The lactose sulfite (LS) medium recommended for the detection and identification of Clostridium perfringens in foods was compared with a reference method using tryptose-sulfite-cycloserine (TSC) agar for the enumeration of this organism in a variety of foods and food ingredients. C. perfringens was detected and enumerated in 17 of the 54 samples examined with LS broth, but its presence could be confirmed in only 9 of the samples with TSC agar. In only 2 instances, C. perfringens was detected on TSC agar but not in LS broth. A positive response (FeS + and gas +) in LS broth incubated at 46 degrees C always corresponded to the presence of C. perfringens; whereas the black colonies formed on TSC agar incubated at 37 degrees C were frequently found to be Clostridium species other than C. perfringens. Thus, because of its highly selective nature, LS broth was superior to TSC agar for enumerating and confirming the small numbers of C. perfringens that were present in a majority of the samples. This was especially true when other clostridia were also present. Besides its greater selectivity and sensitivity, LS broth had the additional advantages of requiring less work and giving confirmed results within 24-48 h compared with 3 days for the TSC agar method.

Agar↗

PubMedDate not supplied

[Identification of Ristella biaticus (Prévot 1967) (ex Fusiformis biaticus, Prévot) with Clostridium microsporum (Spray 1947)].

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Bacteroides↗

PubMedDate not supplied

[Humanization of milk: "in vitro" influence of human milk, cow milk and infant formulae on the growth of "Bifidobacterium" (author's transl)].

Fresh human milk not only influence the physicochemical conditions in the infant's intestine, but also enhances the in vitro growth of Bifidobacterium Various Bifidobacterium strains belonging to different species can be clearly distinguished by their growth properties with different kinds of milk. Only B. bifidium, the predominant species in the intestine of breast-fed infants, is stimulated by human milk.

Actinomycetaceae↗