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Biomedical subjects

H Adachi

Publications and source records attributed to H Adachi.

At least 289 records · Page 16Linked to original sources
PubMed1994

Identification of estrogen receptor in human adipose tissue and adipocytes.

Estrogen has various effects on adipose tissue. Although the presence of estrogen receptor (ER) has been demonstrated in rat adipose tissue and adipocytes, ER has not been identified in human adipose tissue. In this study, we demonstrated the existence of ER protein and ER messenger RNA (mRNA) in human sc adipose tissue and adipocytes. The cytosol fraction of human adipose tissue was partially purified by ammonium sulfate precipitation, and the presence of ER protein was analyzed by [3H]estradiol (E2) binding assay and Western blot analysis. [3H]E2 binding assay showed a low specific binding due to high nonspecific binding, and the dissociation constant (Kd) and maximal binding sites could not be obtained by Scatchard analysis. Western blots, however, showed the presence of ER protein in both the partially purified cytosol and nuclear fractions of human adipose tissue. The mol wt of ER in both fractions was approximately 66,000. Furthermore, Northern blot analysis of total RNA samples isolated from human adipose tissue showed the expression of ER mRNA at 6.2 kilobase in size. ER mRNA was also identified in isolated human adipocytes by the reverse transcription and polymerase chain reaction. These results indicated that both ER protein and ER mRNA are expressed in human adipocytes, suggesting that the effect of estrogen on human adipose tissues might involve a direct action.

Adipocytes↗
PubMed1994

Involvement of epidermal growth factor (EGF)/EGF receptor autocrine and paracrine mechanism in human trophoblast cells: functional differentiation in vitro.

We have studied the expression of epidermal growth factor (EGF) and EGF receptors (EGF-R) in isolated human trophoblast cells at various stages of differentiation and also the biological significance of the EGF/EGF-R autocrine and paracrine mechanism. Cytotrophoblast cells were isolated from human placental tissues of 6-9 weeks of gestation. Trophoblast cells underwent morphological and functional differentiation during in vitro culture. The expression of EGF and EGF-R protein and mRNA was studied in trophoblast cells cultured for 0-5 days, using immunocytochemical staining, and reverse transcription and polymerase chain reaction. Monoclonal antibodies (mAbs) against EGF and EGF-R showed specific staining in trophoblast cells at all stages of differentiation. Both EGF and EGF-R gene transcripts were detected in RNA samples isolated from trophoblast cells at all stages. These data suggest the presence of an EGF/EGF-R autocrine and paracrine mechanism in human trophoblast cells. Next, we examined the biological significance of this mechanism on trophoblast cell differentiation in vitro. EGF added to the culture medium significantly increased human chorionic gonadotrophin-beta (hCG-beta) secretion and, more importantly, anti-EGF neutralizing mAbs significantly reduced both hCG-beta and human placental lactogen secretion from trophoblast cells in culture. All these results suggest that human trophoblast cells express both EGF and EGF-R, and that EGF may play an important role in the functional differentiation of human trophoblast cells.

Base Sequence↗
PubMed1994

Effects of 3'-angeloyloxy-4'-acetoxy-3',4'-dihydroseselin on cardiohemodynamics in anesthetized dogs.

Cardiohemodynamic effects of 3'-angeloyloxy-4'-acetoxy-3',4'-dihydroseselin (Pd-Ia) isolated from the Chinese medicinal plant Peucedanum praeruptorum Dunn were compared with those of diltiazem (Dil) in anesthetized open-chest dogs. Pd-Ia 3 mg.kg-1 increased coronary blood flow from 25 +/- 11 to 58 +/- 16 ml.min-1 (n = 7, P < 0.01) and decreased mean aortic pressure (MAP) from 13 +/- 2 to 8 +/- 1 kPa (P < 0.01), rate pressure product (RPP) from 1.9 +/- 0.5 to 1.3 +/- 0.3 MPa.bpm (P < 0.05), +dp/dtmax from 246 +/- 56 to 160 +/- 36 kPa.s-1 (P < 0.01) and systemic vascular resistance from 19 +/- 4 to 12 +/- 7 Pa.ml-1.min-1 (P < 0.05), together with an increase in HR. Dil showed effects similar to those of Pd-Ia except for a marked decrease in HR. The effects of Pd-Ia on MAP and RPP were approximately one-tenth as potent as those of Dil. The results demonstrated that Pd-Ia was a Ca2+ channel blocker.

Animals↗
PubMed1994

Effects of 3'-angeloyloxy-4'-acetoxy-3',4'-dihydroseselin on myocardial dysfunction after a brief ischemia in anesthetized dogs.

The effect of a 30-min infusion of 3'-angeloyloxy-4'-acetoxy-3', 4'-dihydroseselin (Pd-Ia), a coumarin isolated from Peucedanum praeruptorum Dunn 0.15 mg.kg-1.min-1 on regional myocardial dysfunction was examined in 16 anesthetized open-chest dogs subjected to a 15-min occlusion of the left anterior descending coronary artery followed by a 3-h reperfusion. Segment lengths of left ventricular wall were measured with an ultrasonic micrometer. The control caused a decrease in the % of segment shortening (SS%) throughout the reperfusion period (n = 8), while Pd-Ia ameliorated segment function immediately after reperfusion and restored the SS% to 41 +/- 51% of the baseline value (n = 8, P < 0.05 vs control) 5 min after reperfusion without any significant changes in cardiohemodynamics. The improvement of myocardial function induced by Pd-Ia was maintained at least 3 h after reperfusion. These findings revealed that Pd-Ia had a cardioprotective action in stunned myocardium.

Animals↗
PubMed1994

[A case report of tricuspid valve valvoplasty for tricuspid endocarditis].

A successful repair of tricuspid valve insufficiency due to endocarditis related to VSD is reported. Combined quadrangular resection and repair of anterior leaflet, pericardial patch plasty of anterior-septal commissure, Carpentier ring annuloplasty and VSD closure was performed with good clinical results.

Adult↗
PubMed1994

[Synergistic effect of carboplatin and hyperthermia in rat and human glioma cell lines].

To assess the interaction of carboplatin and hyperthermia in vitro, the thermochemosensitivities of three glioma cell lines, C6 rat glioma cell line, human glioma cell lines T98G and KMG4, were examined by 3-(4,5-dimethylthiazol-2-yl) -2,5 diphenyltetrazolium bromide (MTT) assay. The cell survival of each cell line decreased according to increasing CBDCA concentration and temperature. With a certain CBDCA concentration, the cell survival at following temperature was significantly decreased from that at 37 degrees: 43 degrees C and 44 degrees C for C6 cells (2.5 micrograms/ml); 41 degrees C, 42 degrees C, 43 degrees C and 44 degrees C for C6 cells (128 micrograms/ml); 42 degrees C, 43 degrees C and 44 degrees C for KMG4 cells (8 micrograms/ml) (CBDCA concentration within parentheses). It is generally considered that the highest tolerable temperature of normal brain is 42 degrees C for 60 minutes, while under 43 degrees C, there is a possibility that a sufficient tumoricidal effect might not be obtained. This study revealed enhanced cytotoxicity of CBDCA with hyperthermia at the temperature lower than 42 degrees C and suggests the possibility to gain increased tumoricidal effect without injuring normal brain by hyperthermia at the normal-tissue-tolerant temperature with systemic administration of relatively lower dose of CBDCA.

Animals↗
PubMed1994

Telepathology is available for transplantation-pathology: experience in Japan using an integrated, low-cost, and high-quality system.

We examined the validity and accuracy of telepathology service in the histological diagnosis of biopsy specimens from human transplanted kidney and liver. The still video images of paraffin sections were transmitted via a two-way telephone by use of a digitized telephone network (Integrated Service Digital Networks, 64 kbits/sec). The images were displayed on monitors and diagnosed by an expert pathologist at Tottori University. The quality of transmitted still images was sufficient for the diagnosis, especially at higher magnifications. The average number of transmitted images was 6.2 in the kidney cases and 7.4 in the liver cases. The average time taken for examination of a case was 13 min (range 10 to 16 min). Of 12 biopsy specimens from transplanted kidneys, 10 were adequately diagnosed with the system. Sampling errors caused inadequate diagnosis in a case of cyclosporin tubulopathy, the still images of which were not transmitted. An expert pathologist rendered the diagnosis in a case showing mesangial sclerosis, which was later diagnosed as possible recurrent glomerulonephritis through direct microscopy. Biopsy specimens from 10 liver transplants were also tested using archival materials. Although the etiology of hepatitis could not be determined in one case, diagnoses by telepathology well agreed with the reported diagnoses made through direct microscopy. Telepathology may be an effective way to provide on-line consultations in transplantation pathology, especially for transplant teams lacking expert pathologists.

Adolescent↗
PubMed1994

[Reoperation for recurrent chondrosarcoma of the pulmonary trunk].

A 71-year-old woman who had progressive dyspnea and edema two years after resection of a pulmonary trunk chondrosarcoma was diagnosed as severe obstruction to the pulmonary trunk due to the recurrent tumor. Magnetic resonance examination following the intravenous administration of Gd-diethylenetriamine pentaacetic acid (DTPA) revealed striking contrast enhancement within the lesion of the pulmonary trunk sarcoma. At reoperation, the recurrent mass was identified within the same site of the pulmonary trunk and total resection of the tumor and replacement of the pulmonary trunk with prosthetic graft was performed utilizing a cardiopulmonary bypass. There have been very few reports on replacements of pulmonary trunks for pulmonary artery sarcomas. It is reported that the prognosis of the pulmonary trunk sarcoma is extremely poor, however, this patient has undergone thoracotomies twice within two years and continued to be symptom-free without distant metastatic lesions.

Aged↗
PubMed1994

[Preoperative assessment of aortic dissection by three-dimensional CT angiography].

In 21 patients with aortic dissection including 12 patients with acute aortic dissection, aortic lesions were evaluated preoperatively by newly developed three-dimensional CT angiography (3 D-CTA) using helical scan CT. Rapid and safe three-dimensional evaluation of aortic dissection was achieved with no complications. Three-dimensional structure of aortic dissection was well reflected in the image of 3D-CTA. Preoperative three-dimensional evaluation was useful to determine and proceed the operative procedure for the repair of aortic dissection. All patients were alive and results of surgical treatments were excellent. Intimal flaps and entries were visualized by multiple threshold display (MTD) method of 3D-CTA. Multiple threshold display method is an important three-dimensional reconstructive method and 3D-CTA may be a new useful and powerful diagnostic modality for aortic dissection.

Adult↗
PubMed1994

[Evaluation of the addition of counterpulsation to the partial left ventricular-femoral bypass for limitation of evolving myocardial infarction].

The effect of adding counterpulsation to a partial left ventricular bypass was evaluated in a canine model of acute myocardial ischemia by using a myocardial staining method. To establish a left ventricular bypass, a catheter consisting of bypass tube (90 cm in length and 15 Fr in inner diameter) and an accompanying intraaortic balloon as a single apparatus (Integrated Cardioassist Catheter; ICAC) was introduced into the left ventricle via the abdominal aorta. The left ventricular bypass was adjusted to 1 L/min. with or without counterpulsation with the aid of a centrifugal pump and IABP console. The use of the ICAC that provided pulsatile left ventricular bypass tended to raise the mean aortic pressure and cardiac output. Reduction of the tension time index was noted with the use of the ICAC in contrast to the control. And increment of the DPTI/TTI ratio was observed with the use of the ICAC in contrast to the control and partial left ventricular bypass alone. While the percentages of the region at risk were similar [17.3 +/- 9.5% (control), vs. 16.4 +/- 3.4% (partial left ventricular bypass alone) vs. 16.9 +/- 5.2% (ICAC)], the percentages of infarct sizes were reduced due to the use of counterpulsation. [61.9 +/- 12.2% (control), vs. 57.5 +/- 3.9% (partial left ventricular bypass alone) vs. 16.8 +/- 6.0% (ICAC)]. These results revealed that the addition of counterpulsation alleviates afterload reduction to the partial left ventricular bypass and was more beneficial to the reduction of the infarct size than partial left ventricular bypass alone.

Animals↗
PubMed1994

[Aortic valve replacement for a patient associated with severe liver cirrhosis].

A 55-year-old man who had severe aortic regurgitation by bacterial endocarditis associated with severe alcoholic liver cirrhosis admitted in our hospital for the treatment of congestive heart failure. ICG15' score was 45.0% and redox tolerance test score was 0.38 x 10(-2). Preoperative hepatic function state was estimated as Child B. Preoperative preparation for the liver cirrhosis was done with FFP and glucagon insulin treatment. Aortic valve replacement was performed under the pulsatile high flow and high perfusion pressure during cardiopulmonary bypass. Post operative course was fair and he discharged.

Aortic Valve↗
PubMed1994

[Operative strategy for redo heart valve operation].

Thirty-five redo heart valve operations were performed between Jan. 1990 and Dec. 1993 in Jichi Ohmiya Medical Center. Previous operations were mitral valve replacement (MVR) in 19, closed mitral commissurotomy in 1, open mitral commissurotomy in 6, aortic valve replacement (AVR) in 5, ASD repair in 3, VSD repair in 1. Thirty (85.7%) were in NYHA functional class III or less, and 5 (14.3%) were in functional class in IV. Twenty-three patients had undergone MVR, 5 AVR, 4 MVR+AVR, 2 TVR (TAP), 1 Cabrol operation, and 8 patients had undergone concomitant tricuspid valve repair. Emergent operation was none. Overall operative mortality was 5.7% (2/35), as the cause of death was low output syndrome. Chest CT was available for the evaluation of the distension of right atrium and right ventricle. Catastrophic hemorrhage by the injury of ascending aorta or right atrium during redo sternotomy was 4, but that was not the cause of death. We emphasize the necessity of the exposure of the femoral artery and the preparation for cardiopulmonary bypass before sternotomy. The extent of dissecting adhesion should be determined case by case respectively under consideration of the risk of cardiovascular injury and postoperative large bleeding. Furthermore the cannule of SVC or IVC with cuff was useful for the cases with strong adhesion.

Adult↗
PubMed1994

[Clinical pharmacokinetics of carboplatin and MCNU in malignant brain tumor and normal brain tissues].

We measured the concentrations of MCNU and CBDCA in the serum, brain tumor and normal brain tissue. Six patients with malignant glioma were treated with intravenous chemotherapy using 80mg/m2 MCNU and 300mg/m2 CBDCA during surgery. After drug administration, specimens of serum, tumor and normal brain tissue were collected every 30 min and then the drug concentration in each sample was measured. The highest MCNU levels in all samples were obtained immediately after administration which followed by gradual decrease. On the contrary, the mean CBDCA levels in the tumor and normal tissue remained almost at a constant level, although serum CBDCA level declined rapidly as MCNU. As expected, MCNU seemed to have advantages in the treatment of brain tumors as it distributed with higher concentration in the tumor tissue than in the serum and normal brain tissue. On the contrary, CBDCA in the tumor tissue did not exceed the concentration in the serum. Nevertheless, it remained longer in the tumor tissue with a constant level, suggesting that CBDCA can achieve an effective area under the concentration versus time curve (AUC) in the brain tumor tissue to kill tumor cells.

Aged↗
PubMed1994

[A case of pineal region germinoma treated by interstitial brachytherapy].

Although radiation therapy has been universally accepted as treatment for primary intracranial germinomas, the optimal technique for both diagnosis and treatment continues to be a matter of controversy. We report a case of germinoma in the pineal region treated by interstitial brachytherapy. A 23-year-old female was admitted with diplopia and consciousness disturbance. Magnetic resonance imagings (MRI) revealed an enhanced tumor of the pineal region and hydrocephalus. No serum and CSF tumor markers such as alpha-fetoprotein (AFP), human chorionic gonadotropin (HCG) or placental alkaline phosphatase (PLAP) were detectable. Stereotactic needle biopsy was performed using BRW MRI-guided stereotactic apparatus. After the histological diagnosis confirmed the presence of germinoma, three catheters for interstitial brachytherapy were implanted into the tumor through the biopsy tract, and iridium-192 seeds were inserted into the catheters and kept there for 5 days to give 35 Gy of irradiation at the tumor periphery. Subsequent MRI showed marked tumor regression and the clinical symptoms were gradually improved, and Gd-enhanced tumor on MRI completely disappeared 2 months after brachytherapy. Histological diagnosis is essential before initiation of treatment because a germinoma is commonly thought to be radiosensitive. However, pineal region tumor is dangerous to remove surgically because of high morbidity and mortality rates. Image-guided stereotactic technique provides a safe method to obtain an accurate diagnosis, and subsequent interstitial brachytherapy enables selective irradiation of the tumor, and does not give rise to complications caused by external irradiation such as mental disturbance, hypopituitarism, visual disturbance and brain necrosis. Interstitial brachytherapy following image-guided biopsy can be a useful tool for patients with germinoma.

Adult↗
PubMed1994

[A case of primary angiosarcoma of the heart].

We report a case of angiosarcoma of the right atrium, manifested as a cardiac tamponade. The patient was a 57-year-old man. Transesophageal echocardiographic examination revealed a pericardial effusion and an abnormal structure arising in the right atrium. Emergency operation was performed and the tumor in the right atrium was resected under cardiopulmonary bypass. The resected tumor measured 5.0 x 3.5 x 2.0 cm, and microscopic examination revealed an angiosarcoma. The patient was initially recovered, but he died of heart failure caused by the recurrence in the left ventricle on the 5th postoperative month.

Cardiac Tamponade↗
PubMed1994

[Evaluation of myocardial perfusion and ventricular shape in hypertrophic cardiomyopathy using 99mTc-tetrofosmin scintigraphy: comparison with 201Tl myocardial scintigraphy].

Hypertrophic cardiomyopathy (HCM) is known to have the impairment of myocardial perfusion as well as irregularly hypertrophic myocardium. To evaluate myocardial perfusion and ventricular shape in HCM, 99mTc-Tetrofosmin scintigraphy was performed after exercise (Ex) and at resting state (Re) in 10 patients with HCM and was compared with early image (Ea) and delayed image (De) of 201Tl scintigraphy performed after exercise. SPECT images of both 99mTc-Tetrofosmin and 201Tl scintigraphy were analyzed with five scaled visual scores set in 18 segments. The complete concordance ratio between 99mTc-Tetrofosmin (Ex and Re) and 201Tl (Ea and De) images in segmental analysis was 75%. Image quality of 99mTc-Tetrofosmin was seemed to be superior to that of 201Tl scintigraphy. In 9 patients with HCM, 99mTc-Tetrofosmin scintigraphy was performed under the ECG gating and the thickness of septal and free wall was measured. Good correlation was observed with the data by ultrasound cardiography (r = 0.79, p < 0.002 in wall thickness, r = 0.84, p < 0.01 in the ratio of septal wall thickness to free wall thickness). Left ventricular shape (ventricular long axis) was closely resemble to that of left ventriculography by contrast medium. In conclusion, 99mTc-Tetrofosmin scintigraphy is useful for the evaluation of myocardial morphology as well as perfusion abnormality.

Adult↗
PubMed1994

[Replacement of ascending and proximal transverse aorta for a 90-year-old female with aortic dissection].

Successful replacement of ascending and proximal transverse aorta for a 90-year-old female with aortic dissection was achieved using selective cerebral perfusion with cold blood. The ascending aorta enlarged as a diameter of 7 cm and massive blood flow was recognized in the false lumen detected by CT scanning and echocardiography. After the core cooling by cardiopulmonary bypass (CPB), the branches of the aortic arch were cannulated and perfused with cold blood (16 degrees C) for cerebral protection. The time of CPB, cardiac arrest and selective cerebral perfusion was 153 min, 92 min and 35 min, respectively. Post operative course was uneventful. She discharged from the hospital 4 weeks after the operation.

Aged↗