Isolation and analysis of trienoic and tetraenoic fatty acids with complementary thin layer and gas-liquid chromatography.
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Biomedical subjects
Publications and source records attributed to H B White.
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A method is described for the simple and rapid formation of saturated fatty aldehydes from the corresponding acid chlorides. It is not suitable for the preparation of unsaturated aldehydes because of the partial reduction and positional and geometrical isomerization of the double bond in the chain.
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Fatty acid methyl esters were separated into fractions according to chain length on a nonpolar gas-liquid chromatographic column. These fractions were collected and rechromatographed on a polar column. Temperature programming was used in both cases. Data are given for the accuracy of the double procedure applied to a synthetic mixture.
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