[Influence of temperature on the behavior of b-wave in the electroretinogram of dark adapted frogs exposed to serial flash stimulation].
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Biomedical subjects
Publications and source records attributed to H Berger.
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The introduction of rII mutations into polynucleotide ligase amber mutants (gene 30) of T4D bacteriophage results in the restoration of phage growth in the nonpermissive host E. coli B. When cells are mixedly infected with rII(+) -ligase(-) and rII-ligase(-) phage, growth is reduced, indicating that the rII mutations are recessive to the rII(+) alleles. Infection with the rII-ligase(-) double mutants results in nearly complete restoration of phage DNA synthesis and prevents the extensive degradation of parental phage DNA observed after infection with ligase(-) single mutants. Similar results are observed when cells are infected with ligase(-) mutants and chloramphenicol is added five minutes post infection.
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Recombination experiments were performed to assess the affect of amber mutations in 12 genes of T4D bacteriophage on genetic recombination. Crosses were performed in various suppressor-containing bacterial hosts to permit the production of progeny phage. Amber mutations in genes 32, 46, and 47 caused decreased recombination, amber mutations in genes 30, 41, 42, 43, 56, 61, and 62 caused increased recombination, whereas mutations in genes 63 and 37 showed no demonstrable effect on recombination.
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