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Biomedical subjects

H C Lane

Publications and source records attributed to H C Lane.

216 records · Page 12Linked to original sources
PubMed1968

Stimulation of indoleacetic Acid oxidase and inhibition of catalase in cotton extracts by plant acids.

Activity of indoleacetic acid oxidase in partially purified extracts from cotton is stimulated by small amounts of malate, succinate, fumarate, and other plant acids. The stimulation is apparently due to inhibition of catalase, which is detectable in certain preparations. The lag phase of indoleacetic acid oxidation by crude preparations is eliminated by steps in processing which conceivably either denatures or dilutes catalase, or concentrates inhibitors to catalase.

Journal Article↗
PubMedDate not supplied

Body composition in clinically stable men with HIV infection.

Clinically stable HIV-infected men (N = 106) receiving investigational antiretrovirals were recruited. Subjects were divided into three HIV disease severity groups by CD4+ cell count. Standard measures of body composition were assessed, as well as serum measures of visceral protein stores and kilocalorie intake. Group 1 subjects (CD4+ T cells < 200) had significantly lower measures of body fat as compared with Group 2 (CD4 between 200 and 600) and Group 3 (CD4 > 600) despite adequate kilocalorie intake. Group 2 and Group 3 were not significantly different from each other. Our entire cohort had significantly lower muscle mass compared to norms. Our data demonstrate that people with advanced HIV disease have reduced muscle and fat.

Adult↗
PubMedDate not supplied

Laboratory methods in the diagnosis and prognostic staging of infection with human immunodeficiency virus type 1.

Laboratory evaluation of infection with human immunodeficiency virus type 1 (HIV-1) may involve detection of antibodies to HIV-1, direct detection of HIV-1 itself, and measurement of an individual's immunologic status at the time of presentation. The ELISA is currently the preferred initial screening test, although a variety of other rapid immunoassays have also been developed. Methods defining the antigenic specificity of the antibody response, such as the western blot, have become standard confirmatory tests in this setting. CD4+ cell enumeration and the HIV-1 antigen capture assay are useful in predicting the course of HIV-1 infection and in monitoring antiretroviral therapies. Newer techniques of HIV-1 co-cultivation permit the characterization of viral isolates and the stratification of patients and facilitate monitoring of the effects of antiretroviral agents. The polymerase chain reaction is of value in identifying HIV-1 infection in individuals with inconclusive serologic results. Judicious use of other laboratory tests, including surrogate markers such as beta 2-microglobulin, also provides prognostic information potentially useful in clinical management of HIV-1-infected patients.

DNA, Viral↗
PubMedDate not supplied

Presence of intracytoplasmic IgG in the lymphocytic infiltrates of the minor salivary glands of patients with primary Sjögren's syndrome.

The nature of the minor salivary gland B cell infiltrates in 13 patients with primary Sjögren's syndrome was studied utilizing an immunoperoxidase staining technique. A group of 6 patients without primary Sjögren's syndrome undergoing lip biopsy for evaluation of dry mouth served as controls. The patients with primary Sjögren's syndrome, in contrast to the control group, were found to have a large percentage of cells containing intracytoplasmic IgG. This finding points to the minor salivary gland as a site of localization of the activated B cell in patients with primary Sjögren's syndrome.

Adult↗
PubMedDate not supplied

The generation of CD4 T lymphocytes in patients with HIV infection.

The immune systems of patients with HIV infection are characterized by a vigorous turnover of lymphocytes that represents an exaggeration of the normal homeostatic mechanisms controlling lymphocyte growth and death in an attempt to compensate for the destructive forces of the human immunodeficiency virus (HIV). Studies of naive and memory phenotypes of CD4 T lymphocytes; studies of lymphocyte survival and trafficking utilizing genetically marked lymphocytes and: studies of T cell receptor families at the molecular level have consistently led to the same conclusion, namely, that the main source of new CD4 T lymphocytes in patients with HIV infection is through the peripheral expansion of existing cells. This is true whether these expansions are due to the blockade of viral replication with anti-viral agents or through the acceleration of T cell proliferation with interleukin-2 (IL-2). Thus, once one has lost existing elements of the T cell repertoire those elements may be lost forever. These data have profound implications regarding the importance of early intervention in patients with HIV infection.

Adult↗