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Biomedical subjects

H Brouwer

Publications and source records attributed to H Brouwer.

At least 19 recordsLinked to original sources
PubMed1995

[Possibilities of epidemiological studies via automated veterinary practice administration].

This study of the occurrence of tumours in dogs was carried out in order to see whether veterinary medical records can be used for epidemiological research. The relationship between tumour occurrence and breed, sex, and age was investigated by using the electronic tumourcards for 16049 dogs from three veterinary practices. Striking results include the difference in tumour occurrence between sprayed bitches (11.31%) and intact bitches (6.58%), and the high percentage of mongrels with a tumour (78.15%). Such unexpected results may be the result of selection processes underlying the composition of the population. Because these registers do not use a standard diagnostic classification system, it is difficult to identify all dogs with a specific diagnosis. This means that registers cannot be used for aetiological epidemiological research. However, they do contain valuable information for veterinary health care research and can be used as a source of cases for case-control studies, provided that a suitable mans of standardization is used.

Animals↗
PubMed1994

Rapid detection of hepatitis C virus RNA by direct capture from blood.

A new diagnostic assay for hepatitis C virus RNA detection is described. HCV genomic RNA is captured onto streptavidin-coated magnetic beads by solution hybridization with biotinylated complementary oligonucleotides. The specificity of the capture assay is confirmed using different capture oligonucleotides as well as sera representing different types of HCV. Sensitivity was determined by testing serial dilutions of a HCV infected plasma. A panel of 50 sera was tested for anti-HCV by a Line Immunoassay and for HCV-RNA by both a conventional guanidinium extraction method and the new capture assay. The specificity of the capture assay was 95.8% and the sensitivity was 92.3% compared to the standard protocol. This method provides a rapid and simple alternative for HCV-RNA detection in blood samples.

Animals↗
PubMed1994

Analysis of hepatitis C virus genotypes by a line probe assay and correlation with antibody profiles.

The 5' untranslated regions derived from 54 patients with a chronic hepatitis C virus infection were analyzed to determine the (sub)type of hepatitis C virus. Labelled polymerase chain reaction products from 5' untranslated region were used as probes for reverse hybridization in a line probe assay (Inno-LiPA) and results were validated by comparison with direct sequencing data. Five different genotypes could be distinguished based on 5' untranslated region sequence diversity. Results of typing by line probe assay and direct sequencing were similar. Antibody responses against core, NS-3, NS-4 and NS-5 epitopes were detected by RIBA-4 and Inno-LIA HCVAb II confirmatory assays. There was no consistent correlation between genotype and anti-HCV responses, although types 2, 3 and 4 hepatitis C virus isolates show poor reactivity with NS-4 ep!%"pes.

Base Sequence↗
PubMed1986

Child dental care and serial extraction: a long-term survey.

Serial Extraction is a useful means of improving the shape and function of the developing dento-alveolar system in selected cases. However, it requires an ability to recognise departures from normal development and to predict growth changes. This may be beyond the skill of the average practitioner. Much depends upon careful monitoring with the aid of study casts and radiographs. It is often necessary to carry out simple interceptive measures such as grinding of deciduous teeth or simple tooth movements at carefully selected ages. The selection of suitable cases and the procedures for managing them are illustrated by examples of treated cases.

Adolescent↗
PubMed1976

Clinical treatment with the Begg appliance.

In a series of three articles, clinical treatment with the Begg appliance has been discussed. In the March, 1973 article, the standard procedures in the three stages commonly used in the Begg method were illustrated in a series of eight cases showing the wide range of orthodontic treatment possibilities of this method. The February, 1974 article contained a case report that illustrated some of the problems connected with orthodontic observation, diagnosis, Begg treatment, and retention. In this third article, due attention has been given to common problems of the chairside worker as encountered in daily practice. Certain comments have been offered, particularly with regard to child dental care and orthodontic guidance procedures of the growing child. A treatment approach, based on an individual optimum for each patient is discussed, following the course of treatment of three cases (Figs. 1, 2, and 4) with unfavorable jaw patterns and dental problems. These are compared with others having better anatomic proportions (Figs. 3 and 5). In the last case (Fig. 5) diagnosis and treatment planning are once again reviewed. The essential decision to be made for each orthodontic treatment, namely, whether and which teeth must be removed, is discussed and illustrated. In all cases, attention has been given to the portrayal of methods and technical details through the three stages of Begg treatment.

Adult↗