Activation of monocytes in vitro to produce lymphocyte dysfunctions.
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Adult↗
Biomedical subjects
H Blomgren
Publications and source records attributed to H Blomgren.
Interferon and spontaneous cytotoxicity in man. III. Effect of interferon on lymphocytes and target cells in vitro.
Human leukocyte interferon (IF) was tested for its capacity to modify the spontaneous cytotoxicity of human peripheral lymphocytes for allogeneic target cells in vitro. Pretreatment of lymphocytes with IF augmented their cytotoxicity whereas pretreatment of the target cells did not increase but possibly decreased their sensitivity to the spontaneous cytotoxicity of lymphocytes.
Adult↗
Effect of prolonged in vivo administration of leukocyte interferon on the mitogen responsiveness of human lymphocytes.
Peripheral lymphocytes from patients with osteosarcoma receiving interferon (IF) as adjuvant therapy were tested in vitro for response to various mitogens. Prolonged parenteral administration of IF preparations caused no major change of the mitogen responses. The ability of IF, when added in vitro, to inhibit lymphocyte response to mitogens was not altered to any major extent by in vivo administration of IF.
Adult↗
Evidence that soluble products released by PHA-stimulated human lymphoid cells activate immunosuppressive monocytes.
Soluble mediators, lymphokines, released by stimulated lymphoid cells can modify immunological responses in several ways. In this investigation we have examined whether the supernatants of phytohaemagglutinin (PHA)-activated human lymphocytes (active SUPs) contain factors that can suppress proliferative responses of lymphocytes in vitro. The results have shown that crude preparations of peripheral lymphoid cells incubated for 24 h in active SUPs can suppress the responses of cocultured autologous lymphocytes to PPD tuberculin in vitro. Their suppressive activity was not abolished by mitomycin treatment. Some reduction of phytomitogen responses was also noted. Maximal suppressive activity was obtained within 24 h of incubation in active SUPs and it could not be induced in cell preparations depleted of monocytes-macrophages. Similar results were obtained by treating lymphoid cells with lipopolysaccharide from Escherichia coli, which is a known activator of monocytes. These results thus show that lymphokines released by stimulated lymphoid cells can activate monocytes-macrophages in such a way that they become immunosuppressive.
Cell Division↗
Characterization of lymphocytes which inhibit proliferation of human T cells exposed to lymphocyte-derived mitogenic factors.
Culture supernatants of PHA-activated human lymphocytes (active SUPs) contain factors which are strongly mitogenic for fractionated peripheral T cells. This stimulation is suppressed by certain non-T lymphocytes. It is shown that these suppressor cells can inhibit an ongoing response of T cells to active SUP and that this inhibition is reversible. Using various rosette sedimentation techniques for fractionating subpopulations of lymphocytes it is concluded that the suppressor cells lack membrane-associated receptors for C3 but possess receptors for the Fc part of IgG. This subset of lymphocytes may be an important regulator of lymphocyte proliferation during immune responses.
Adult↗
Search for specific effector functions of C3H X CBA lymphocytes which have proliferated in the spleens of irradiated CBA mice.
Injection of C3H X CBA hybrid lymphocytes into CBA mice specifically reduces the pool of host T cells which are reactive against the Mls antigen determined by the C3H genome. Since C3H X CBA lymphocytes are triggered to cell division in the spleens of irradiated CBA mice we have now examined if such activated cell populations exhibit any effector functions against CBA lymphocytes which are reactive against C3H-determined antigens. It was observed that such 'educated' cell populations were unable to significantly kill CBA lymphocytes which are triggered to cell division in C3H X CBA hosts or CBA lymphocytes which can inhibit proliferation of transplanted C3H X CBA bone marrow cells. Moreover, there was no evidence that such 'educated' cell populations can specifically kill anti-C3H-reactive CBA lymphocytes in vitro or damage CBA bone marrow cells in vivo. Thus, these results do not demonstrate that C3H X CBA lymphocytes acquire any effector functions against CBA T cells which are reactive against C3H-determined antigens.
Animals↗
Proliferation of peripheral lymphoid cells from euthyroid and hyperthyroid individuals cultured with human thyroglobulin.
Peripheral lymphoid cells from euthyroid and hyperthyroid subjects were tested for proliferative responses to human thyroglobulin in vitro using [3H] thymidine incorporation as assay. Non-fractionated lymphoid cells from both groups displayed weak stimulation. Using a rosette technique for separating sub-populations of lymphoid cells it was concluded that T-cells constituted the responding cells. The results indicate that both hyperthyroid and euthyroid subjects possess clones of T-cells which are reactive against human thyroglobulin.
Adult↗
Prognistic relevance of radiation induced immune suppression in breast carcinoma.
The extent of radiation induced immune suppression was analysed in 100 patients with carcinoma of the breast. The relative changes of lymphocyte counts and stimulations by PHA and PPD were similar in patients who differed with regard to age, size of tumour and its malignancy grade or axillary node condition. Moreover, no difference in the degree of radiation induced immune suppression existed between patients who developed recurrent disease and those who remained free of disease during a follow-up period of 4 1/2 to 7 years.
Breast Neoplasms↗
The effect of irradiation on T-cell suppression of ELISA-determined Ig production by human blood B-cells in vitro.
Human blood B-lymphocytes were co-cultured with in vitro irradiated allogeneic or autologous T-lymphocytes in the presence of pokeweed mitogen (PWM). The production of IgG, IgM and IgA, as assessed by the enzyme-linked immunosorbent assay (ELISA) was increased 2--7 times, as compared to values obtained with non-irradiated T-lymphocytes. It is suggested that the increase of Ig production is due to the selective radiosensitivity of T-lymphocytes with suppressor function.
B-Lymphocytes↗
Blood lymphocyte subpopulations in breast cancer patients following post-operative adjuvant chemotherapy or radiotherapy.
The peripheral lymphocyte population was examined in sixty-five randomized breast cancer patients before and after post-operative adjuvant cyclic chemotherapy or local radiation therapy. During 10 months of chemotherapy total lymphocyte and T cell counts were reduced to 70% and non-T cells to about 50% of pretreatment values. The population of T cells was unaltered whereas the proportion of non-T cells was significantly reduced at the end of the period. Immediately after radiotherapy total lymphocyte and T cell counts were reduced to 30%, and non-T cells to 15% of pretreatment values. During 10 months' observation total lymphocyte and T cell counts increased to 70%, and non-T cells to 50--60% of pretreatment values.
Animals↗
The effect of in vitro irradiation on PHA-mediated cytotoxicity and lymphocytes with receptors for the Fc part of Ig.
In vitro irradiation of purified human lymphocytes reduced PHA-mediated cytotoxicity (MICC) against chicken erythrocytes in a dose-dependent fashion. Lymphocytes with receptors for IgG present in both unfractionated and T-cell enriched cell preparations were depleted after X-ray exposure. T-cells with IgM-receptors, however, appeared to be more resistant. Different lymphocyte subpopulations seem to cooperate in MICC, and shifts of their proportions may be one explanation of the reduction of MICC after X-ray exposure.
Animals↗
Interferon and spontaneous cytotoxicity in man. I. Enhancement of the spontaneous cytotoxicity of peripheral lymphocytes by human leukocyte interferon.
A purified preparation of human leukocyte interferon used at this hospital in the treatment of malignant diseases was tested for its ability to modify the spontaneous cytotoxicity of peripheral lymphocytes from healthy donors. The inhibitory effect of allogeneic lymphocytes on the (3H)thymidine incorporation of a lymphoblastoid cell line, Raji, was augmented by the presence of interferon or by pretreatment of the lymphocytes with interferon. This form of pretreatment also increased lymphocytes' capacity for reducing the number of surface-adherent tumor cells in a microassay. Moreover, lymphocytes treated with interferon exhibited an enhanced cytotoxic capacity for target cells on incubation with such cells labelled with 51CR.
Cell Line↗
Cellular cytotoxicity in transitional cell carcinoma of the human urinary bladder - a summary.
The cytoxicity in vitro fo peripheral blood lymphocytes from patients with carcinoma of the urinary bladder (TCC-bladder) against allogeneic target cells from established cell lines was studied by the 51Cr-release assay. Lymphocytes from both untreated and treated TCC-bladder patients have a significantly elevated mean cytotoxicity to TCC-bladder target cells. Tumour cell destruction by lymphocytes from TCC-bladder patients shows a clear disease related specificity. In TCC-bladder patients a superimposed cytotoxicity exists, probably reflecting reactions against one or several tumour - associated antigens. In treated patients this cytotoxicity may be masked by higher incidence of cross reaction.
Carcinoma, Transitional Cell↗
Non-specific suppression of alloantibody responses of CBA mice injected with lymphoid cells from the MIs-antigen incompatible C3H X CBA hybrid.
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Animals↗
Bleomycin treatment in a case of polyarteritis nodosa. Some immunological studies.
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Aged↗
F1-hybrid anti-parental-strain reactivity. I. Impaired proliferation of CBA lymphocytes in the spleens of irradiated C3H X CBA mice pretreated with CBA spleen cells.
Previous investigations have indicated that C3H X CBA hybrid lymphocytes can neutralize lymphocytes which are reactive against the C3H-determined Mls antigen when injected into the H-2-compatible strain CBA. In this study we have inoculated C3H X CBA hybrids with CBA spleen cells and 2 weeks later lethally irradiated the mice and injected them with CBA lymphocytes to study their proliferation in the host spleen. Proliferation of lymphocytes from CBA mice, but not from C57Bl mice, was significantly impaired in such hosts, indicating that the hybrids had become immunized against the parental cells. In contrast, such an effect was not detected by treating CBA X C57Bl mice with CBA spleen cells. Humoral antibodies in the C3H X CBA hybrids capable of blocking the response of CBA lymphocytes against C3H X CBA cells could not be detected, indicating that inhibition of lymphocyte proliferation is cell mediated rather than by humoral factors.
Animals↗
F1-hybrid anti-parental-strain reactivity. II. Proliferation of C3H X CBA hybrid lymphocytes in the spleens of irradiated CBA mice.
Experiments were conducted to explore whether C3H X CBA lymphocytes can react by proliferation when they meet parental CBA spleen cells. MLC tests could not detect such a reaction, but infusion of C3H X CBA lymph node cells into irradiated CBA hosts resulted in rapid cell proliferation in the host's spleen. Such a cell proliferation was also observed after infusion of T-cell-enriched lymph node cell preparations or thymic cells from C3H X CBA donors. Upon transfer to new irradiated mice these proliferating cells continued to proliferate in CBA mice but not in C3H X CBA mice. Further evidence that the injected T-cells were the proliferating emerged from experiments where AKR X CBA lymphocytes were found to proliferate in spleens of irradiated CBA mice and that most of these cells posessed the theta antigen determined by AKR. Proliferation of F1-hybrid lymphocytes in the spleens of its irradiated parental strains was found not to be a general phenomenon and is probably restricted to some Mls-antigen-compatible strain combinations. The possibility that the C3H X CBA hybrid lymphocytes are stimulated to proliferations by CBA lymphocytes reactive against the C3H-determined Mls antigen is discussed.
Animals↗
Induction of specific unresponsiveness against the C3H M1s=antigen in mice, increase of suppressive capacity of C3H X CBA F1-lymphocytes after sensitization against CBA lymphoid cells.
Injection of adult CBA mice with lymphocytes from C3H X CBA donors leads to a specific reduction of the MLC reactivity of the recipients' lymphocytes. This effect is mediated by a population of F1-lymphocytes which do not seem to possess the relevant MLC-stimulatory M1s-antigen. In this investigation we have shown that injection of C3H X CBA mice with CBA, but not C3H spleen cells enhances the 'tolerogenic' capacity of their thymocytes and lymph node cells. A similar enhancing effect was achieved by 'educating' C3H X CBA lymphocytes in irradiated CBA, but not C3H hosts. The results indicate that C3H X CBA lymphocytes reduce the MLC-reactivity by reacting immunologically against CBA T-cells which possess recognition structures for the relevant M1s antigen. Lymphocytes of C3H X CBA mice can be sensitized against such recognition structures which thus renders them more 'tolerogenic'.
Animals↗