploid cells and formed an aneuploid cell fraction. Histographically, the aneuploid cell fraction became more apparent and was revealed to be a major fraction in the metastatic squamous cell carcinoma. CONCLUSIONS: The changes in the DNA ploidy pattern well reflect the clinical and histologic characteristics of the respective skin conditions and likely provide the cellular basis for the sequential or stepwise carcinogenic process in the patient's xeroderma pigmentosum skin. Further studies are necessary to determine whether the present results explain the similar carcinogenic process in sun-damaged skin of the nonpredisposed general population.

Aneuploidy↗
PubMed1994

[The use of finite population sampling and lot quality assurance sampling in estimating "immunization coverage rate" in villages (towns)].

We have used Finite Population Sampling and Lot Quality Assurance Sampling (LQAS) in a field survey of "immunization coverage rate" for EPI in three villages (towns) in Lanxi Zhejiang Province. The results showed that there was no significant difference (P > 0.05) between the rates of two samplings and between the rate of sampling and population. These results indicated that both Finite population Sampling and LQAS are cheap and simple, and can represent the immunization coverage level of population.

Female↗
PubMed1993

Isolation and age-related characterization of mouse Schwann cells from dorsal root ganglion explants in type I collagen gels.

A technique for isolation of adult Schwann cells (ScC) from dorsal root ganglia (DRG) is described. Decapsulated DRG explants embedded into type I collagen gels were cultured for 3 days in serum-free medium during which ScC migrated from the explant. These explants were then grown in serum-supplemented medium to allow ScC proliferation. On day 10 the number of ScC isolated from DRG explants per mouse was about 2.5 x 10(5), and the purity was greater than 95%. This culture system provided sufficient numbers of highly purified adult ScC in a shorter culture period (2-3 times) than other methods. We used ScC from this method to determine the age-related changes in attachment, growth, and survival of ScC cultured in serum-free medium. The attachment capacity of adult ScC on type I collagen or polylysine was similar to that of newborn ScC. However, the collagen promoted growth and survival of adult ScC but not that of neonatal ScC, indicating age-related differences of ScC properties in vitro.

Age Factors↗
PubMed1993

Fibroma of tendon sheath with ulceration.

We describe a 44-year-old man with fibroma of tendon sheath. The tumor originated in the foot and developed an ulcer during its course. It was totally excised, and the defect was reconstructed by full-thickness skin graft. The patient has been free of any recurrence during the follow-up period of one year. Fibroma of tendon sheath rarely occurs in the foot, and, to our best knowledge, this condition with ulceration is the first case reported.

Adult↗
PubMed1991

NGF enhances neurite regeneration from nerve-transected terminals of young adult and aged mouse dorsal root ganglia in vitro.

Dorsal root ganglia with nerve fibers, dissected from 3-month-old young adult mice and 26-month-old aged mice, were embedded in collagen gel. In the young adult mice regenerating neurites appeared from nerve-transected terminals after 12-24 h in culture, regardless of the length of fibers, but in the aged mice, regenerating neurites were first seen after 12-48 h. Administration of nerve growth factor to the explants markedly enhanced the growth of regenerating neurites from both the young adult and aged mice.

Aging↗
PubMed1991

Interaction of prostaglandin E1 with alpha-cyclodextrin in aqueous systems: stability of the inclusion complex.

Prostavasin, an inclusion complex of prostaglandin E1 (PGE1) with alpha-cyclodextrin (alpha-CD), is used in the therapy of thrombosis. Nuclear magnetic resonance measurements have been made to study the interaction of PGE1 with alpha-CD. The observed interaction (chemical shift) and the derived dissociation constant prove that only weak interaction forces are operative and that complete dissociation occurs upon dilution.

Alprostadil↗
PubMed1991

Study on the doping control method for the detection of narcotic analgesics and beta-blockers.

The analytical method for doping control analysis has been studied. Twenty-nine drugs including eighteen narcotic analgesics, nine beta-blockers, one stimulant and one internal standard were analysed simultaneously by gas chromatograph equipped with a nitrogen-phosphorous detector and gas chromatograph combined with a mass selective detector after derivatization of the drugs.

Adrenergic beta-Antagonists↗
PubMed1991

[Separation of epimers of anisodamine (654-2) with HPLC using chiral additives in the mobile phase].

The separation of epimers of anisodamine were studied with HPLC using a reversed phase ODS C18 column. Several chiral additives were tried in the mobile phases. d-Camphorsulfonic acid and L-(+)-dibutyl tartrate showed good resolution ability for the separation of the isomers, a pair of epimers of anisodamine were well separated; while beta-cyclodextrin appeared to have no selectivity for the epimers.

Chromatography, High Pressure Liquid↗
PubMed1990

The effect of Chinese tea on the occurrence of esophageal tumors induced by N-nitrosomethylbenzylamine formed in vivo.

Five groups of rats (22 per group) were intubated with precursors of N-nitrosomethylbenzylamine (NMBzA), i.e., methylbenzylamine (1 mmol/kg body wt) and sodium nitrite (0.5 mmol/kg body wt), and with five different varieties of Chinese tea twice a week. One positive control group was intubated with precursors of NMBzA without tea, and a negative control group was given tap water and sodium nitrite only. After 12 weeks of treatment, significantly fewer lesions and papillomas in the esophageal mucosa were found in the tea-treated group than in the positive control group. The results confirmed the anticarcinogenic effects of Chinese tea in rats by blocking the in vivo formation of NMBzA.

Animals↗