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Biomedical subjects

H Adachi

Publications and source records attributed to H Adachi.

At least 577 records · Page 32Linked to original sources
PubMed1978

Sandwich enzymoimmunoassay of hepatitis B surface antigen (HBsAg).

A sandwich enzymoimmunoassay (EIA) procedure was developed for the detection of HBsAg using Fab' of anti-HBsAg conjugated with beta-D-galactosidase from Escherichia coli with anti-HBsAg-coated silicone rubber discs as a solid phase. EIA could detect 1 ng/ml of HBsAg. It was as sensitive as radioimmunoassay (RIA, AusRia II) and about 30-fold more sensitive than reversed passive hemagglutination assay RPHA, ReverseCell). EIA and RIA could detect more HBsAg-positive sera than RPHA.

Galactosidases↗
PubMed1977

An ultrastructural study of the red pulp of the spleen and the liver in unstable hemoglobin hemolytic anemia.

Electron microscopic observations on the process of red cell destruction in the spleen and liver of a patient with congenital Heinz body hemolytic anemia, associated with a new variant of unstable hemoglobin, are reported. Two major mechanisms of destruction of Heinz body-containing red cells were noted. One was phagocytosis of these cells in toto by cordal macrophages. The other mechanism though less significant quantitatively, was intravascular hemolysis of injured red cells in the splenic microvasculature. In the liver, phagocytosis of damaged red cells by Kupffer cells was rate and there was no evidence of intravascular hemolysis in this organ. These morphological findings, together with almost complete recovery from hemolysis following splenectomy, indicated that Heinz body-containing red cells were removed from the circulation predominantly by the spleen. In contrast to experimental Heinz body anemia in animals, Heinz bodies were present even in the nucleated cells.

Anemia, Hemolytic↗
PubMed1977

Pathological study on experimental Heinz body anemia: intracellular changes of phagocytized red cells in macrophages of the spleen and liver.

Heinz body anemia was experimentally produced in mice by phenylhydrazine injection and the mechanism of hemolysis was investigated. Both in acute and chronic experiments, the "culling" function of the spleen was more important in removing the red cells containing Heinz bodies than the "pitting" function. In addition to extravascular hemolysis, intravascular hemolysis in the spleen, liver and in the peripheral circulation was evident as manifested by the presence of ghost cells and hemosiderin deposition in the kidney. In the absence of the spleen, the liver can replace the function of the spleen to a certain extent and selective removal of Heinz bodies by the Kupffer cell was occasionally demonstrated. Deposition of yellowish-brown pigments was noted in the cordal macrophages and the Kupffer cells, but the precise nature of these pigments is unknown.

Acute Disease↗
PubMed1977

Axial transverse tomography of the cervical spine narrowed by ossification of the posterior longitudinal ligament.

Ossification of the posterior longitudinal ligament in the cervical region is generally regarded as a rare disease, though a Japanese series of 185 cases has been reported. The main symptoms arise from a myelopathy, the degree of which varies from moderate to severe, due to stenosis of the spinal canal. In order to clarify the relationship between the severity of symptoms and the cross-sectional area of ossification, axial transverse tomography of the cervical spine has been carried out on twenty-six patients at intervals of 5 millimetres over the full extent of the ossification. In this way the cross-sectional areas of the ossified tissue and of the spinal canal were calculated. The former varied from a minimum of 0.8 to a maximum of 1.8 square centimetres. In cases of severe myelopathy the ossification was mainly at the fourth and fifth cervical levels and the stenosis ration exceeded 30 per cent.

Adult↗