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Biomedical subjects
Publications and source records attributed to H Boisvert.
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A case is reported of a bilateral atypical mycobacterial infection, occurring in a 44 year old male with antecedent silicosis. Successful surgical resection was performed after 17 months of failed chemotherapy. The atypical mycobacterium which is unclassified is intermediate between Mycobacterium avium-intracellulare. Mycobacterium terrae complex and Mycobacterium gastri. It has some similarities with the newly described species, Mycobacterium malmöense.
An M. intracellulare, rough strain (serotype 7) has been isolated from sputum of a sixty-year old patients. This patient was a political prisoner in Germany between 1942-1945 and had contracted pulmonary tuberculosis with the cavity in the upper lobe of the right lung. A strain of mycobacterium susceptible to antituberculous drugs was isolated from his sputum in 1973. Since 1979, the isolation of M. Intracellulare has been accompanied by clinical signs of pulmonary mycobacteriosis, i.e. persistence of the cavity in spite of antituberculous treatments reapparition of cough with sputum, general weakness. Intradermo-reaction with specific sensitin gives a strong positive reaction, contrasting with a weak reaction with PPD tuberculin. As the antibiogram of the strain shows a susceptibility to cotrimoxazole and erythromycin, the patient underwent a therapy with the combination sulfamethaxozale-trimethoprime and erythromycin, for 4 months. At the end of this treatment, he seems to have recovered completely. The radiological aspect of the lungs remains unchanged. Although the source of contamination remains unknown, one thinks on the basis of bibliographica data, that it can be found in human beings, or seldom in animals (a pig, a bird) or in nature (a pond).
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The action of treatment with 4 p. cent sulphuric acid for 10 minutes, was tested on 13 strains of mycobacteria. Rapidly growing mycobacteria, e.g. M. diernhoferi, M. chelonei M. abscessus, M. Fortuitum, proved much more sensitive than slowly growing mycobacteria, e.g. M. xenopi, M, scrofulacem, M. kansaii, M. avium, M. marinum, BCG, M. tuberculosis and M. Bovis. We observed the persistance of a certain number of viable bacteria in spite of a duration of contact of 120 minutes, Albumin in the culture medium used presented a protective effect together with the high concentration in bacteria.
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Wax D(P), a peptido-glycolipid found in extracts of Mycobacterium tuberculosis var. hominis, was not found in extracts of three strains of still-grown M. tuberculosis var. bovis (BCG, Marmorek and Dupray). However, extracts from three other bovine strains (Behring, LA and BB) did yield waxes D(P), and these did not differ as to their molar ratios of alanine/glutamic acid/diaminopimelic acid from human waxes D(P).
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