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Biomedical subjects

H Arvilommi

Publications and source records attributed to H Arvilommi.

88 records · Page 5Linked to original sources
PubMed1977

A simple technique for measuring leucocyte chemotaxis in reversible Boyden chambers.

A technique for assay of leucocyte chemotaxis in Boyden chambers is described. A set of up to 12 closed chambers with 2 compartments is formed from commercially available migration plates. The set can be turned during the incubation and thus the detachment of cells from the filter, a major source of variation in classical type of chambers, can be prevented. The technique is simple and so inexpensive that it allows the use of the material as disposable if preferred.

Chemotaxis, Leukocyte↗
PubMed1976

Grouping of beta-haemolytic streptococci by using coagglutination, precipitation or bacitracin sensitivity.

This study was made to compare coagglutination to precipitin test in grouping beta-haemolytic streptococci from clinical specimens and to investigate the accuracy of the bacitracin test in identification of group A streptococci. Results of grouping 126 strains with coagglutination and precipitation were identical in all except two cases. These two strains were nongroupable with precipitation but appeared as group B and C by coagglutination. When the distribution of group A, B, C and G streptococci in various clinical sources was investigated it was found that group B strains were the most frequently (41 per cent) isolated streptococci and even in isolates from pharyngeal swabs their proportion was 33 per cent. The accuracy of the bacitracin test in identification of group A streptococci was unsatisfactory as 26/62 (42 per cent) strains reported as group A by using this test were in fact group B, C or G streptococci. One of the reasons for this high number of false positives appeared to be the medium used for the preparation of the blood agar plates. In view of the frequent occurrence of non-A-streptococci in clinical specimens and high incidence of false positive in the bacitracin test it is suggested that this test should be replaced by a more efficient method of serological grouping.

Agglutination Tests↗
PubMed1976

Sulphamethoxazole-trimethoprim: effect on antibody response in man.

The possible immunosuppressive properties of the combination sulphamethoxazole-trimethoprim or either of the agents alone were studied in healthy human volunteers. The effect of the 4-day drug treatment on the antibody synthesis after vaccination was measured by titrating tetanus antitoxin, mumps antibody and Salmonella Hb antibody levels 4 and 5 weeks after vaccination. No significant differences were found in mumps or Salmonella antibody titres in the drug-treated group compared to controls. Tetanus antitoxin levels, however, were significantly lower than in controls after the combined drug. When a fourfold or greater increase in tetanus antitoxin titre was obtained in 75% of controls, the respective figures were 38% in the sulphamethoxazole-trimethoprim-treated group (p less than 0.05), 45% in the trimethoprim group (p greater than 0.05), and 50% in the sulphamethoxazole group (p greater than 0.05). The implications of these findings are discussed.

Antibodies, Bacterial↗
PubMed1975

A method for simultaneous determination of rosette formation and phagocytosis by cells.

A fluorescent agent insoluble in water was readily phagocytosed by 5-20% of human blood mononuclear cells, mainly monocytes. These cells were easily detected even amidst abundant rosetting erythrocytes. When rosette assays for the detection of complement or SRBC receptors on the surface of the cells were performed, only phagocytic cells exhibited the complement receptor with the method used in the study, whereas mainly nonphagocytic cells formed SRBC rosettes. The effect on the calculation of the percentage of T lymphocytes (SRBC receptor lymphocytes) of varying amounts of monocytes present in mononuclear cell population is demonstrated. The method presented here might prove useful particularly in studies on Fc or complement receptors on white blood cells.

Animals↗
PubMedDate not supplied

Rapid identification of Bordetella pertussis pertactin gene variants using LightCycler real-time polymerase chain reaction combined with melting curve analysis and gel electrophoresis.

Recently, eight allelic variants of the pertactin gene (prn1-8) have been characterized in Bordetella pertussis strains isolated in Europe and the United States. It has been suggested that the divergence of the pertactin types of clinical isolates from those of the B. pertussis vaccine strains is a result of vaccine-driven evolution. Sequencing of the prn, which is relatively time-consuming, has so far been the only method for the differentiation of prn types. We have developed a rapid real-time polymerase chain reaction assay suitable for large-scale screening of the prn type of the circulating strains. This method correctly identified the prn type of all tested 41 clinical isolates and two Finnish vaccine strains. The method is simple and reliable and provides an alternative for sequencing in pertussis research.

Amino Acid Sequence↗