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Biomedical subjects

H Bucker

Publications and source records attributed to H Bucker.

41 records · Page 3Linked to original sources

Survival of bacterial spores under some simulated lunar surface conditions.

Spores of Bacillus subtilis were exposed to simulated lunar environmental factors, in order to estimate the chance of living matter to survive on the moon. Vacuum, radiation and extreme temperature were selected and their individual and combined influence was tested. High vacuum up to 2 x 10(-7) torr and ultra-high vacuum up to 5 x 10(-9) torr, ultraviolet rays (254 nm) and a temperature of 80 degrees C were used. The results were compared with those of experiments on vegetative cells.

Bacillus subtilis↗

Survival of microorganisms under simulated space conditions.

Monocellular layers of stationary phase cells of Escherichia coli B/r were exposed to high vacuum of 10(-6) Torr at different temperatures. After simultaneous irradiation with UV of 254 nm or X-rays, the survival was tested as colony forming ability. In vacuum the sensitivity to UV and X-rays was enhanced compared with the controls at atmospheric pressure. Of UV-inactivation in vacuum no photoreactivation was found.

Atmospheric Pressure↗

Discussion of a possible contamination of space with terrestrial life.

Theoretical considerations were made on what forces and energies affect a particle the size of a microorganism near the Earth and what conditions result for the particle. A transfer of terrestrial life to other celestial bodies seems impossible unless it is done by a spacecraft. This possibility cannot be excluded even after sterilization of the spacecraft on Earth, for in higher layers of the atmosphere the spacecraft could be contaminated again. In this case microorganisms would be subjected to a space flight where decompression is much slower initially than is the case on leaving the Earth's surface with the spacecraft. With these considerations, experiments on microorganisms were carried out in a vacuum chamber. For testing the effect of vacuum on the rate of desorption, mainly of water, measurement was made by a mass spectrometer. In the experiments the decompression rate was varied and different microbiological forms were used, namely, spores and vegetative cells of different cell wall structures. Further experiments in the ultrahigh-vacuum range are necessary and the problems involved are discussed.

Atmospheric Pressure↗

The biological effectiveness of HZE-particles of cosmic radiation studied in the Apollo 16 and 17 Biostack experiments.

The Biostack experiments I and II were flown on board the Apollo 16 and 17 command modules in order to obtain information on the biological damage produced by the bombardment of heavy high-energy (HZE) particles of cosmic radiation during spaceflight. Such data are required for estimating radiation hazards in manned spaceflight. Seven biological systems in resting state (Bacillus subtilis spores, Colpoda cucullus cysts, Arabidopsis thaliana seeds, and eggs of Artemia salina, Tribolium castaneum and of Carausius morosus) were accommodated in the two Biostacks. By using a special sandwich construction of visual track detectors and layers of biological objects, identification of each hit biological object was achieved and the possible biological damage correlated with the physical features of the responsible HZE-particle. In the different systems the degree of damage depended on whether the hit cell was replaceable or not. A high sensitivity to HZE-particle bombardment was observed on Artemia salina eggs; 90% of the embryos, which were induced to develop from hit eggs, died at different developmental stages. Malformations of the abdomen or the extremities of the nauplius were frequently induced. In contrast, the growth of hit Vicia faba radiculae and the germination of hit Arabidopsis thaliana seeds and hit Bacillus subtilis spores were not influenced remarkably. But there was an increase in multicaulous plants and a reduction in the outgrowth of the bacterial spores. In addition, information was obtained on the fluence of the HZE-particles, on their spectrum of charge and energy loss, and on the absorption by the Apollo spacecraft and the Biostack material itself. This will help to improve knowledge concerning radiation conditions inside of spacecrafts, necessary to secure a maximum possible protection to the astronauts.

Animals↗

The role of HZE particles in space flight: results from spaceflight and ground-based experiments.

Selected results from experiments investigating the potentially specific radiobiological importance of the cosmic HZE (= high Z, energetic) particles are discussed. Results from the Biostack space flight experiments, which were designed to meet the experimental requirements imposed by the microdosimetric nature of this radiation field, clearly indicate the existence of radiation mechanisms which become effective only at higher values of LET (linear energy transfer). Accelerator irradiation studies are reviewed which conform with this conjecture. The recently discovered production of "micro-lesions" in mammalian tissues by single HZE particles is possibly the most direct evidence. Open questions concerning the establishment of radiation standards for manned spaceflight, such as late effects, interaction with flight dynamic parameters, and weightlessness, are indicated.

Animals↗