Search PubMed⌕ Search

Biomedical subjects

H Bohn

Publications and source records attributed to H Bohn.

At least 145 records · Page 8Linked to original sources
PubMed1978

The value of pregnancy-associated alpha2-glycoprotein in patients with colorectal cancer.

Serum pregnancy-associated alpha2-glycoprotein (alpha2PAG) levels have been measured in 67 patients with colorectal cancer. Postoperative changes in alpha2PAG concentrations were observed in patients undergoing apparently curative surgery and compared with results in patients with residual local or metastatic tumour. Significant increase in alpha2PAG levels were found only in those patients with residual or disseminated tumour. In this study alpha2PAG levels closely paralleled the clinical course of the disease in many patients.

Adult↗
PubMed1978

[Immunologic potency of pregnancy associated alpha 2-glycoprotein (alpha 2-PAG)].

The suggestion that the pregnancy-associated alpha2-glycoprotein could depress the immune response was investigated using the modified electrophoretic mobility test. The presence of the glycoprotein, at physiological levels, significantly reduced the inhibition of the migration velocity of indicator cells in the electric field caused by the MSF. In the contrary alpha2-PAG-free serum had no effect in the test system. Therefore, it can be supposed that alpha2-PAG may have an immunoregulatory function within the immunological mechanism which protects the conceptus and malignant tumor.

Cell Migration Inhibition↗
PubMed1977

[Isolation and characterization of the placental protein pp5 (author's transl)].

The isolation and characterization of placemental protein PP5 is described. The purification was achieved by use of immunoadsorbents. From the tissue of one human term placenta an average amount of 15 mg PP5 can be extracted. PP5 apparently is specific for the placenta; it could not be detected in extracts from other human tissues. In sera from pregnant women PP5 is not present or only in trace amounts (less than 0.1 mg%). In the ultracentrifuge PP5 was found to have a sedimentation coefficient of 2.8 S and a molecular weight of 36,600 daltons. Electrophoretically the protein migrates as a fast beta1-globulin. The isoelectric point was determined to be 4.6. PP5 is a glycoprotein and contains 19.8% carbohydrates (hexoses 10.0%, hexosamine 4.4%, fucose 0.4%, sialic acid 5.0%). The amino acid composition of the protein is reported, too. PP5 was found to inhibit the activity of trypsin and plasmin; the biological role of this protein therefore may be the inhibition of proteases.

Female↗
PubMed1977

[Isolation and characterization of 7S-beta1-globuline from human erythrocytes (author's transl)].

A new protein was isolated from lysates of washed human erythrocytes in a two step procedure using ionexchange chromatography and gel filtration. The protein has the electrophoretic mobility of a beta1-globulin. On ultracentrifugation the purified protein when dissolved in a 0.05 M phosphate buffer (pH 6.8), containing 0.2 M NaCl sediments with 6.88 S and shows a molecular weight of 150,000-180,000 daltons. In salt solutions with higher ionic strength the molecules dissociate reversibly into subunits which have a molecular weight of 40,000-45,000 daltons. The 7S-beta1-erythrocyte protein according to its behavior at ultracentrifugation, gel filtration and SDS poly-acrylamide gel electrophreses apparently is composed of 4 identical or similar subunits which are loosely held together by noncovalent bonds. Chemically the 7S-beta1-erythrocyte protein consists of 99% amino acids and 1% carbohydrates. The concentration of this protein in erythrocytes amounts to 250 mg per 100 ml packed red blood cells. The protein is not found in the membrane. In its physical, chemical and immunochemical properties the 7S-beta1-erythrocyte protein differs from all other well defined proteins and enzymes from human red cells thus far known.

Beta-Globulins↗
PubMed1977

Immunosuppression by human placenta lactogen (HPL) and the pregnancy-specific beta 1-glycoprotein (SP-1). Inhibition of mitogen-induced lymphocyte transformation.

The in vitro effect of human placental lactogen (HPL) and the gravidity-specific beta1-Glycoprotein (SP-1) on the lymphocyte transformation was investigated in 20 healthy women. It was shown that simultaneous incubation of the lymphocytes with HPL or SP-1 together with PHA has no influence on the incorporation rate of H3-Thymidine. When the fetal calf serum (FCS) in the lymphocyte cultures was substituted by a 3% human albumin-solution, there was a reduction of the incorporation rate of the radioactive nucleic acid precursors to 20%, as compared to the controls with FCS. After addition of HPL and SP-1 to the cultures there was a further dosis dependent decrease of the incorporation rate. When lymphocytes were preincubated with the proteins for 24 h, there was a marked reduction of the blastogenesis after additon of PHA, HPL and SP-1 under these conditions had a immunosuppressive effect on the lymphocyte transformation.

Animals↗
PubMed1977

[Isolation and characterization of pregnancy-specific beta1-Glycoprotein from urine of pregnant women (author's transl)].

With use of the immunoadsorption technique the protein antigenically related to pregnancy-specific beta1-glycoprotein (SP1), was isolated from urine of pregnant women. The physical and chemical properties of this protein were determined and compared with those of SP1. The antigen isolated from urine has a smaller molecular weight and is immunochemically only partly identical with SP1 found in pregnancy serum respectively in the placenta. The protein occuring in urine seems to be a split product of SP1 and is supposedly formed by an enzymatic degradation of the native proteins. The concentration of the protein in urine of pregnant women throughout gravidity was quantitated immunochemically using a modified Laurell-technique. During the first and second trimester of pregnancy values ranged from 0,0-0,4 mg/100 ml. In the last trimester there was an increased in the concentration of urine-SP1 with values ranging from 0,0-3,0 mg/100 ml and a mean concentration of about 1 mg/100 ml.

Female↗
PubMed1977

[Isolation and characterization of a new tissue protein (pp7) from human placentae (author's transl)].

The isolation and characterization of a new tissue protein (PP7) from human placentae is described. The placental protein PP7 is not specific for the placenta; it is found in relative high concentrations in other human tissues as well. Trace amounts of PP7 occur in erythrocytes, too. In sera this protein could not be detected with the gel diffusion test of Ouchterlony nor with the electroimmunoassay technique. PP7 has a molecular weight of around 40,000 daltons and is composed of two identical subunits which are non-covalently linked. In the ultracentrifuge PP7 sediments with 3.5 S. The isoelectric point was found to be 4.9. Electrophoretically the protein migrates in the range between alpha 2 and beta1-globulins. PP7 is a glycoprotein; its carbohydrate content amounts to 5.4% (hexoses 3.0%, Hexosamine 1.2%, Fucose 0.2%, sialic acid 1.0%). The amino acid composition of the protein is reported, too.

Erythrocytes↗
PubMed1977

Conditioning of a glass surface for the outgrowth of insect epidermis (Leucophaea maderae, Blattaria).

A method has been developed by which the surface of cover slips can be condition for rapid outgrowth of the epidermis of cockroach legs. The method permits in vitro observation of wound-healing processes. The conditioning is brought about by materials given off by the hemocytes, possibly materials from the granules of plasmatocytes which are extruded during the flattening of the cells; Coating of the glass surface with other proteins (including cockroach hemolymph serum) and carbohydrates was completely ineffective.

Blood Cells↗
PubMed1977

[Correlation of alpha-2 pregnancy associated glycoprotein with the clinical course of bronchial carcinoma (author's transl)].

Alpha-2 pregnancy associated glycoprotein is a high molecular, carbohydrate containing plasma protein with unknown biological function. During clinical treatment of 25 patients with bronchial carcinoma alpha-2 PAG was measured by the electroimmuno-diffusion method according to Laurell. A good correlation was observed between alpha-2 PAG serum levels and the course of the disease in 21 patients. Patients whose course of disease could not be influenced therapeutically showed continously increasing levels of alpha-2 PAG; patients who responded to therapy, showed a significant decrease in alpha-2 PAG serum levels. These findings indicate that periodic determinations of serum alpha-2 PAG may be helpful for controlling the course of bronchial carcinoma.

Aged↗
PubMed1977

[Serologic breast cancer follow-up monitoring using pregnancy-associated alpha-2-glycoproteins].

The variations in serum level of the alpha 2-PAG (pregnancy associated glycoprotein) were studied during the treatment of 25 patients with breast cancer. A good correlation was found between alpha 2-PAG concentration and the course of the disease. Serum alpha 2-Pag levels rose prior to the clinical recognition of metastatic disease and decreased significantly on successful treatment. alpha 2-PAG appears to have potential as an indicator of the growth of micrometastases.

Adult↗
PubMed1976

[Antifertility effect of an active immunization of monkeys with human pregnancy-specific beta 1-glycoprotein (SP1) (author's transl)].

Fertile female cynomolgus monkeys (Macaca fascicularis) were actively immunized against human pregnancy-specific beta 1-glycoprotein (SP1). Native SP1 as well as chemically modified derivatives of SP1 served as antigens. The effect of the immunization on reproduction in these animals was investigated. The immunized monkeys showed a significant reduction in fertility: the conception rate in these animals was only slightly reduced when compared with the controls; but in the immunized monkeys pregnancy often resulted in abortion. The possibility of using immunization against SP1 as a contraceptive method in man is discussed.

Abortion, Spontaneous↗
PubMed1976

Serum levels of a placental protein during gestation in the baboon.

A placental specific protein (Sp-1) isolated from human placentas was used to establish a radioimmunoassay for measurement of a baboon protein in the sera of pregnant baboons. No reaction was observed with any known pituitary or placental hormone in this assay system. Levels of Sp-1 were detected at 17 to 18 days of gestation and reached high levels by the end of the first trimester of pregnancy. A slow increase in serum levels continued thereafter until parturition. Disappearance of Sp-1 post partum was very slow and required 26 to 34 days to become undetectable in the serum. No function or role of Sp-1 in pregnancy has been suggested, but possible use of its measurement for detecting abnormal placental function deserves investigation.

Animals↗