Search PubMed⌕ Search

Biomedical subjects

H Blomgren

Publications and source records attributed to H Blomgren.

At least 91 records · Page 5Linked to original sources
PubMed1983

Influence of human interferon-alpha therapy on cytotoxic functions of blood lymphocytes. Studies on lectin-dependent cellular cytotoxicity, antibody-dependent cellular cytotoxicity, and natural killer cell activity.

Various cytotoxic functions of blood lymphocytes were studied in 101 patients undergoing daily treatment with human interferon-alpha (IFN). Antibody-dependent cellular cytotoxicity and lectin-dependent cellular cytotoxicity were not altered to any major extent after 1 day or 1 week of IFN therapy. After 3 and 6 months of treatment a decrease in these functions was observed in most patients. Natural killer cell activity increased following the first injection of IFN and remained elevated during 1 year of IFN therapy.

Antibody-Dependent Cell Cytotoxicity↗
PubMed1983

In vitro radiosensitivity of the spontaneous cytotoxicity of blood lymphocytes in patients with untreated Hodgkin's disease.

The in vitro X-ray sensitivity of the natural killer (NK) cell activity and interferon (IFN)-inducible NK activity of blood lymphocytes obtained from 12 patients with untreated Hodgkin's disease (HD) and 12 healthy subjects was compared. It was observed that the NK activity against K562-cells decreased in a roughly linear fashion within the dose range of 4.0-16.0 Gy and there was no demonstrable difference between patients and controls. IFN treatment of the irradiated cells increased their NK activity to a higher relative extent than in unirradiated cells. On a relative basis, this increase became more pronounced as the X-ray dose was increased. The extent by which IFN could augment NK activity of lymphocytes, whether irradiated or not, did not differ between patients and controls. It is concluded that the radiosensitivity of NK activity and IFN-inducible NK activity of blood lymphocytes in HD is similar to that of healthy individuals, which is in contrast to T-cells and T-cell responses in this disease.

Adult↗
PubMed1983

Changes of blood T cell subsets following radiation therapy for breast cancer.

The changes of the size of various T cell subsets in the blood, as defined by monoclonal antibodies and Fc-receptors for IgG and IgM, were examined in 11 women after postoperative radiation therapy (45 Gy) for breast cancer. All subsets of T cells were significantly reduced at completion of irradiation. The most extensive depletion was noted in a subset with receptors for IgG, which may exert suppression. Approximately 1 year later this subset had recovered significantly in parallel with another subset, also rich in suppressor T cells, which was detected by monoclonal antibodies. On the contrary, T cell subsets rich in cells with helper activity did not exhibit any significant recovery.

Adult↗
PubMed1983

Spontaneous cytotoxicity of blood lymphocytes following local radiation therapy for carcinoma of the urinary bladder.

The spontaneous cytotoxicity of blood lymphocytes was examined before and after local radiation therapy (64 Gy) for transitional cell carcinoma of the bladder. The lymphocyte count was reduced to approximately 40 per cent at termination of treatment and remained below the pre-treatment level during a follow-up period of 18 months. The relative spontaneous cytotoxicity for 51Cr-labelled K562 cells, derived from a human myeloid leukemia, increased at completion of irradiation and remained elevated during the entire observation period. The relative cytotoxicity against Chang cells, derived from human liver, also seemed to increase but not to the same extent. The increments were probably due to an increased proportion of spontaneously cytotoxic cells bearing Fc-receptors for IgG which were significantly elevated after treatment. It was concluded that the changes differ from those occurring after local irradiation for breast carcinoma. The proportion of lymphoid tissue in relation to the blood volume which is included in the irradiated field may determine the changes of the blood lymphocyte population.

Aged↗
PubMed1983

The effect of radiation on T cell modulation of Ig synthesis by human B cells in vitro.

PWM-induced in vitro Ig synthesis was measured in cell cultures containing B lymphocytes from healthy controls and T lymphocytes collected from breast cancer patients before and after radiotherapy, respectively. The amounts of IgG and IgM did not differ between these two types of cultures. Lymphoid cell suspensions containing 3-5% monocytes were irradiated with 13 Gy and co-cultured with non-irradiated B lymphocytes. The PWM induced production of IgG and IgM in these cultures was decreased as compared to non-irradiated controls.

B-Lymphocytes↗
PubMed1983

Distribution of lymphocyte subsets following radiation therapy directed to different body regions.

Local radiation therapy may severely deplete the recirculating pool of lymphocytes as manifested by a reduction of blood lymphocyte counts. There is considerable controversy, however, concerning the changes of the distributions of subsets of blood lymphocytes during the acute and late phases after irradiation. This report provides evidence that radiation therapy directed to different anatomic sites may change the distribution of subsets of blood lymphocytes in different ways. For instance, during the acute phase after radiation therapy for breast cancer there is a more extensive reduction of the proportion of lymphocytes with receptors for C3 than that of lymphocytes with receptors for sheep erythrocytes (characteristics of B- and T-cells respectively). Such a difference, however, was not observed in groups of patients receiving pelvic irradiation. The controversy in the literature concerning the distribution of lymphocyte subsets after irradiation may thus in part be due to the fact that the patients examined have received radiation therapy to different body regions.

Adult↗
PubMed1983

In vitro and in vivo effects of interferon on the response of human lymphocytes to mitogens.

Previous studies have shown that addition of IFN to the assay in vitro inhibits the proliferative response of lymphocytes to mitogens, whereas long term treatment by IFN in vivo has no major effect on the mitogen responsiveness of tumour patient's lymphocytes. Possible reasons for the discrepancy between the results obtained following treatment by IFN in vitro and in vivo were investigated. It was observed that the proliferative response of tumour patients lymphocytes to various mitogens was not affected to any major extent 24 hr after a single injection of 3 million units of interferon-alpha (IFN-alpha). Lymphocytes from tumour patients and healthy donors were found not to differ in their susceptibility to IFNs' anti-proliferative effect in vitro. Pure IFN-beta, present in the assay throughout the incubation period, inhibited the response of lymphocytes to polyclonal mitogens and PPD showing IFN and not contaminants in the preparations to be responsible for this effect. Although the presence of IFN in the assay throughout the incubation period inhibited the proliferative response of lymphocytes, pre-treatment of these cells with IFN-alpha in vitro was found to have no major effect on their response to mitogens. We conclude that the lack of effect on the proliferative response of lymphocytes following treatment by IFN in vivo, is probably due to the fact that the lymphocytes were only treated with IFN prior to the assay.

Cells, Cultured↗
PubMed1982

Interferon and natural killer activity in multiple myeloma. Lack of correlation between interferon-induced enhancement of natural killer activity and clinical response to human interferon-alpha.

Natural killer (NK) activity of peripheral lymphocytes was measured in 39 patients with multiple myeloma prior to and during interferon (IFN) therapy. NK activity increased in a majority of patients following the first injection of IFN and remained at an increased level during 1 year of therapy. Lower doses of IFN seemed to induce a greater increase in NK activity than higher doses. No correlations could be observed between the response of the tumors to IFN therapy and pretreatment levels of NK activity, IFN-induced enhancement of NK activity in vitro or IFN-induced enhancement of NK activity in vivo.

Adult↗
PubMed1982

Adjuvant interferon treatment of human osteosarcoma.

This paper updates the results of the clinical trial to examine the efficacy of exogenous leukocyte interferon therapy as adjuvant treatment for osteosarcoma. So far the incidence of metastases is lower and the survival rate is better for the interferon-treated group than for the concurrent control group. The number of treated patients is too small at present to allow proper statistical calculations to be made.

Clinical Trials as Topic↗
PubMed1982

Changes of the spontaneous cytotoxicity of the blood lymphocyte population following local radiation therapy for breast cancer.

The capacity of the blood lymphocyte population to spontaneously lyse K562 and Chang cells In vitro was examined in women who received local radiation therapy (45.0 Gy) for breast cancer. It was observed that cytotoxicity, on a cell-for-cell basis, was significantly reduced against K562 cells at completion of irradiation. This was followed by a recovery to the pretreatment level within 3-4 months and remained relatively constant for approximately 2 yr. The pattern of these changes were reasonably similar to that of the frequency of lymphocytes expressing Fc-receptors for IgG. In contrast, the relative cytotoxicity against Chang cells was unchanged at completion of irradiation. At 3-4 months, however, the relative cytotoxicity was increased above the pretreatment level and remained elevated for at least 2 yr. The results indicate that different effector cells are involved in the spontaneous destruction of K562 and Chang cells.

Adult↗
PubMed1982

T lymphocyte subpopulations in blood following radiation therapy for breast cancer.

T helper and T suppressor lymphocyte subpopulations were examined before and after postoperative adjuvant radiation therapy for breast cancer. Absolute numbers of all T cell subsets were reduced by 70-80%. The proportion of IgG-Fc receptor-bearing T cells (Tg cells) was significantly reduced and that of IgM-Fc receptor-bearing T cells (Tm cells) significantly increased at completion of local radiation therapy with 45 Gy (4500 rad). Proportions of T suppressor (Ts) and T helper (Th) cells determined by monoclonal antibodies were not changed by radiation therapy. The overlapping between Tg and Ts subpopulations was 20-30% as examined by double labelling.

Adult↗
PubMed1982

Effect of radiation therapy and in vitro x-ray exposure on lymphocyte subpopulations and their functions.

Radiation treatment of breast cancer patients (45.0 Gy) profoundly affected the peripheral blood lymphocytes. The number of these cells was markedly reduced with non-T-cells being more extensively depleted than T-cells immediately after radiation. The long-lasting lymphopenia, on the other hand, was mainly due to reduced number of T-cells. Antigen and mitogen stimulability, MLC reactivity, pokeweed (PWM)-induced immunoglobulin (Ig) production in vitro, and different cytotoxic functions decreased. Depletion of lymphocytes largely restored the radiation-depressed lymphocyte reactivity. The effects of in vitro exposure of blood lymphocytes to x-rays were similar to those seen after radiotherapy. Non-T-cells and T-cells with Fc-receptors for IgG were relatively radiosensitive. This latter observation agreed well with demonstrated increase of PWM-induced Ig synthesis after in vitro exposure to x-rays. T-suppressor cells defined by monoclonal antibodies were, however, radioresistant. The cytotoxic functions were reduced. No correlations were found between the pretreatment immunological status or the extent of radiation-induced immunological suppression, respectively, and prognosis.

Adult↗
PubMed1982

Effect of irradiation on Concanavalin A-induced suppression of mitogen responses in human lymphocyte cultures.

Concanavalin a (Con A)-induced and Con A-inducible human suppressor lymphocytes were examined for their radiosensitivity (16 Gy). It was observed that X-ray exposure of Con A-induced suppressor cells abolished some of the suppressive activity. Exposure of lymphocyte preparations did not prevent the subsequent induction by Con A of suppressor cells capable of inhibiting mitogen responses of lymphocytes. The relative size of the Con A-inducible suppressor population in the blood was not changed in breast cancer patients after local radiation therapy.

Adult↗
PubMed1982

Influence of adjuvant chemotherapy on the blood lymphocyte population in operable breast carcinoma. Comparison between two types of treatments.

The influence on the blood lymphocyte population of two types of postoperative adjuvant chemotherapy regimes given to patients with large breast tumors or involved axillary lymph nodes have been examined. Cyclic treatment with a combination of chlorambucil, methotrexate and 5-fluorouracil was more myelotoxic and required more extensive dose reductions than treatment with cyclophosphamide, methotrexate and 5-fluorouracil with the dosage used. Both treatments reduced the size of the blood lymphocyte population and changed its cellular composition, as defined by rosette tests, to approximately the same extent. Response of the lymphocytes to specific and non-specific mitogens were also affected to approximately the same extent. A comparison of the clinical value between the two treatments has not yet been performed.

Adult↗