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H Bauer

Publications and source records attributed to H Bauer.

549 records · Page 31Linked to original sources
PubMedDate not supplied

Experimental Gd-DTPA polylysine enhanced MR angiography: sequence optimization.

Most MR angiography (MRA) techniques use macroscopic blood motion to characterize the flowing spins. A different approach is represented by the use of contrast enhancement of blood pooling agents. The intravenous injection of one of these agents, namely Gd-DTPA polylysine, produces a shortening of the blood T1 below the T1 values of fat and soft tissues. In this study on experimental MRA in rabbits, we used an imaging sequence with a 90 degrees saturation pulse and a 180 degrees inversion recovery pulse. Both the saturation and inversion recovery times were adjusted to suppress the signals of soft tissues and fat. The remaining ultrashort T1 blood was imaged with a projective velocity refocused spin echo or gradient echo sequence. Magnetic resonance angiography provided excellent vessel detail with a 30-60 s acquisition time. In addition, there was no need for further processing after image acquisition because the projection was obtained immediately. The disadvantages of the technique are the need for contrast medium injection and the nonselectivity of MRA.

Animals↗
PubMedDate not supplied

Incorporation of beta-galactosidase-expressing endothelial cells into the skeletal muscle microvascular bed of mice.

Cloned murine endothelial cells (cEC) were used as a carrier system for introducing a foreign gene into the microvascular bed of the hind limb of inbred mice. cEC were transfected with a beta-galactosidase-neo fusion construct, which enables both selection for DNA uptake in the presence of G 418 and the staining of cells for beta-galactosidase activity. Transfected cEC adhered and integrated readily into confluent monolayers of nontransfected cEC (up to 26% of total cell number). Seeding lacZ-transfected cEC on explanted arteries revealed rapid adhesion of the cells (within minutes) to the intact endothelium. After injection of 10(6) transfected EC via the femoral artery into the microvascular bed of the hind limb their presence was documented by beta-galactosidase staining after various time periods (1 h to 4 wk). Implanted cEC were detected in numerous elements of the microcirculation both in frozen sections and in squash preparations of the hind limb muscle and in the femoral bone up to 4 wk after the injection. The microvascular bed of skeletal muscle of the mouse as a recipient site for transduced syngeneic endothelial cells is, thus, a suitable experimental model to study various strategies for somatic gene therapy.

Animals↗
PubMedDate not supplied

Evaluation of proteinuria by two-dimensional polyacrylamide gel electrophoresis after kidney transplantation.

Urinary proteins were studied in patients after kidney transplantation during various functional states (normal function, acute rejection, chronic rejection) by two-dimensional polyacrylamide gel electrophoresis. In the first dimension, the proteins were separated according to their electric charge by isoelectric focusing, and in the second dimension according to their molecular weight by sodium dodecyl sulfate polyacrylamide gel electrophoresis. After electrophoresis the proteins were visualized using a highly sensitive silver stain technique. The combination of these methods allowed the discrimination of up to 250 protein spots in human urine, most of them unidentified up to now. Functional changes of the kidney transplants were accompanied by complex changes of the protein pattern in urine. These changes cannot be simply compared to the tubular and glomerular pattern of proteinuria which can be identified by one-dimensional sodium dodecyl sulfate electrophoresis. The 2D electrophoresis of urinary proteins may develop into a useful tool in localizing of kidney damage and in the evaluation of renal disease.

Albuminuria↗
PubMedDate not supplied

Epidemiologic study of prostatic cancer: preliminary report.

In a preliminary report on a small fraction of the projected subject population, some evidence in support of both sexual activity and venereal transmission hypotheses is accumulating. Prostatic cancer cases, in contrast to hospitalized and/or neighborhood controls, are beginning to show greater proportions of selected sexual activities compatible with venereal transmission of an infectious agent, such as number of sexual partners, use of prostitutes, prior venereal disease, and genital infections in the spouse. Patients with prostatic cancer also appear to have had higher fertility and more prostatic cancer in blood relatives than controls. Age at first intercourse and at first marriage are lower among the cancer patients than among the controls. Antibody titrations for herpesvirus and cytomegalic virus, although currently not revealing striking disparities in positivity, tend to show higher titers among the cancer cases.

Adult↗
PubMedDate not supplied

[Premorbid psychological processes in patients with Alzheimer's disease and in patients with vascular dementia].

We analyzed the premorbid biographies of 21 patients with Alzheimer's disease for possible common features. Eleven age-matched patients suffering from vascular dementia served as a control group. The observations from our qualitative study indicate that persons with a conflict-avoiding, submissive, premorbid personality predominate among Alzheimer patients. Persons who later became Alzheimer patients tended to leave important daily-life decisions to their partners (or other persons of reference). Prior to the onset of the very first neuropsychological deficits, persons who later became Alzheimer patients were found to stay in a lasting situation in which they were subject to a treatment that could be designated as "caring tutelage." Subsequently, most patients became subject to an increasingly patronizing and restricting treatment. Further elements that were frequently found as part of the premorbid development were physical or psychological burden, loss of social contacts, and loss of motivation. In contrast, assertive and dominant premorbid traits predominated in the group of vascular patients. The premorbid biographical situation of persons who later became vascular patients was characterized by a loss of the control which these persons had hitherto exerted over partners, their families, or the situation at their working place. We advance the hypothesis that in Alzheimer's disease the described psychological phenomena are part of a preclinical process, during which biological, psychological, and social factors interact, finally joining into the clinical stage of the disease. Possibilities for psychotherapeutic interventions are discussed.

Adult↗