Search PubMed⌕ Search

Biomedical subjects

H Bessler

Publications and source records attributed to H Bessler.

136 records · Page 8Linked to original sources
PubMedDate not supplied

Beta-endorphin modulation of IL-1-induced IL-2 production.

We have studied the effects of natural opioids on interleukin-1 (IL-1) -induced interleukin 2 (IL-2) production by the lymphoid cell line EL-4. beta-Endorphin (beta-end) significantly enhanced IL-2 production by IL-1-stimulated EL-4 cells. Similar results were obtained using the LBRM33-1A5 cell line. beta-End induced significant enhancement (35-100%) of IL-1-induced IL-2 production at all concentrations of IL-1 tested (2-0.25 U/ml) and the effects were seen with both IL-1 alpha and IL-1 beta. The dose response of beta-end augmentation of IL-1-induced IL-2 production was bimodal, with peak activities seen at high (10(-8)-10(-10) M) and low (10(-16) M) beta-end concentrations. The specificity of beta-end effect was studied using the opioid antagonist naloxone. Naloxone completely abolished the enhancing effects of beta-end, indicating that the effects might be mediated through binding to opioid receptors. In addition, other opioid peptides, including gamma-endorphin and enkephalins, elicited similar effects. Northern blotting analysis revealed higher levels of IL-2 mRNA in beta-end-treated IL-1-induced EL-4 cells than in IL-1-induced control cells. Thus, beta-end might enhance IL-2 production by either augmenting the transcription rate or increasing IL-2 mRNA stability. These results suggest that beta-end might play an important role in the regulation of lymphokine production in the periphery in addition to its known interactions with IL-1 in the central nervous system.

Animals↗
PubMedDate not supplied

Cytokine production in panic disorder patients.

Based on findings that stress and anxiety may modulate immune function, we compared the production of interleukin-2 (IL-2) and interleukin-3 (IL-3) by peripheral blood mononuclear cells between 24 patients with nonmajor depressed panic disorders, 9 with agoraphobia and 15 without, and 19 healthy volunteers. No differences in the production of these cytokines was noted between the patients with panic disorders and the volunteers or between the patients with and without agoraphobia. However, in the patients, a negative correlation was found for interleukin-3 production with severity of state anxiety, but not with trait anxiety or depression. This finding indicates that interleukin-3 levels may be sensitive to the presence of anxiety and stress.

Adult↗
PubMedDate not supplied

The metabolism of adenosine and distribution of adenosine receptor lymphocytes in two human circulating T cell subsets.

Human circulating E rosette forming cells (ERFC), rerosetted with sheep erythrocytes in the presence of adenosine, yielded two T-lymphocyte subpopulations: a major fraction forming E-rosettes (E resistant = ER) and a minor non-rosetting fraction (E sensitive - ES). Both T cell subpopulations converted adenosine mainly into inosine. However, ES cells metabolized adenosine more extensively than ER cells. Adenosine deaminase (ADA) activity was significantly higher in ES cells. Purine nucleoside phosphorylase (PNP) activity, as well as hypoxanthine guanosine phosphoribosyl transferase (HGPRT) activity were similar in both T cell subsets. The ratio of ADA/PNP in ES cells relative to ER cells was 1.8 suggesting that ES cells are at an earlier stage of differentiation. Enrichment of lymphocytes bearing a receptor for adenosine was demonstrated in ES cells.

Adenosine↗
PubMedDate not supplied

Functional and ultrastructural studies of Sezary cells.

A patient having clinical findings and typical ultrastructure of the peripheral white blood cells compatible with the diagnosis of Sézary syndrome is described. Studies of the synthesizing capacity of the Sézary cells showed a statistically significant increase in their capacity for protein and RNA synthesis, but no difference with regard to DNA synthesis as compared to normal lymphocytes.

Blood Proteins↗
PubMedDate not supplied

Clinical and ultrastructural observations in primary acquired sideroblastic anemia.

The clinical and laboratory findings in three patients with primary acquired sideroblastic anemia (PASA) are described. The results demonstrate that what seems to be a well defined entity of the large group of sideroblastic anemias, is in itself a heterogeneous subgroup, with differences capable of detection by more extensive studies. Electron microscopic examination of erythroid cell precursors confirmed previously described features such as deposition of large amounts of iron, mainly in the mitochondria. The precipitated serum proteins revealed an increased content of ferritin molecules only in the patients and not in control individuals, suggesting an increased supply of iron to the erythroid precursors. This finding could serve as an additional mechanism for the accumulation of iron in the erythroid precursors, considering also the defect in heme synthesis and increased permeability of the erythroid membrane for iron, described in this condition.

Aged↗
PubMedDate not supplied

Ultrastructural observations on the cells of a patient with Fanconi's anemia transforming to acute leukemia.

The ultrastructural findings in the bone marrow and peripheral blood cells of a patient with Fanconi's anemia transforming to acute myelo-monocytic leukemia are presented. They consisted of an unusual large number of nuclear pockets, bridges and appendices observed in the nuclei of the polymorphonuclear cells, monocytes and to a lesser extent in the mature normoblasts. Since the mother and two younger brothers of the propositus were found to have macrocytic anemia and pancytopenia, most probably Fanconi's anemia, their peripheral blood cells were also examined with the electron microscope. The cells of all of them showed similar nuclear alterations, although in lesser quantity. The possibility that these findings are consistent with the diagnosis of Fanconi's anemia, or they herald an evolution of the disease to acute leukemia is considered.

Adult↗