Effect of ionic strength, pH, amines and divalent cations on the lytic activity of T4 lysozyme.
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Amines↗
Biomedical subjects
H B Jensen
Publications and source records attributed to H B Jensen.
Studies on T4 lysozyme. Affinity for chitin and the use of chitin in the purification of the enzyme.
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Acrylamides↗
The appearance of phage associated lysozyme in E. coli B cells immediately after infection with phage T2.
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Cell-Free System↗
Vitamin A and carotenoids in the liver of mammals, birds, reptiles and man, with particular regard to the intensity of the ultraviolet absorption and the Carr-Price reaction of vitamin A.
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Journal Article↗
Molecular analysis of an outer membrane protein, MopB, of Methylococcus capsulatus (Bath) and structural comparisons with proteins of the OmpA family.
The gene encoding a major outer membrane protein (MopB) of the methanotroph Methylococcus capsulatus (Bath) was cloned and sequenced. The cloned DNA contained an open reading frame of 1044 bp coding for a 348-amino-acid polypeptide with a 21-amino-acid leader peptide. Comparative sequence analysis of the predicted amino acid sequence revealed that the C-terminal part of MopB possessed sequences that are conserved in the OmpA family of proteins. The N-terminal half of the protein had no significant sequence similarity to other proteins in the databases, but the predicted secondary structure showed stretches of amphipathic beta-strands typical of transmembrane segments of outer membrane proteins. A region with four cysteines similar to the cysteine-encompassing region of the OprF of Pseudomonas aeruginosa was found toward the C-terminal part of MopB. Results from whole-cell labeling with the fluorescent thiol-reacting reagent 5-iodoacetamidofluorescein indicated a surface-exposed location for these cysteines. A probe consisting of the 3'-end of the mopB gene hybridized to the type I methanotroph Methylomonas methanica S in Southern blots containing DNA from nine methanotrophic strains representing six different genera.
Alphaproteobacteria↗