Biomedical subjects

H Blobel

Publications and source records attributed to H Blobel.

At least 127 records · Page 7Linked to original sources

PubMed1975

[Influence of "clumping factor" from Staphylococcus aureus on phagocytosis (author's transl)].

In phagocytosis experiments the pathogenic significance of the "clumping factor" (CF) of Staphylococcus aureus was studied. CF-positive staphylococci were engulfed considerably less by leukocytes from rabbits and cattle than CF-negative staphylococci. Equally, the engulfment of staphylococcal skeletons (SSk) coupled with partially purified CF was significantly less than that of the SSk. The SSk had been prepared by extraction of staphylocci with formic acid. By this method the SSk were freed of all substances associated with staphyloccal virulence. Before coupling with CF the SSk were activated by cyanogen bromide. These in vitro-observations could be confirmed by the corresponding phagocytosis studies in experimental body cavities of rabbits. Thus, CF apparently interfered with the engulfment of the staphylococci by the leukocytes.

Agglutinins↗

PubMed1975

[Cytotoxic effects of a leukocidin from Staphylococcus aureus (author's transl)].

In an attempt to improve the assay of leukocidin from Staphylococcus aureus a combined vital fluorochrome staining procedure with acridine organe and ethidium bromide was developed. It proved to be more suitable for the demonstration of leukocidic effects on leukocytes than phasecontrastmicroscopy. Damaged leukocytes fluoresced distinctly red and undamaged leukocytes green. With the fluorochrome-method leukocidic changes became also evident in some lymphocytes. These were not clearly demonstrated by phasecontrastmicroscopy.

Acridines↗

PubMed1975

[Effects of an antiserum prepared against purified protease of pseudomonas aeruginosa (authors transl)].

Purified protease was obtained from Pseudomonas aeruginosa. It stimulated in rabbits the production of antibodies, which could be demonstrated mainly in an indirect hemagglutination test. Injected intracutaneously into rabbits, the protease caused an increased permeability in the capillaries and in higher doses bleedings. Immunization with the protease reduced the development of these lesions. Similar findings were obtained in experiments with mice. An antiserum prepared in rabbits against purified protease did not protect mice against infection with P. aeruginosa. However, a small protective effect could be observed after active immunization.

Animals↗

PubMed1975

[Toxicity of culture supernatants from Pseudomonas aeruginosa (author's transl)].

In an effort to learn more about the pathogenicity of Pseudomonas aeruginosa we studied the culture supernatants of 20 strains of this bacterial species. Of these 5 proved to be highly toxic, 9 moderately toxic and 6 nontoxic for mice. The toxic component could not be separated from protease (elastase), neither by gel-filtration on Sephadex G-100 superfine, nor by isoelectric focusing. From this we assumed that the toxicity of the culture supernatants was associated with elastase. Purified elastase from P. aeruginosa, strain 75, elicited cytotoxic effects in cultures of L-, HeLa- and testicular-cells from calves. It apparently did not damage cultures of kidney-cells from rabbits and pigs.

Cells, Cultured↗

PubMed1974